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    Wyświetlanie 1-6 z 6
    Tytuł:
    Polymorphism in Syringa rDNA regions assessed by PCR technique
    Autorzy:
    Smolik, M.
    Andrys, D.
    Franas, A.
    Krupa-Malkiewicz, M.
    Malinowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41641.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    polymorphism
    lilac
    Syringa
    rDNA region
    polymerase chain reaction
    Opis:
    The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.
    Źródło:
    Dendrobiology; 2010, 64
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of microsatellite markers for horse-chestnut (Aesculus hippocastanum), their polymorphism in natural Greek populations, and cross-amplification in related species
    Autorzy:
    Walas, L.
    Iszkulo, G.
    Barina, Z.
    Dering, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2078223.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Tertiary relict
    endemic species
    cross-amplification
    polymorphism
    Opis:
    New nuclear microsatellite markers (SSRs) were developed for Aesculus hippocastanum, a relict tree species from the Balkan Peninsula. The development of microsatellites was done using the Illumina MiSeq PE300 platform. Out of a set of 500 SSRs designed, a subset of 13 loci was tested using 290 individuals from seven natural populations. Twelve species-specific loci were polymorphic. The number of alleles per locus ranged from 2 to 17 and expected heterozygosity from 0.089 to 0.800 with a mean value of 0.484. The population of Kalampaka had the lowest value of allelic richness (2.63) and gene diversity in comparison to the remaining populations. STRUCTURE analysis confirmed isolation of population Mariolata from the southern edge of the species range and genetic similarity among populations from the Pindos Mts. Additionally, the utility of new SSRs in 29 individuals from nine other Aesculus taxa was tested. Eleven markers gave polymorphic products for all tested species. For 24 individuals, a high-quality product was obtained for each marker. Results confirmed the utility of specific markers for future population genetics studies.
    Źródło:
    Dendrobiology; 2021, 85; 105-116
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of SNPs based on DNA specific-locus amplified fragment sequencing in Chinese fir (Cunninghamia lanceolata (Lamb.) Hook)
    Autorzy:
    Su, Y.
    Hu, D.
    Zheng, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/41243.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    detection
    single nucleotide polymorphism
    DNA specific-locus amplified fragment sequencing
    fir
    Chinese fir
    Cunninghamia lanceolata
    genotyping
    Opis:
    Compared to angiosperms, conifers represent more complex genomes with larger giga-genome size. To detect large-scale single nucleotide polymorphisms (SNPs), whole genome sequencing of a conifer population is still unaffordable. In this work, we report the use of DNA specific-locus amplified fragment sequencing (SLAF-seq) for large-scale SNP detection in Chinese fir (Cunninghamia lanceolata (Lamb.) Hook), an ecological and economic important conifer in China. SLAF libraries of 18 parent clones of a Chinese fir 2.5 generation seed orchard were sequenced and a total of 117,924 SLAFs were developed. We detected 147,376 SNPs from these SLAFs; 146,231 of them represented simple nucleotide change in A/G, C/T, A/C, A/T, C/G or G/T. The most frequent SNPs occurred in C/T (34.3%), while the majority of SNPs (68.2%) belonged to transition events (A/G and C/T). Notably, all the sequenced samples had high portion (78.2–80.9%) of common SNPs indicating that the Chinese fir genomes tended to change its nucleotides at common loci. 48,406 informative SNPs were then successfully utilized to genotype the tested samples (n = 18) followed by a phylogenetic tree to clarify their genetic relationship. Furthermore, a set of very high linkage disequilibrium (0.51–1.00) were identified from these informative SNPs. In brief, our work demonstrated that SLAF-seq is an alternative and cost-effectively high-throughput approach for large-scale SNP exploitation in Chinese fir. While the obtained SNPs offer useful marker resource for further genetic and genomic studies and will be helpful for Chinese fir breeding programs.
    Źródło:
    Dendrobiology; 2016, 76
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
    Autorzy:
    Wachowiak, W
    Baczkiewicz, A.
    Celinski, K.
    Prus-Glowacki, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/41357.pdf
    Data publikacji:
    2004
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Scotch pine
    Pinus sylvestris
    Pinus mugo
    dwarf pine
    hybridization
    DNA marker
    mtDNA
    trnV-rbcL region
    chloroplast
    DNA polymorphism
    Opis:
    Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
    Źródło:
    Dendrobiology; 2004, 51; 67-72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic structure of Picea abies populations growing on extreme sites as revealed by isoenzyme markers: a case study from Slovenia and Bosnia and Herzegovina
    Autorzy:
    Ballian, D
    Bogunic, F.
    Bozic, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/41007.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    plant population
    genetic structure
    extreme site
    isoenzyme marker
    genetic variation
    gene polymorphism
    forest tree
    Slovenia
    Bosnia and Herzegovina
    forest community
    Sphagno-Piceetum community
    Opis:
    Three populations of Norway spruce from ecologically extreme environments in Slovenia and Bosnia and Herzegovina were examined for genetic polymorphism. The spruces there grow in specific forest communities (Sphagno-Piceetum) which represent the remnants of the post-glacial vegetation. The aim of the study was to search for similarities in the genetic variation among populations adapted to such conditions. In total, 10 isoenzyme systems involving 16 gene loci were analysed. The results showed differences in genetic differentiation at loci Got-B, Skdh-A and 6-Pgdh-C between the two Slovenian populations and the Bosnian population, but also indicated an interestingly close relationship between the Slovenian population Pohorje and the Bosnian population Nišići.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic polymorphism of Polish strains of Gremmeniella abietina and Brunchorstia pinea var. cembrae
    Autorzy:
    Kraj, W
    Powiązania:
    https://bibliotekanauki.pl/articles/41508.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    coniferous tree
    plant pathology
    pine
    spruce
    fir
    juniper
    pathogen
    plant disease
    shoot blight
    canker
    branch
    stem
    Gremmeniella abietina
    Brunchorstia pinea var.cembrae
    needle
    genetic polymorphism
    Polish strain
    host preference
    polymerase chain reaction
    RFLP analysis
    Opis:
    Thirty-three type A strains of G. abietina from diseased shoots or needles of P. sylvestris, P. nigra and P. armandii and three strains of Brunchorstia pinea var. cembrae from P. mugo were isolated from four regions of Poland differing with respect to climatic conditions. Genetic polymorphism of the mitochondrial small subunit rRNA (mtSSU rRNA), ribosomal RNA fragment including ITS1, 5.8S and ITS2 and glyceraldehyde phosphate dehydrogenase (GPD) gene was examined by the PCR-RFLP method. Genetic distance was ascertained with respect to B. pinea var. cembrae strains from G. abietina isolated from the examinedpine species (average Nei coefficient 0.137). The smallest genetic distance occurred between the strain groups of G. abietina isolated from P. nigra and P. armandii (0.059) and P. nigra and P. sylvestris (0.061), whereas the highest occurred between the groups of strains deriving from P. armandii and P. sylvestris (0.096). The impact of geographic distance on genetic distance between groups of strains from individual regions has been shown. G. abietina strains originating from mountainous areas were more distanced genetically (on average 0.031) from populations from other regions (Nei genetic distance 0.023). The main factors influencing genetic differences of the pathogen were specificity with respect to the species of the host plant and climate conditions, whereas geographic distance had lesser significance.
    Źródło:
    Dendrobiology; 2009, 61; 13-21
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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