- Tytuł:
- Preliminary results on studies of in vivo and in vitro sexual reproduction of Salix viminalis L.
- Autorzy:
-
Zenkteler, M
Wojciechowicz, M.
Bagniewska-Zadworna, A.
Jezowski, S. - Powiązania:
- https://bibliotekanauki.pl/articles/41263.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polska Akademia Nauk. Instytut Dendrologii PAN
- Tematy:
-
embryonic development
Salix viminalis
willow
in vivo
sexual reproduction
in vitro
self-pollination
poplar - Opis:
- In vivo and in vitro self-pollination of whole pistils of some clones of Salix viminalis enabled to obtain mature seeds containing cotyledonary embryos which after the transfer to MS medium developed into wholly formed seedlings. Pollination in vitro of placentae led to abundant pollen germination and formation of tubes which occasionally they were entering the ovules through micropyle. Fertilized ovules normally developed into germinable seeds. Distant pollination of stigmas in vivo and in vitro with pollen grains of Populus tremula, P.tomentosa, P. lasiocarpa showed the ability of pollen to germinate and to form tubes several hours after pollination. Some tubes penetrated the styles but did not enter into the placenta. When placentae were directly pollinated than pollen germinated abundantly and occasionally pollen tubes were found entering the micropyle. Embryological analysis of those ovules performed 3-5 days after pollination demonstrated the presence of globular embryos with several endosperm nuclei. The technique of in vitro placental pollination works well for Salix viminalis and it could probably be applied to other Salix species.
- Źródło:
-
Dendrobiology; 2003, 50
1641-1307 - Pojawia się w:
- Dendrobiology
- Dostawca treści:
- Biblioteka Nauki
Artykuł