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    Wyświetlanie 1-6 z 6
    Tytuł:
    Micropropagation of Calycanthus fertilis
    Autorzy:
    Kulpa, D.
    Kubus, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41353.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    in vitro culture
    micropropagation
    Calycanthus fertilis
    multiplication
    plant growth regulator
    Opis:
    Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.
    Źródło:
    Dendrobiology; 2010, 64
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tree somatic embryogenesis in science and forestry
    Autorzy:
    Hazubska-Przybyl, T.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41255.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    tree
    somatic embryogenesis
    embryogenesis
    science
    forestry
    in vitro culture
    seedling
    somatic seedling
    forest management
    Opis:
    Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
    Źródło:
    Dendrobiology; 2016, 76
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro responses of various explants of Fagus sylvatica
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41768.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    European beech
    plant response
    explant
    Fagus sylvatica
    micropropagation
    culture condition
    tissue culture
    in vitro culture
    Opis:
    There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
    Źródło:
    Dendrobiology; 2015, 73
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro callus and shoot organogenesis from leaf and stem explants of Chamaedaphne calyculata
    Autorzy:
    Zrobek-Sokolnik, A.
    Dynowski, P.
    Holdynski, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/41437.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    in vitro culture
    callus
    shoot
    organogenesis
    leaf
    stem
    explant
    Chamaedaphne calyculata
    Ericaceae
    micropropagation
    plant species
    plant conservation
    Opis:
    Leatherleaf Chamaedaphne calyculata (L) Moench is a relict, rare and endangered species in Poland. There are no reports on the micropropagation of Chamaedaphne calyculata in the literature. Therefore, the aim of this study was to propose a propagation protocol for leatherleaf via indirect organogenesis using leaves and stems (internodal segments) derived from mature plants growing in a natural stand and from plants grown in vitro as explants. The medium developed by Anthony et al. (2004) with 100%, 50% and 25% salt concentrations, supplemented with IAA (5 and 10μM) and TDZ (5 and 10μM), was used for callus development and the induction of adventitious shoots. The media developed by Anthony et al. (2004) and Anderson (1980), both containing 10μM TDZ and 5μM IAA or 2.28μM zeatin, were used for adventitious shoot elongation. Secondary explants proved to be the most effective starting material for callus induction, the regeneration and elongation of adventitious shoots. The most supportive medium for callus induction and growth and the induction of adventitious shoots was the full medium proposed by Anthony et al. (2004) containing 5μM IAA and 10μM TDZ. Anderson’s (1980) medium containing 2.28μM zeatin delivered optimal results in the elongation of adventitious shoots of Ch. calyculata. Roots were cultivated on Anderson’s (1980) phytohormone-free medium. Approximately 65% of the plantlets survived after transfer to the sphagnum-peat and perlite mixture (3:1). The plants grew normally without any signs of morphological variation. This study makes the first ever attempt to propose an effective micropropagation protocol for Ch. calyculata.
    Źródło:
    Dendrobiology; 2014, 72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of aluminium and copper on the development of birch (Betula pendula Roth.) cultured in vitro and in vivo
    Autorzy:
    Bojarczuk, K
    Szczygiel, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41028.pdf
    Data publikacji:
    2004
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    environment pollution
    industrial pollution
    tree
    birch
    Betula pendula
    plant development
    soil pollution
    toxic metal
    aluminium
    copper
    in vivo culture
    in vitro culture
    Opis:
    Adventitious bud cultures were established by using buds of a selected birch clone (Betula pendula Roth.) resistant to industrial pollution. The Murashige and Skoog medium (1/2 and 1/4 MS) was used for multiplication and rooting of shoots. Aluminium was added to the medium, in the form of aluminium sulphate (50–100 mg Al dm–3), and birch culture was continued in vitro for over 12 months. The shoots developed on media with aluminium (Al+) proved to be more tolerant to aluminium and copper (added to the medium as nitrates or sulphates, at a concentration of 0.05–2.0 mM) during multiplication and rooting than those developed on media without aluminium (Al–). Rooted birch microcuttings obtained from cultures on media with aluminium (Al+) grew better in the soil from an unpolluted area (Zwierzyniec, Z) and from an area polluted by a phosphate fertilise factory (Luboń, L) than those from media without aluminium (Al–).
    Źródło:
    Dendrobiology; 2004, 51; 3-8
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Stryphnolobium japonicum
    Autorzy:
    Kobus, M
    Kulpa, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/41239.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Styphnolobium japonicum zob.Sophora japonica
    Sophora japonica
    Japanese pagoda tree
    pagoda tree zob.Japanese pagoda tree
    scholar zob.japanese pagoda tree
    Chinese scholar zob.Japanese pagoda tree
    micropropagation
    vegetation
    development
    in vitro culture
    old tree
    growth regulator
    primary regeneration
    shoot multiplication
    rooting
    Opis:
    Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
    Źródło:
    Dendrobiology; 2009, 61; 23-26
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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