- Tytuł:
- Biochemical characterizations of anther-derived and inhibitor-selected mutants with enhanced lysine in rice [Oryza sativa L.]
- Autorzy:
-
Schaeffer, G W
Sharpe, F.T.
Dadley, J.T. - Powiązania:
- https://bibliotekanauki.pl/articles/2044454.pdf
- Data publikacji:
- 1998
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
selection
doubled haploid
protein purification
in vitro
amino acid
lysine
grain quality
germ plasm
nutritional quality
suspension culture
gene isolation
endosperm
Oryza sativa
inhibitor
rice - Opis:
- The nutritional quality of cereals, including rice, would be improved by increasing the lysine content. Experiments were designed to test whether cell lines and plants could be recovered from in vitro selections. Mutants of rice recovered from inhibitory levels of lysine plus threonine and S(2-aminoethyl)cysteine from anther-derived calli were regenerated and characterized at the whole plant, cell, and enzyme level. These procedures produced plants with 15% higher lysine in milled grains than the starting cultivar and provide excellent materials for basic studies in cell selections, lysine biochemistry, stress-related biochemistry and gene isolations. A tissue culture line is now available that exports protein more efficiently than the wild type cells and provides materials for the study of mechanisms of expression of the recessive gene(s) conditioning the enhanced lysine phenotype.
- Źródło:
-
Journal of Applied Genetics; 1998, 39, 1; 37-48
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł