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    Wyświetlanie 1-9 z 9
    Tytuł:
    Measurement of the upper respiratory tract aerated space volume using the results of computed tomography
    Autorzy:
    Rybak, Adam
    Zając, Andrzej
    Kukwa, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/221767.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CT studies
    upper respiratory tract diagnostics
    quantitative measurement of upper respiratory tract aerated space volume
    Opis:
    The paper presents the possibilities of quantitative analysis of results obtained from CT examination of organs and anatomical structures of the upper respiratory tract. The presented results of the analysis were obtained using proprietary software developed in the MATLAB 2018b environment (Image Processing toolbox). The software enables to visualize the original results of CT scan and, after evaluating the visible structures, enables to select the area to be subjected to quantitative analysis. After the initial identification of an area of interest requiring detailed diagnostics, its volume and the surface areas of individual cross-sections are calculated in the area separated for examinations. A graphical presentation of the analysis results - the surface areas of selected cross-sections possible to visualize in two- and three-dimensional space - enables quick analysis of changes in the examined region.
    Źródło:
    Metrology and Measurement Systems; 2019, 26, 2; 387-401
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The perspective of immunoprophylaxis and selected immunological issues in the course of the turkey rhinotracheitis
    Autorzy:
    Smialek, M.
    Tykalowski, B.
    Stenzel, T.
    Koncicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/32005.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    perspective
    immunoprophylaxis
    turkey rhinotracheitis
    animal infection
    vaccination
    upper respiratory tract
    animal disease
    turkey
    Opis:
    This review article presents immunological issues in the course of the turkey rhinotracheitis (TRT) emphasizing local immunity mechanisms, both humoral and cell-mediated, in the upper respiratory system. Studies on the influence of the humoral immunity in the course of infection and vaccinations against TRT have revealed many times the absence of correlation between the titre of specific IgY anti-aMPV (avian Metapneumovirus) antibodies in the serum and in the upper respiratory washings and the immunity against the occurrence of the clinical form of the TRT. Considering the above, T cells are increasingly often regarded as the main factor involved in the upper respiratory immunity against the TRT. However, there have been just a few reports on the role of the T cells in the local immunity processes in the infection with aMPV in turkeys. Additionally, studies of the T-cell-associated immunity against the TRT have given ambiguous results. Immunoprophylaxis issues against the aMPV infections are a significant part of the work where the authors confront current vaccination programmes against the perspectives of use of the future vaccines against the TRT. Future vaccines should face the following criteria: absence of the risk of immunosuppressive effect and reversion of vaccine strains virulence, ease-of-use combined with the possibility of administration of the vaccine to the large numbers of turkeys. The leading role in future vaccination programs for birds against the TRT is likely to be played by the in ovo technique and the recombinant vaccines. Great hopes are also linked with the development of subunit vaccines against the aMPV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008
    Autorzy:
    Markowska-Daniel, I
    Urbaniak, K.
    Stepniewska, K.
    Pejsak, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/30384.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Polska
    pig
    respiratory tract
    bacteria
    isolation
    antibiotic susceptibility
    animal health
    productivity
    animal production
    Opis:
    Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 29-36
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough
    Autorzy:
    Kalinowski, M.
    Adaszek, L.
    Miloszowska, P.
    Skrzypczak, M.
    Zietek-Barszcz, A.
    Kutrzuba, J.
    Gradzki, Z.
    Winiarczyk, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31384.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    molecular analysis
    gene
    canine adenovirus 2
    isolation
    dog
    disease symptom
    cough
    upper respiratory tract infection
    Opis:
    The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
    Autorzy:
    Cymerys, J.
    Dzieciatkowski, T.
    Slonska, A.
    Bierla, J.
    Tucholska, A.
    Chmielewska, A.
    Golke, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    replication
    animal disease
    horse
    equine herpesvirus 1
    respiratory tract infection
    neurological disorder
    abortion
    neuron
    Opis:
    Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anatomical and functional assessment of patency of the upper respiratory tract in selected respiratory disorders - Part 2
    Autorzy:
    Zając, Andrzej
    Kukwa, Andrzej
    Barański, Robert
    Nitkiewicz, Szymon
    Zomkowska, Edyta
    Rybak, Adam
    Powiązania:
    https://bibliotekanauki.pl/articles/2173877.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    optical diagnostic in otolaryngology
    upper respiratory tract diagnostics
    otolaryngology
    spirometer
    Fourier transform
    wavelet transform
    quantitative parameters of the respiratory cycle
    Opis:
    This article presents selected physical diagnostic methods used in otorhinolaryngology and results of their application. In addition to the applications of methods using the capabilities of selective sensors, selected methods of hybrid diagnostics were also presented - for assessment of parameters of respiratory processes, with polysomnography as an example of using both typical diagnostic methods dedicated to otolaryngology, as well as standard EEG and ECG methods. It has been shown that in some special cases of respiratory disorders, measurements of the air flow in the respiratory tract can be supplemented with pressure measurements in selected positions within the airways. The presented optical methods and diagnostic systems are very often used in the diagnosis of diseases not specific for otolaryngology occurring in the area of the head and neck. The presented material is the second part of the study discussing both standard and widely used diagnostic methods. All presented methods are dedicated to otolaryngology. This text is a continuation of the material published in No 4 of 2021 [1].
    Źródło:
    Metrology and Measurement Systems; 2022, 29, 3; 429--454
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anatomical and functional assessment of patency of the upper respiratory tract in selected respiratory disorders - Part 1
    Autorzy:
    Kukwa, Andrzej
    Zając, Andrzej
    Barański, Robert
    Nitkiewicz, Szymon
    Kukwa, Wojciech
    Zomkowska, Edyta
    Rybak, Adam
    Powiązania:
    https://bibliotekanauki.pl/articles/2052154.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    optical diagnostic in otolaryngology
    upper respiratory tract diagnostics
    otolaryngology
    spirometer
    Fourier transform
    wavelet transform
    quantitative parameters of the respiratory cycle
    Opis:
    The rapidly developing measurement techniques and emerging new physical methods are frequently used in otolaryngological diagnostics. A wide range of applied diagnostic methods constituted the basis for the review study aimed at presenting selected modern diagnostic methods and achieved diagnostic results to a wider group of users. In this part, the methods based on measuring the respiratory parameters of patients were analysed. Respiration is the most important and necessary action to support life and its effective duration. It is an actual gas exchange in the respiratory system consisting of removing CO2 and supplying O2. Gas exchange occurs in the alveoli, and an efficient respiratory tract allows for effective ventilation. The disruption in the work of the respiratory system leads to measurable disturbances in blood saturation and, consequently, hypoxia. Frequent, even short-term, recurrent hypoxia in any part of the body leads to multiple complications. This process is largely related to its duration and the processes that accompany it. The causes of hypoxia resulting from impaired patency of the respiratory tract and/or the absence of neuronal respiratory drive can be divided into the following groups depending on the cause: peripheral, central and/or of mixed origin. Causes of the peripheral form of these disorders are largely due to the impaired patency of the upper and/or lower respiratory tract. Therefore, early diagnosis and location of these disorders can be considered reversible and not a cause of complications. Slow, gradually increasing obstruction of the upper respiratory tract (URT) is not noticeable and becomes a slow killer. Hypoxic individuals in a large percentage of cases have a shorter life expectancy and, above all, deal with the consequences of hypoxia much sooner.
    Źródło:
    Metrology and Measurement Systems; 2021, 28, 4; 813-836
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Susceptibility testing of Aspergillus niger strains isolated from poultry to antifungal drugs - a comparative study of the disk diffusion, broth microdilution (M 38-A) and Etest methods
    Autorzy:
    Tokarzewski, S.
    Ziolkowska, G.
    Nowakiewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/32075.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    susceptibility
    Aspergillus niger
    isolation
    poultry
    antifungal drug
    disk diffusion
    broth microdilution method
    disc-diffusion method
    Etest procedure
    aspergillosis
    fungal infection
    respiratory tract
    Opis:
    The aim of this study was to determine the sensitivity of Aspergillus niger strains isolated from birds to available antifungal drugs using different in vitro assays - classical disk diffusion, Etest® and broth microdilution NCCLS/CLSI M 38-A. The study material consisted of about 2.000 swabs and samples from different species of birds. A. niger (n=10) was accounted for 6.81% of the total pool of strains isolated. Determinations were made for 13 antifungal drugs using the disk diffusion method. The A. niger exhibited high susceptibility to enilconazole, terbinafine, voriconazole, tioconazole and ketoconazole, low susceptibility to clotrimazole, miconazole and nystatin, and resistance to amphotericin B, itraconazole, pimaricin, fluconazole and 5-fluorocytosine. Minimum inhibitory concentration (MIC) was determined for 9 antifungal drugs using the micromethod of duplicate serial dilutions in a liquid medium. A. niger strains were most susceptible to enilconazole and voriconazole. MIC ranged from 0.0625 to 0.5 μg/ml for enilconazole, with MIC90-0.5 μg/ml and MIC50-0.125 μg/ml. The corresponding values for voriconazole were 0.25-1 μg/ml, 1 μg/ml and 0.5 μg/ml. MIC for amphotericin B and terbinafine ranged from 0.5 to 4 μg/ml, while the values for the remaining drugs were highly varied. MIC was measured by the gradient diffusion method using Etest® for 5 antifungal drugs: amphotericin B, fluconazole, itraconazole, ketoconazole and voriconazole. By far the highest susceptibility was obtained in the case of voriconazole, with MIC ranging from 0.0625 to 1 μg/ml. MIC for amphotericin B ranged from 0.25 to 4 μg/ml, for itraconazole and ketoconazole ranging from 0.5 to 16 μg/ml. Methods available for this purpose are not always applicable in field conditions. The present results indicate that the Etest® technique, due to its high percentage of agreement with the M 38-A microdilution method, should find application in medical and veterinary practice.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Laboratory diagnosis of mycoplasma infection in young cattle
    Autorzy:
    Gabinaitiene, A.
    Siugzdaite, J.
    Zilinskas, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/30791.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    mucous membrane
    Mycoplasma bovis
    Mycoplasma dispar
    animal infection
    antibody
    bacteriology
    cattle
    diagnosis
    histopathology
    laboratory diagnosis
    mycoplasma
    respiratory tract
    serology
    young animal
    isolation
    identification
    blood sample
    blood collecting
    Opis:
    The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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