Temat: real-time detection - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:: Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:: https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:: Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Real-Time Market Abuse Detection with a Stochastic Parameter Model
Autorzy:: Cholewiński, Radosław
Powiązania:: https://bibliotekanauki.pl/articles/483319.pdf
Data publikacji:: 2009
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Market abuse detection
insider trading
real-time analysis
timevarying parameters
uni- and bivariate GARCH processes
Opis:: This paper develops a new model of market abuse detection in real time. Market abuse is detected, as Minenna (2003) proposed, on the basis of prediction intervals. The model structure is based on the discrete-time, extended market model introduced by Monteiro, Zaman, Leitterstorf (2007) to analyze the market cleanliness. Parameters of the expected return equation are assumed, however, to be time-varying and estimated under the state-space framework using the extended Kalman filter postulated by Chou, Engle, Kane (1992) to capture the GARCH effect in returns. QML estimation is performed on intraday data; its utilization is proposed as an alternative to the continuous time modeling by Minenna (2003). This framework is generalized to the bivariate case which enables the analysis of daily open/close data. The paper also extends procedures of the statistical verification of the estimated state-space model to include the uncertainty arising from time-invariant parameters.
Źródło:: Central European Journal of Economic Modelling and Econometrics; 2009, 1, 3; 261-284
2080-0886
2080-119X
Pojawia się w:: Central European Journal of Economic Modelling and Econometrics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR
Autorzy:: Trzewik, A.
Nowak, K.J.
Orlikowska, T.
Powiązania:: https://bibliotekanauki.pl/articles/66480.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method
Opis:: Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
Źródło:: Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Sensitive and specific detection of Xanthomonas hortorum pv. pelargonii in geranium by real-time PCR
Autorzy:: Farahani, A.S.
Taghavi, S.M.
Powiązania:: https://bibliotekanauki.pl/articles/66354.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: detection
bacterial blight
Xanthomonas hortorum pv.pelargonii geranium
real-time polymerase chain reaction
pathogen
Botrytis cinerea
Puccinia pelargonii-zonalis
Pythium
Rhodococcus fascians
Ralstonia solanacearum
Opis:: Xanthomonas hortorum pv. pelargonii is the causal agent of bacterial blight of geranium. A specific and rapid real-time PCR assay for detecting the bacterium in plant was developed in this study. We compared sensitivity of conventional and real-time PCR for detection of the pathogen in inoculated plants. For application to disease management programs, PCR amplification must be able to detect latent infections of asymptomatic geraniums. Our results displayed that conventional PCR lacks sufficient sensitivity to be used as diagnostic tools for detection of X. hortorum pv. pelargonii in latent infections. On the other hand, real-time PCR is suitable method for detection of latent infection of the bacterium in planting materials and can be considered in management programs. The ability for accurate and reliable detection of X. hortorum pv. pelargonii in asymptomatic tissue is a significant step in setting up “pathogen free” certification programs for bacterial blight of geranium.
Źródło:: Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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