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Wyszukujesz frazę "immunohistochemistry" wg kryterium: Temat

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Wyświetlanie 1-15 z 17
Tytuł:
The inflammatory reaction and the expression of pro- and anti-inflammatory cytokines to a novel polyester-polyurethane aortic prosthesis evaluated in dog sat 6 and 12 months post-implantation
Autorzy:
Mālniece, A.
Auzāns, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087649.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polyester
polyurethane
inflammation
immunohistochemistry
aortic prosthesis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 655-664
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of follicle-stimulating hormone receptor (FSHR), protein kinase B-2 (AKT2) and adapter protein with PH domain, PTB domain, and leucine zipper (APPL1) in pig ovaries
Autorzy:
Ning Zhou
Ning Wang
Xiang Qin
Qian Liu
Juhua Wang
Hui Dong
Jie Zhou
Fugui Fang
Powiązania:
https://bibliotekanauki.pl/articles/2087861.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pigs
immunohistochemistry
FSHR
AKT2
APPL1
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 661-667
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical properties of motoneurons supplying the trapezius muscle in the rat
Autorzy:
Dudek, A.
Sienkiewicz, W.
Marczak, M.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/30477.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
immunohistochemical property
motoneuron
trapezius muscle
rat
tracing
immunohistochemistry
Opis:
Combined retrograde tracing (using fluorescent tracer Fast blue) and double-labelling immunofluorescence were used to study the distribution and immunohistochemical characteristics of neurons projecting to the trapezius muscle in mature male rats (n=9). As revealed by retrograde tracing, Fast blue-positive (FB+) neurons were located within the ambiguous nucleus and accessory nucleus of the grey matter of the spinal cord. Immunohistochemistry revealed that nearly all the neurons were cholinergic in nature [choline acetyltransferase (ChAT)-positive]. Retrogradely labelled neurons displayed also immunoreactivities to calcitonin gene-related peptide (CGRP; approximately 60% of FB+ neurons), nitric oxide synthase (NOS; 50%), substance P (SP; 35%), Leu5-Enkephalin (LEnk; 10%) and vasoactive intestinal polypeptide (VIP; 5%). The analysis of double-stained tissue sections revealed that all CGRP-, VIP- and LEnk-immunoreactive FB+ perikarya were simultaneously ChAT-positive. The vast majority of the neurons expressing SP- or NOS-immunoreactivity were also cholinergic in nature; however, solitary somata were ChAT-negative. FB+ perikarya were surrounded by numerous varicose nerve fibres (often forming basket-like structures) immunoreactive to LEnk or SP. They were also associated with some CGRP-, NOS- and neuropeptide Y-positive nerve terminals.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enteric neuronal development in canine small intestine – an immunohistochemical study
Autorzy:
Serzysko, T.
Skwarek, A.
Chudziak, E.
Malina, M.
Kaleczyc, J.
Sienkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087175.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dog
fetus
small intestine
enteric nervous system
development
neurons
immunohistochemistry
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 293-301
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution and chemical coding of urinary bladder apex-projecting neurons in aorticorenal and testicular ganglia of the male pig
Autorzy:
Pidsudko, Z.
Listowska, Ż.
Franke-Radowiecka, A.
Klimczuk, M.
Załęcki, M.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087510.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
prevertebral ganglia
urinary bladder apex
immunohistochemistry
retrogradetracing
neuropeptides
male pig
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 427-430
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison
Autorzy:
Mozel, S.
Szymańczyk, S.
Krzysiak, M.
Puzio, I.
Zacharko-Siembida, A.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087293.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
glucagon-like peptide-1
glucagon
insulin
somatostatin
immunohistochemistry
pancreas
European bison
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 333-340
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution and amount of cathepsin B in gentamicin-induced acute kidney injury in rats
Autorzy:
Svara, T
Pogacnik, M.
Juntes, P.
Powiązania:
https://bibliotekanauki.pl/articles/30766.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rat
kidney
cathepsin B
gentamicin
distribution
immunohistochemistry
vacuolar degeneration
histopathology
statistical method
Opis:
The aim of our study was to investigate how the distribution and amount of cathepsin B change during acute kidney injury. The research was done on a rat model of acute kidney injury that was induced by nephrotoxic antibiotic gentamicin. Gentamicin was injected at a dose of 40 mg/kg body weight (the first treated group) and 80 mg/kg body weight (the second treated group) for 14 days. Control groups received injections of physiological saline only. One day after the last injection, animals were euthanized, dissected and kidney samples were taken and fixed in 10% buffered formalin. Tissue sections were stained with haematoxylin and eosin, periodic Acid Schiff (PAS) and Oil-red-O. Immunohistochemistry was used for the demonstration of cathepsin B. Vacuolar degeneration of the proximal convoluted tubules was the most prominent pathologic lesion found in the first treated group, while necrosis prevailed in the second treated group in the same localisation. In both treated groups significantly weaker immunohistochemical reaction for cathepsin B was noticed in the proximal convoluted tubules in comparison to the control groups (P < 0.05). The decrease of positive reaction was the largest in the proximal convoluted tubules of the outer renal cortex. Stronger positive reaction for cathepsin B, although not statistically significant, was found in the proximal straight tubules (P > 0.05), as well. However, more numerous cathepsin B-positive large granules appeared in the proximal straight tubules of the second treated group then in the second control group (P < 0.05). We can conclude that the amount of cathepsin B in the affected proximal convoluted tubules significantly decreases along the increased severity of the histopathological lesions of the proximal convoluted tubuls, the amount of enzyme in the well preserved proximal straight tubules increases and more cathepsin B-positive large granules appear in the cytoplasm.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 75-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas
Autorzy:
Sokolowska, J.
Micun, J.
Zabielska, K.
Malicka, E.
Lechowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/31685.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
immunohistochemistry
expression
immunoglobulin
dog
B cell
lymphoma
tumour
neoplastic cell
immunoglobulin light chain
cancer
Opis:
Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of λ light chains and κ light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (sκ/sλ). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (cκ/cλ). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM+ and IgG+ phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin λ light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa’s ganglion) of the pig
Autorzy:
Dudek, A.
Sienkiewicz, W.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/31779.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
immunohistochemistry
neuron
vestibular ganglion
pig
Scarpa’s ganglion zob.vestibular ganglion
nerve fibre
Opis:
The study was carried out on three 4-month old female pigs. All the animals were deeply anesthetized and transcardially perfused with 4% buffered paraformaldehyde (pH 7.4). Vestibular ganglia (VG) were collected and processed for double-labelling immunofluorescence method. The preparations were examined under the Zeiss LSM 710 confocal microscope equipped with adequate filter blocks. Neurons forming VG were round or oval in shape with a round nucleus in the center. The majority of them (58%) were medium (M) (31-50 μm in diameter) while 28 % and 14% were small (S) (up to 30 μm in diameter) or large (L) (above 50 μm in diameter) in size, respectively. Double-labeling immunofluorescence revealed that VG neurons stained for CGRP (approx. 81%; among them 70.5%, 26.2% and 3.3% were M, S and L in size, respectively), VACHT (57%; 63% M, 24% S, 13% L), Met-Enk (25%; 60% M, 12% S, 28% L), VIP (20%; 88% M, 6% S, L), NPY (15%; 67% M, 20% S, 13% L), GAL (15%; 74% M, 21% S, 5% L), SP (12%; 69% M, 25% S, 6% L) and NOS-positive (12%; 50% S, 50% M). The most abundant populations of intraganglionic nerve fibers were those which stained for CGRP or Met-Enk, whereas only single SP- or NOS-positive nerve terminals were observed.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of resiniferatoxin on the chemical coding of neurons in dorsal root ganglia supplying the urinary bladder in the female pig
Autorzy:
Bossowska, A.
Majewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/30452.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
resiniferatoxin
chemical coding
neuron
dorsal root ganglion
urinary bladder
female
pig
sensory innervation
immunohistochemistry
neuropeptide
Opis:
Although resiniferatoxin (RTX) becomes more often used in experimental therapies of sensory system disorders, so far there is no data concerning the influence of RTX on the chemical coding of neurons in dorsal root ganglia (DRG) supplying the urinary bladder in the pig, an animal species considered as a reliable animal model for investigation dealing with human lower urinary tract disorders. Retrograde tracer Fast Blue (FB) was injected into the wall of the right half of the urinary bladder in six juvenile female pigs, and three weeks later, bladder instillation of RTX (500 nmol per animal) was carried out in all the animals. After a week, DRGs were harvested from all the pigs and the neurochemical characterization of FB+ neurons was performed using routine single-immunofluorescence labeling technique on 10-μm-thick cryostat sections. RTX instillation resulted in a distinct decrease in the numbers of FB+ cells containing calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), somatostatin (SOM) and calbindin (CB) when compared with those found in the healthy animals (18% vs. 36%, 1% vs. 6%, 0.8% vs. 4% and 0.5% vs. 3%, respectively), and an increase in the number of pituitary adenylate cyclase-activating polypeptide (PACAP)- and galanin (GAL)-immunoreactive (IR) nerve cells (51% vs. 26% and 47% vs. 6.5%). The results obtained suggest that RTX could be taken into consideration when the neuroactive agents are planned to be used in experimental therapies of selected neurogenic bladder illnesses.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conantokin G-induced changes in the chemical coding of dorsal root ganglion neurons supplying the porcine urinary bladder
Autorzy:
Bossowska, A.
Majewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/31315.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conantokin G
chemical coding
urinary bladder
dorsal root ganglion
neuron
sensory innervation
immunohistochemistry
neuropeptide
pig
Opis:
Conantokin G (CTG), isolated from the venom of the marine cone snail Conus geographus, is an antagonist of N-methyl-d-aspartate receptors (NMDARs), the activation of which, especially those located on the central afferent terminals and dorsal horn neurons, leads to hypersensitivity and pain. Thus, CTG blocking of NMDARs, has an antinociceptive effect, particularly in the case of neurogenic pain treatment. As many urinary bladder disorders are caused by hyperactivity of sensory bladder innervation, it seems useful to estimate the influence of CTG on the plasticity of sensory neurons supplying the organ. Retrograde tracer Fast Blue (FB) was injected into the urinary bladder wall of six juvenile female pigs. Three weeks later, intramural bladder injections of CTG (120 μg per animal) were carried out in all animals. After a week, dorsal root ganglia of interest were harvested from all animals and neurochemical characterization of FB+ neurons was performed using a routine double-immunofluorescence labeling technique on 10-μm-thick cryostat sections. CTG injections led to a significant decrease in the number of FB+ neurons containing substance P (SP), pituitary adenylate cyclase activating polypeptide (PACAP), somatostatin (SOM), calbindin (CB) and nitric oxide synthase (NOS) when compared with healthy animals (20% vs. 45%, 13% vs. 26%, 1.3% vs. 3%, 1.2 vs. 4% and 0.9% vs. 6% respectively) and to an increase in the number of cells immunolabelled for galanin (GAL, 39% vs. 6.5%). These data demonstrated that CTG changed the chemical coding of bladder sensory neurons, thus indicating that CTG could eventually be used in the therapy of selected neurogenic bladder illnesses.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prognostic significance of p53 protein accumulation in cancer cells obtained from selected group of patients with sporadic colorectal cancer
Autorzy:
Paluszkiewicz, P
Karski, J.
Berbec, H.
Pawlowska-Wakowicz, B.
Cybulski, M.
Karski, M.
Paszkowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2043897.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
patient
cancer cell
monoclonal antibody
p53 protein
cancer prognosis
immunohistochemistry
sporadic colorectal cancer
accumulation
colorectal cancer
Źródło:
Journal of Applied Genetics; 1999, 40, 2; 135-144
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The expression of androgen receptor in neurons of the anterior pelvic ganglion and celiac-superior mesenteric ganglion in the male pig
Autorzy:
Kaleczyc, J.
Kasica-Jarosz, N.
Pidsudko, Z.
Przyborowska, A.
Sienkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087598.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
androgen receptor
neurons
anterior pelvic ganglion
celiac-superior mesenteric ganglion
quantitative real-time PCR
immunohistochemistry
male pig
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 151-155
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative studies on bone structure in dairy cows with different feeding conditions
Autorzy:
Pilmane, M
Zitare, I.
Jemeljanovs, A.
Powiązania:
https://bibliotekanauki.pl/articles/30482.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dairy cattle
cow
animal feeding
bone structure
feeding condition
histomorphometry
immunohistochemistry
bone
diet
rapeseed
dynamic structure
feed component
Opis:
The bone belongs to the dynamic tissues and its structure in domestic cows is still not completely understood in correlation to the impact of different food components. The aim of our work was a histomorphometrical and immunohistochemical research on bone morphology and factors influencing it in healthy dairy cows fed with self-produced grain and with rapeseed cakes. The bone of self-produced grain-fed cows demonstrated statistically significant difference in the number of osteocytes from the bone of rapeseed cakes-fed cows. The rapeseed cakes-fed cows didn't show any bone cell positive for BMP2/4, while FGFR1 increased significantly in their supportive tissues. The number of bFGF- and apoptosis-containing structures varied in cows of both groups. MMP2 expression showed statistically significant difference between both animals' groups with domination in bone of cows fed with self-produced grain. Defensin-, osteopontin- and osteocalcin-contain- ing cells showed tendency to increase in bone of cows on rapeseed cakes diet. Conclusions. The rapeseed-fed cow's long bones demonstrate significant decrease of osteocytes per mm2 and selective increase of FGFR1, suggesting the (compensatory) growth stimulation in supportive tissue. The statistically significant selective absence of MMP2 with a slight tendency of increase in osteopontin and osteocalcin in rapeseed-fed cow's long bones indicates the persistence of seemingly still compensated qualitative changes in bone (beginning of disturbances in mineralization, metabolism etc.) proved also by a slight increase of the bone antimicrobial peptide.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 89-95
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Density of tumor-associated macrophages (TAMs) and expression of their growth factor receptor MCSF-R and CD14 in canine mammary adenocarcinomas of various grade of malignancy and metastasis
Autorzy:
Krol, M.
Pawlowski, K.
Majchrzak, K.
Dolka, I.
Abramowicz, A.
Szyszko, K.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/32107.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adenocarcinoma
canine adenocarcinoma
density
dog
expression
growth factor
immunohistochemistry
immunology
malignancy
mammary adenocarcinoma
metastasis
tissue sample
tumour microenvironment
tumour-associated macrophage
Opis:
Several years ago, the presence of macrophages in the tumor microenvironment was thought to be an inflammatory response to kill the cancer cells. Now, this is clear that the inflammatory cells that exit blood vessels and migrate to the tumor tissue play an important role in cancer progression. Various cells present in the tumor microenvironment enhance cancer growth and invasiveness by secretion of tumor-enhancing products. That is why tumors should not be treated as only aggregates of cancer cells but as separate structures. Macrophages form a major component of the inflammatory infiltration in tumors, where they are termed tumor-associated macrophages (TAMs). To the best of our knowledge, up-to-date there were no studies on tumor associated macrophages and the role of the tumor microenvironment in tumor invasion/metastasis in dogs. This is the first study performed to asses if the number of TAMs and expression of MCSF-R (macrophages colony stimulating factor receptor) and CD14 (LPS co-receptor) are associated with the grade of tumor malignancy and its ability to metastasize. We have performed immunohistochemical analysis of 50 canine mammary adenocarcinomas of various grade of malignancy (1st, 2nd, 3rd) and tumors that gave local or distant metastases. The results indicate that in dogs, similarly to humans and mice, the number of tumor associated macrophages is related to the cancer ability to metastasize. Our results also indicate that the expression of MCSF-R and, what is particularly new finding, CD14 is associated with tumor malignancy and its ability to metastasize. Hence, these molecules play a role in tumor progression, metastasis and microenvironment interactions. These results show that in dogs we should treat the tumor as a whole organ rather than just try to eliminate the cancer cells.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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