- Tytuł:
- Laccase immobilisation on mesostructured silicas
- Autorzy:
-
Bryjak, J.
Szymańska, K.
Jarzębski, A.B. - Powiązania:
- https://bibliotekanauki.pl/articles/185475.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
laccase
Immobilization
mesostructured enzyme carrier
thermal stability
lakaza
unieruchomienie
enzym
stabilność termiczna - Opis:
- Extracellular laccase produced by the wood-rotting fungus Cerrena unicolor was immobilised covalently on the mesostructured siliceous foam (MCF) and three hexagonally ordered mesoporous silicas (SBA-15) with different pore sizes. The enzyme was attached covalently via glutaraldehyde (GLA) or by simple adsorption and additionally crosslinked with GLA. The experiments indicated that laccase bound by covalent attachment remains very active and stable. The best biocatalysts were MCF and SBA-15 with Si–F moieties on their surface. Thermal inactivation of immobilised and native laccase at 80°[degrees]C showed a biphasic-type activity decay, that could be modelled with 3parameter isoenzyme model. It appeared that immobilisation did not significantly change the mechanism of activity loss but stabilised a fraction of a stable isoform. Examination of time needed for 90% initial activity loss revealed that immobilisation prolonged that time from 8 min (native enzyme) up to 155 min (SBA-15SF).
- Źródło:
-
Chemical and Process Engineering; 2012, 33, 4; 611-620
0208-6425
2300-1925 - Pojawia się w:
- Chemical and Process Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł