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Wyszukujesz frazę "cell culture" wg kryterium: Temat


Tytuł:
Comparative analysis of cat bone marrow and adipose tissue cell cultures
Autorzy:
Mazurkevych, A.
Malyuk, M.
Kovpak, V.
Kovpak, O.
Kharkevych, Y.
Jakubczak, A.
Gryzinska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087658.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stem cells
bone marrow cell culture
adipose tissue cell culture
cytogenetic assays
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 549-557
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biomimetic alginate/perfluorocarbon microcapsules - tthe effect on in vitro metabolic activity and long-term cell culture
Autorzy:
Stefanek, Agata
Kulikowska-Darłak, Aleksandra
Bogaj, Karolina
Nowak, Aleksandra
Dembska, Joanna
Ciach, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/2173417.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
alginian
mikrokapsułki
hodowla komórkowa
perfluorowęglowodory
metabolizm
alginate
microcapsules
cell culture
perfluorocarbons
metabolism
Opis:
Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.
Źródło:
Chemical and Process Engineering; 2022, 43, 1; 81--95
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological and biochemical characteristics of Spodoptera exigua nuclear polyhedrosis virus [SeMNPV] [Baculoviridae] stored for a long time at different formulations
Autorzy:
Jakubowska, A.
Ziemnicka, J.
Powiązania:
https://bibliotekanauki.pl/articles/66562.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insect population
Baculoviridae
commercial product
baculovirus
Spodoptera exigua
storage stability
cell culture
nuclear polyhedrosis virus
Źródło:
Journal of Plant Protection Research; 2003, 43, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cryptic rearrangements of chromosome 12 in testicular germ cell tumors with or without the specific i[12p] marker
Autorzy:
Grygalewicz, B
Pienkowska-Grela, B.
Woroniecka, R.
Powiązania:
https://bibliotekanauki.pl/articles/2043149.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aberration
in situ
isochromosome
cytogenetic analysis
microdissection
testicular germ cell tumour
karyotype
cell culture
nonseminoma
fluorescence
hybridization
seminoma
chromosome 12
DNA
Źródło:
Journal of Applied Genetics; 2000, 41, 2; 123-131
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of in vitro culture conditions for obtaining flax (Linum usitatissimum L. cv. Modran) cell suspension culture
Autorzy:
Seta-Koselska, A.
Skorzynska-Polit, E.
Powiązania:
https://bibliotekanauki.pl/articles/81015.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Linum usitatissimum
flax
in vitro culture
alpha-linolenic acid
phytochemical
lignan
callus culture
cell suspension culture
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Properties of polyurethane fibrous materials produced by solution blow spinning
Autorzy:
Łopianiak, Iwona
Wojasiński, Michał
Butruk-Raszeja, Beata
Powiązania:
https://bibliotekanauki.pl/articles/2034056.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nano- and microfibres
3D cell culture
solution blow spinning
polyurethanes
scaffolds
nano- i mikrowłókna
hodowla komórek 3D
wirowanie z rozdmuchiwaniem roztworu
poliuretany
rusztowania
Opis:
The study aimed to produce nano- and microfibrous materials from polyurethane (ChronoFlex®C75A/ C75D in 1,1,1,3,3,3–hexafluoro–2–propanol) by solution blow spinning. Experiments were carried out in order to determine the impact of solution blow spinning parameters on fibre diameter and quality of produced materials. The following properties of produced fibre scaffolds were investigated: fibre size, porosity and pore size, wettability, and mechanical properties. The results confirmed that produced nano- and microfibrous materials could be potentially used as scaffolds in three-dimensional cell and tissue cultures.
Źródło:
Chemical and Process Engineering; 2020, 41, 4; 267-276
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation
Autorzy:
Pilarek, M.
Godlewska, K.
Kuźmińska, A.
Wojasiński, M.
Dąbkowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/184988.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wave-induced agitation
small-scale animal cell culture
CP5 chondrocytes
fibrousbased
scaffold
single-use bioreactor
wzbudzanie falowe
hodowla komórek zwierzęcych
Chondrocyty CP5
szafot
bioreaktor
Opis:
One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.
Źródło:
Chemical and Process Engineering; 2017, 38, 2; 321-330
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects
Autorzy:
Pilarek, M.
Powiązania:
https://bibliotekanauki.pl/articles/185200.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ciecz perfluorochemiczna
fluorowęgiel
gaz ciekły
rozpuszczalność
hybrydowy system hodowli
liquid perfluorochemical
fluorocarbon
liquid gas carrier
solubility
cell/tissue culture
hybrid culture system
Opis:
Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O2 and CO2) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo- and thermostability, extremely low surface tension, simultaneous hydro- and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C–F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.
Źródło:
Chemical and Process Engineering; 2014, 35, 4; 463-487
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier
Autorzy:
Pilarek, M.
Dąbkowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/185054.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
liquid perfluorochemical (fluorocarbon)
liquid gas carrier
mathematical modelling
cell/tissue culture
hybrid culture system
ciecz perfluorochemiczna (fluorowęglowa)
gaz ciekły
modelowanie matematyczne
hybrydowy system hodowli
Opis:
A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.
Źródło:
Chemical and Process Engineering; 2016, 37, 1; 149-158
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of Arabidopsis thaliana (col-0) plants and cell suspension cultures responses to Alternaria brassicicola infection
Autorzy:
Rousseau, K.
Macioszek, V.K.
Kononowicz, A.K.
Powiązania:
https://bibliotekanauki.pl/articles/81100.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Alternaria brassicicola
necrotrophic pathogen
fungal pathogen
cruciferous plant
black spot disease
Arabidopsis thaliana
cell suspension
cell suspension culture
fungal infection
reactive oxygen species
hydrogen peroxide
superoxide anion
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro cultures of animal and human cells
Autorzy:
Slomski, R.
Nowak, A.
Hryhorowicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/951215.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vitro culture
tissue culture
animal cell
human cell
homeostasis
fluorescence in situ hybridization
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Recent advances and novelties in the thin cell layer-based plant biotechnology – a mini-review
Autorzy:
Da Silva, J.A.T.
Dobranszki, J.
Powiązania:
https://bibliotekanauki.pl/articles/80834.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
thin cell layer
fruit
vegetable
in vitro culture
micropropagation
organogenesis
somatic embryogenesis
plant biotechnology
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)
Autorzy:
Pindel, A.
Wiszniewska, A.
Piwowarczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/81216.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant protoplast
isolation
cell wall
enzymatic method
plant regeneration
protoplast culture
memory
Pojnar Edward
biography
scientific activity
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rice improvement, involving altered flower structure more suitable to cross-pollination, using in vitro culture in combination with mutagenesis
Autorzy:
Min, S K
Powiązania:
https://bibliotekanauki.pl/articles/2048292.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
japonica rice
radiation
China
mutagenesis
mutant
anther culture
in vitro
cross-pollination
hybridization
indica rice
somatic cell
Oryza sativa
rice
Opis:
Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 151-162
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of isolation stress on the (re)organization of cell walls in protoplasts of in vitro recalcitrant plants
Autorzy:
Wiszniewska, A.
Piwowarczyk, B.
Pindel, A.
Powiązania:
https://bibliotekanauki.pl/articles/80981.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
protoplast culture
cell wall
regeneration
cellulose
arabinogalactan protein
isolation stress
reorganization
recalcitrant plant
plant species
yellow lupin
grass pea
hyacinth
asparagus
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł

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