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    Wyświetlanie 1-8 z 8
    Tytuł:
    Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
    Autorzy:
    Maslanka, T
    Jaroszewski, J.J.
    Markiewicz, W.
    Jabubowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/31556.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    peripheral blood
    T cell
    calf
    CD4plus T cell
    CD8plus T cell
    apoptosis
    meloxicam
    flunixin meglumine
    lymphocyte
    antiinflammatory drug
    Opis:
    The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lymphocyte subpopulations and apoptosis of immune cells in rabbits experimentally infected with a strain of the RHD virus having a variable haemagglutination capacity
    Autorzy:
    Niedzwiedzka-Rystwej, P.
    Deptula, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31022.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    lymphocyte subpopulation
    apoptosis
    immune cell
    rabbit
    experimental infection
    RHD virus
    hemagglutination capacity
    granulocyte
    T cell
    B cell
    Opis:
    The paper describes the immunological response in the matter of percentage of T cells (receptor CD5+) and subpopulations (Th with receptor CD4+, Tc/Ts with receptor CD8+, T with receptor CD25+) and B cells with receptor CD19+, as well as the percentage of apoptotic granulocytes and lymphocytes, in rabbits experimentally infected with the Hagenow strain of the RHD virus. The material chosen for the experiment is special, as among all strains of RHD virus, there are only two strains which carry the variable haemagglutination capacity of red cells. The results of the study show that the Hagenow strain gives an untypical picture of T and B lymphocytes, whereas the results in inducing apoptosis seems to corespond with previous data, confirming the inclusion of apoptosis from 4 h p.i. and the intensity of the phenomenon being higher in granulocytes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of CD4plus T cell epitopes from Staphylococcus aureus secretome using immunoinformatic prediction and molecular docking
    Autorzy:
    Francis, D.
    Kumar, A.
    Chittalakkottu, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096359.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CD4+
    T cell
    epitope prediction
    secretory proteins
    Staphylococcus aureus
    peptide vaccine
    molecular docking
    Opis:
    One major reason for the lack of clinical success of Staphylococcus aureus vaccine candidates is the inability of the antigens to develop a CD4+ T cell-mediated immune response. Hence, it is important to identify CD4+ T cell antigens from S. aureus. CD4+ T cells are activated following the presentation of epitopes derived from exogenous proteins on HLA class II molecules. Fifty-nine secretory proteins of S. aureus were analyzed computationally for the presence of HLA class II binding peptides. Fifteen-mer peptides were generated, and their binding to 26 HLA class II alleles was predicted. The structural feasibility of the peptides binding to HLA-II was studied using molecular docking. Of the 16,724 peptides generated, 6991 (41.8%) were predicted to bind to any one of the alleles with an IC50 value below 50 nM. Comparative sequence analysis revealed that only 545 of the strong binding peptides are non-self in the human system. Approximately 50% of the binding peptides were monoallele-specific. Moreover, approximately 95% of the predicted strong binding non-self peptides interacted with the binding groove of at least one HLA class II molecule with a glide score better than !10 kcal/mol. On the basis of the analysis of the strength of binding, non-self presentation in the human host, propensity to bind to a higher number of alleles, and energetically favorable interactions with HLA molecules, a set of 11 CD4+ T cell epitopes that can be used as vaccine candidates was identified.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 1; 43-54
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytopathology of canine lymphomas (100 cases)
    Autorzy:
    Sapierzynski, R.
    Micun, J.
    Jagielski, D.
    Jurka, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/31296.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cytopathology
    canine lymphoma
    lymphoma
    dog
    malignant lymphoma
    tumour
    animal disease
    occurrence
    T cell
    biopsy
    Opis:
    Malignant lymphoma is one of the most common malignant tumours occurring in dogs. Fine needle aspiration biopsy (FNAB) is an excellent, specific diagnostic procedure used to assess pathological processes in lymph nodes. The aim of the present study was to conduct a cytopathological analysis of lymphoma in dogs and to analyse some epidemic aspects of occurrence of lymphoma in 100 dogs using Giemsa stained slides. Samples were obtained by fine needle aspiration biopsy, fine needle non-aspiration biopsy, lymph node impression smears and by examination of body cavity effusions. The determination of the type and subtype of tumour was made on the basis of the updated Kiel classification adopted for dogs. Based on cytopathological analysis, the lymphoma was diagnosed in 100 dogs: 44 were female and 56 male. The animals’ age ranged from 1.5 year to 15 years (median: 7.5 years), the animals were of different breeds (72% of all dogs belonged to 28 different breeds) and crossbreeds (28%). In 29% of dogs the regional or general lymphadenomegaly was the only clinical sign observed, in remaining cases (71%) at least one abnormality connected to lymphoma was found. Among all diagnosed lymphomas, high-grade lymphomas were more prevalent (86% of all cases) than low-grade lymphomas (14% of all cases). The possibility of boxers having a predisposition to T cell lymphoma development could be also suspected.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The presence of CD25 on bovine WC1 plus gamma delta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma
    Autorzy:
    Maslanka, T.
    Jaroszewski, J.J.
    Markiewicz, W.
    Ziolkowski, H.
    Barski, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/32183.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cattle
    WC1 cell
    T cell
    T lymphocyte
    transforming growth factor-beta
    interleukin 10
    interferon-gamma
    CD25 antigen
    CD25 molecule
    Opis:
    WC1+ cells in cattle exhibit both regulatory and effector activities. However, it has not been elucidated whether they are so plastic that both activities co-exist in one cell or there are separate subpopulations of effector and regulatory cells. Since the production of IFN-γ and IL-10 seems to be related to WC1+ cells’ effector and regulatory function, respectively, the main aim of this study was to determine whether those cytokines are produced by separate subpopulations of WC1+, or are co-produced by the same cells. Due to increasingly frequent emphasised role of consumption of IL-2 in the mechanism of suppressor action of mouse CD25+CD4+ T regulatory cells, expression of the receptor’s α chain for interleukin 2 (CD25) on WC1+ lymphocytes has been evaluated. An average of 5.21% of WC1+ cells obtained from PBMCs of 12-month-old heifers show constitutive expression of the CD25 molecule, with CD25highWC1+ and CD25lowWC1+ cells accounting for 1.05% and 4.10% of WC1+ lymphocytes, respectively. For detection of intracellular cytokine production, PBMCs were stimulated with concanavalin A. Both IFN-γ- and IL-10-producing cells within the CD25-WC1+ and CD25+WC1+ subpopulations were mainly separate subpopulations. The average percentage of IFN-γ+IL-10-, IFN-γ-IL-10+ and IFN-γ+IL-10+ cells among CD25-WC1+ lymphocytes was 4.03%, 2.67% and 0.51%, respectively. A positive correlation was observed between the presence of the CD25 molecule on WC1+ lymphocytes and production of IL-10 and TGF-β, because the average percentage of IFN-γ-IL-10+ and IFN-γ+IL-10+ among CD25+WC1+ lymphocytes was 3 and 4.5 times higher as compared to the corresponding cells in the CD25-WC1+ subpopulation, whereas the percentage of IFN-γ+IL-10- cells in both the subpopulations was not significantly different. The percentage of TGF-β+ cells within the CD25+WC1+ subpopulation was 2.72 times as high as that of CD25-WC1+ lymphocytes. Therefore, with respect to the production of IFN-γ, IL-10 and TGF-β, CD25+WC1+ lymphocytes turn out to have a more suppressor profile than CD25-WC1+.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Limited pattern of TCR delta chain gene rearrangement on the RNA level in multiple sclerosis
    Autorzy:
    Nowak, J
    Januszkiewicz, D.
    Pernak, M.
    Hertmanowska, H.
    Nowicka-Kujawska, K.
    Rembowska, J.
    Lewandowski, K.
    Nowak, T.
    Wender, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2041829.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    patient
    human genetics
    brain
    multiple sclerosis
    autoagression
    T cell receptor rearrangement
    gene rearrangement
    RNA level
    chain gene
    Źródło:
    Journal of Applied Genetics; 2001, 42, 4; 531-540
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Low Leakage and Robust Sub-threshold SRAM Cell Using Memristor
    Autorzy:
    Mustaqueem, Zeba
    Ansari, Abdul Quaiyum
    Akram, Md Waseem
    Powiązania:
    https://bibliotekanauki.pl/articles/2200681.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    6T SRAM cell
    memristor
    power dissipation
    read and write operation
    leakage current
    stability
    non-volatile circuit
    Opis:
    This work aims to improve the total power dissipation, leakage currents and stability without disturbing the logic state of SRAM cell with concept called sub-threshold operation. Though, sub-threshold SRAM proves to be advantageous but fails with basic 6T SRAM cell during readability and writability. In this paper we have investigated a non-volatile 6T2M (6 Transistors & 2 Memristors) sub-threshold SRAM cell working at lower supply voltage of VDD=0.3V, where Memristor is used to store the information even at power failures and restores previous data with successful read and write operation overcomes the challenge faced. This paper also proposes a new configuration of non-volatile 6T2M (6 Transistors & 2 Memristors) subthreshold SRAM cell resulting in improved behaviour in terms of power, stability and leakage current where read and write power has improved by 40% and 90% respectively when compared to 6T2M (conventional) SRAM cell. The proposed 6T2M SRAM cell offers good stability of RSNM=65mV and WSNM=93mV which is much improved at low voltage when compared to conventional basic 6T SRAM cell, and improved leakage current of 4.92nA is achieved as compared.
    Źródło:
    International Journal of Electronics and Telecommunications; 2022, 68, 4; 667--676
    2300-1933
    Pojawia się w:
    International Journal of Electronics and Telecommunications
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. I. Preparation of the bacterial cells, transformation and isolation of the bluescript plasmid
    Autorzy:
    Borowicz, B P
    Powiązania:
    https://bibliotekanauki.pl/articles/66849.pdf
    Data publikacji:
    1999
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    transformation
    promoter system
    gene expression
    cloning
    I-18 C gene
    isolation
    T7 RNA polymerase
    plasmid
    Chironomus tentans
    bacterial cell
    polymerase chain reaction
    DNA fragment
    Opis:
    The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the competent E. coli cells of the XL-1 strain needed for the transformation with the ligation reaction products, is presented. Also the transformation of these bacterial cells with the bluescript plasmid and its isolation for these cloning experiments, are described.
    Opisano technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania kompetentnych komórek E. coli szczepu XL-1, poddawanych następnie transformacji produktami reakcji ligacji. Przedstawiono także zastosowaną technologię transformacji wymienionych komórek bakteryjnych przy użyciu plazmidu - blueskrypt oraz izolację tego plazmidu, w celu jego zastosowania w wymienionych eksperymentach klonowania.
    Źródło:
    Journal of Plant Protection Research; 1999, 39, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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