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Wyświetlanie 1-15 z 79
Tytuł:
Enzyme activities regulating ROS metabolism in olive ( Olea europaea L.) seeds
Autorzy:
Zienkiewicz, A.
Jimenez-Quesada, M.
Traverso, J.
Zafra, A.
Lopez-Huertas, E.
Zienkiewicz, K.
Castro, A.
Rodriguez-Garcia, M.
Alche, J.
Powiązania:
https://bibliotekanauki.pl/articles/79927.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
enzyme activity
reactive oxygen species
metabolism
olive
Olea europaea
seed
antioxidative enzyme
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of three pesticides on superoxide dismutase and glutathione-S-transferase activities and reproduction of Daphnia magna
Autorzy:
Song, Y.
Chen, M.
Zhou, J.
Powiązania:
https://bibliotekanauki.pl/articles/204826.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
enzyme activity
reproductive parameter
pesticide
Daphnia magna
Opis:
Applying pesticides to crops is one of the causes of water pollution by surface runoff, and chlorpyrifos, trifluralin and chlorothalonil are used respectively as insecticide, herbicide and fungicide for crop plants widely. To explore effects of three pesticides on aquatic organisms, superoxide dismutase (SOD) and glutathione S-transferase (GST) activities were determined after 24 h and 48 h exposure of D. magna with ages of 6–24 h to several low concentrations of chlorpyrifos (0.36, 0.72, 1.43, 2.86, 5.72 μg∙L−1), trifluralin (0.17, 0.33, 0.66, 1.33, 2.65 mg∙L−1) and chlorothalonil (0.09, 0.18, 0.36, 0.72, 1.43 mg∙L−1) respectively. Main reproductive parameters including first pregnancy time, first brood time, the number of first brood and total fecundity after 21 d exposures at the same concentrations of pesticides as described above were also measured. The results showed that the activities of GST increased in lower concentrations and decreased in higher concentrations after 24 h exposure to three pesticides, respectively. The activities of SOD showed the same changes after 48 h exposure. With the time prolonged, the activities of GST decreased while the activities of SOD increased. After 21 d exposure, the first pregnancy time and first brood time were delayed, while the number of the first brood and total fecundity per female decreased with increasing concentrations. These results corroborated that GST activity was more sensitive to those pesticides than SOD activity, and there was a significant relationship between total fecundity and pesticides-dose(r>0.94, n=6), GST activity after 48 h exposure and total fecundity after 21 d exposure (r>0.92, n=6).
Źródło:
Archives of Environmental Protection; 2017, 43, 1; 80-86
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular forms of selected antioxidant enzymes in dog semen – electrophoretical identification
Autorzy:
Koziorowska-Gilun, M.
Strzezek, R.
Powiązania:
https://bibliotekanauki.pl/articles/32130.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular form
antioxidative enzyme
dog
semen
electrophoresis
identification
spermatozoon
enzyme
superoxide dismutase
catalase
glutathione peroxidase
reactive oxygen species
Opis:
The aim of the study was the electrophoretical identification of molecular forms of selected antioxidant enzymes in dog semen. Ejaculates to be studied were chosen from five dogs, aged from two to eight years. Polyacrylamide gel electrophoresis was carried out under non-denaturing conditions and then gels were stained for the activity of the following enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Sperm homogenates and all fractions (pre-spermatic, spermatic and post-spermatic) of dog ejaculate demonstrated one protein band with SOD activity characterized by low electrophoretic mobility. Based on the confirmed sensitivity to H₂O₂, it can be assumed that the detected SOD is an enzyme containing ions of Zn²⁺ and Cu²⁺ (Cu,Zn SOD). In sperm homogenates one protein band with GPx activity was characterized by high electrophoretic mobility, whereas in the spermatic and post-spermatic fractions of dog ejaculate three protein bands with different (low, medium and high) electrophoretic mobility were identified. CAT molecular forms were not found in either sperm homogenates or in the analyzed fractions of ejaculate.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tripeptidyl peptidase I activity in porcine lumbar spinal ganglia – a histochemical study
Autorzy:
Vodenicharov, A.P.
Dimitrova, M.
Tsandev, N.S.
Stefanov, I.S.
Powiązania:
https://bibliotekanauki.pl/articles/2087120.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
enzyme histochemistry
pig
spinal ganglia
tripeptidyl peptidase I
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 3; 409-414
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical composition and cellulase-assisted extraction of total saponins from Gomphrena celosioides Mart.
Autorzy:
Yen, N.T.H.
Quoc, L.P.T.
Powiązania:
https://bibliotekanauki.pl/articles/2096279.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioactive compounds
composition
enzyme
extraction
Gomphrena celosioides Mart.
Opis:
The major purpose of the present study was to determine the chemical composition of dried Gomphrena celosioides Mart., including crude protein, lipid, total sugar, reducing sugar, ash, crude fiber, moisture, crude pectin, and total saponins in particular. This plant contains a large percentage of total saponins, which are widely used in food technology and pharmaceutical industries. In our study, the conditions for the extraction of total saponins from the plant were optimized, with special focus on cellulase concentration, pH, hydrolyzation time, and temperature. The highest amount of total saponins content (TSC) was 1.550±0.016% under optimal extraction conditions (cellulase concentration: 0.7% (v/w), hydrolyzation time: 4 h, hydrolyzation temperature: 60EC, and pH value: 5). Accordingly, the total saponins from G. celosioides Mart. were easily extracted.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 361-367
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed spermin Holstein-Friesian bulls
Autorzy:
Kaminski, S.
Hering, D.M.
Kordan, W.
Lecewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087549.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bull
sperm
polymorphism
CFTR gene
antioxidant enzyme activity
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 221-225
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phytoremediation of phenol using Polygonum orientale and its antioxidative response
Autorzy:
Wang, K.
Cai, J.
Xie, S.
Feng, J.
Wang, T.
Powiązania:
https://bibliotekanauki.pl/articles/205372.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Polygonum orientale
phenol
phytoremediation
antioxidant enzyme
osmotic regulator
Opis:
Polygonum orientale with beautiful red flowers can be found as one dominant species in the vicinity of most water bodies and wetlands in China. However, its phytoremediation potential has not been sufficiently explored because little is known about its resistance to inorganic or organic pollutants. We investigated P. orientale response to low and moderate levels of phenol stress (≤ 80 mg L-1). Endpoints included phenol tolerance of P. orientale and the removal of the pollutant, antioxidant enzyme activities, damage to the cell membrane, osmotic regulators and photosynthetic pigments. In plant leaves, phenol stress significantly increased the activities of peroxidase (POD) and catalase (CAT), as well as the contents of proline, soluble sugars and carotenoids, whereas superoxide dismutase (SOD), H2O2 and electrolyte leakage (EL) levels remained unaltered. On the other hand, there were significant decreases of soluble protein and chlorophyll contents. We demonstrated that, in combination with phenol tolerance and its removal, P. orientale has efficient protection mechanisms against phenol-induced oxidative damage (≤ 80 mg L-1). We propose that P. orientale could be used as an alternative and interesting material in the phytoremediation of phenol.
Źródło:
Archives of Environmental Protection; 2015, 41, 3; 39-46
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens
Autorzy:
Farahani, A.S.
Taghavi, M.
Powiązania:
https://bibliotekanauki.pl/articles/65764.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidative enzyme
enzyme
mung bean
Vigna radiata
plant response
host pathogen
non-host pathogen
resistance
bacterial pathogen
ascorbate peroxidase
catalase
peroxidase
superoxide dismutase
Opis:
The natural resistance against the majority of potential pathogens that exist in most plant species is known as non-host resistance. Several reports suggest the role of antioxidant enzymes in non-host resistance. We assayed the expression or activity of four scavenging enzymes during non-host pathogen-plant interaction (Xanthomonas hortorum pv. pelargonii/mung bean) and host pathogen-plant interaction (Xanthomonas axonopodis pv. phaseoli/mung bean). The expression of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the enzyme activity of catalase (CAT) and peroxidase (POX) were investigated. The activities of CAT and POX were higher during non-host pathogen invasion vs. host pathogen attack. The expression of SOD and APX were also different between compatible and incompatible interactions. The expression of SOD and APX were higher in the incompatible compared to the compatible interaction. Additionally, induction of the antioxidant enzymes in response to non-host pathogen was earlier than induction in response to host pathogen. Such information is important for plant breeders, and useful when looking for alternative control strategies as well.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Light-triggered protochlorophyllide to chlorophyllide reduction – new insights into enzyme-substrate interactions
Autorzy:
Gabruk, M.
Kruk, J.
Mysliwa-Kurdziel, B.
Powiązania:
https://bibliotekanauki.pl/articles/79867.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biosynthesis
chlorophyll
photosynthetic pigment
protochlorophyllide
chlorophyllide
enzyme substrate interaction
fluorescence
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gold Nanoparticles Like A Matrix For Covalent Immobilization Of Cholesterol Oxidase – Application For Biosensing
Nanocząstki złota jako matryca do immobilizacji oksydazy cholesterolu – zastosowanie w biosensorach
Autorzy:
Wojnarowska, R.
Polit, J.
Broda, D.
Gonchar, M.
Sheregii, E. M.
Powiązania:
https://bibliotekanauki.pl/articles/352497.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
gold nanoparticles
biofunctionalization
enzyme
FTIR
SERS
nanocząstki złota
biofunkcjonalizacja
enzymy
Opis:
Gold nanoparticles are emerging as promising agents for various areas of material science as well as nanotechnology, electronics and medicine. The interest in this material is provided due to its unique optical, electronic and molecular-recognition properties. This paper presents results of preparation, characterization and biofunctionalization of gold nanoparticles. Nanoparticles have been conjugated with the cholesterol oxidase enzyme in order to prepare the active element for biosensors. Cholesterol oxidase is one of the most important analytical enzyme, used for cholesterol assay in clinical diagnostics, and there is still a necessity in improvement of existing analytical techniques, including bio-nanotechnological approaches based on modern nanosystems. The prepared bio-nanosystem was characterized by the enzyme activity test. Obtained results showed a stable binding of the enzyme with nanoparticles and preserved the bioactivity approves which gives possibility to use the prepared bio-nanosystems for analytical purposes. The Surface Plazmon Resonance and the Surface Enhanced Raman Scattering as specific for nanocurriers effects were observed what enable us to research the oscillation spectra of enzyme. These spectra were compared with ones obtained by IR-spectroscopy. The vibrational lines are attributed to chemical functional groups existing in enzyme, for example, amino acids, amide groups as well as flavin – adenine dinucleotide cholesterol oxidase prosthetic group. By this way the identified spectral lines can be used as a distinguishing mark of enzyme.
Nanocząstki złota zdają się być niezwykle obiecującym materiałem, który z powodzeniem może być wykorzystywany w różnych dziedzin inżynierii materiałowej, nanotechnologii, elektroniki czy medycyny. Zainteresowanie tym materiałem wynika z posiadanych przez niego unikatowych właściwości optycznych, elektronicznych i identyfikacji na poziomie molekularnym. W artykule przedstawiono wyniki prowadzonych badań dotyczących przygotowania, charakterystyki i biofunkcjonalizacji nanocząstek złota. Celem wytwarzania i łączenia nanocząstek złota z enzymem oksydazą cholesterolową było opracowanie aktywnego biologicznie elementu biosensora. Oksydaza cholesterolowa jest jednym z najważniejszych enzymów analitycznych, stosowanych w diagnostyce klinicznej do oznaczenia stężenia cholesterolu. Istnieje potrzeba doskonalenia istniejących metod analitycznych, między innymi poprzez wykorzystanie nowoczesnych nanomateriałów. Przeprowadzono badania aktywności enzymu immobilizowanego, jak również dokonano charakterystyki danego bionanosystemu za pomocą wybranych metod optycznych. Na podstawie uzyskanych wyników można stwierdzić, że wiązanie enzymu z nanocząstkami jest stabilne, a aktywność biologiczna została zachowana, co pozwala na przygotowanie bio-nanosystemu do celów analitycznych. Zaobserwowano również wystąpienie dwóch efektów optycznych charakterystycznych w przypadku zastosowania odpowiednich nanonośników: Powierzchniowego Rezonansu Plazmonów oraz Powierzchniowo Wzmocnionego Rozproszenia Ramanowskiego. Uzyskane widma były porównywane z widmami w podczerwieni. Zaobserwowane linie przypisano do charakterystycznych grup funkcyjnych, aminokwasów, wiązań peptydowych, jak również dinukleotydu flawinoadeninowego czyli grupy prostetycznej oksydazy cholesterolowej. Zidentyfikowane linie mogą być wykorzystywane jako marker analityczny dla danego enzymu.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 3; 2289-2296
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a recombinant NP protein based indirect ELISA for the detection of antibodies against Newcastle disease virus and differentiation of infected or recombinant vaccine immunized chickens
Autorzy:
Domańska-Blicharz, K.
Tyborowska, J.
Sajewicz-Krukowska, J.
Olszewska-Tomczyk, M.
Rąbalski, Ł.
Kucharczyk, K.
Szewczyk, B.
Śmietanka, K.
Powiązania:
https://bibliotekanauki.pl/articles/2087467.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Newcastle disease virus
serology
Enzyme-Linked Immunosorbent assay
nucleoprotein
differentiation
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 531-540
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Defense responses of rice plant to Monographella albescens attack
Autorzy:
Almas, D.E.
Kamrodi, A.R.
Powiązania:
https://bibliotekanauki.pl/articles/2084805.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
defense-signaling genes
enzyme activities
Monographella albescens
rice
RT-qPCR
Opis:
Leaf scald, caused by the necrotrophic fungus Monographella albescens, is one of the main threats to rice (Oryza sativa L.) around the world. This disease decreases yields in rice by up to 30% because of dead leaf tissue, damaged seeds, and sterile flowers. Currently, there is limited knowledge about the molecular mechanisms involved in rice plant resistance against this pathogen. For this purpose, six commercial cultivars of rice were primarily screened for M. albescens infection and development. Dasht and Salari were found to be the most resistant and susceptible to M. albescens infection, respectively. The plants were kept in a greenhouse at 29 ± 2°C during the day and 26 ± 2°C at night with a relative air humidity of 85 ± 5%. Forty-five days after sowing, the plants with three biological replications were inoculated by transferring a PDA disc (0.3 cm2) containing M. albescens mycelia to the middle third of the 7th, 8th, and 9th completely open leaves. The leaves were collected 24, 48, 72, 96 and 120 hai. Leaf samples were also collected from the non-inoculated plants (0 h) to serve as controls. Real-time quantitative PCR (RT-qPCR) showed rapid induction and significant accumulation of jasmonic acid (JA) and ethylene (ET) responsive genes such as lipoxygenase (LOX), allene oxide synthase 2 (Aos2), jasmonic acid carboxyl methyltransferase 1 (JMT1) and ACC synthase 1 (ACS1) in the resistant Dasht cultivar after infection with M. albescens. Furthermore, the transcripts of salicylic acid (SA) responsive phenyl alanine ammonia lyase 1 (PAL1) and nonexpressor of pathogenesis-related genes 1 (NPR1) genes were induced in the incompatible interaction. The activities of the defense enzymes superoxide dismutase (SOD), peroxidase (POX) and glutathione reductase (GR) increased strongly in Dasht in response to M. albescens infection. In addition, there was an increase in the H2 O2 levels in the leaves of the Dasht cultivar during the infectious period of M. albescens associated with the enhancement of catalase (CAT) activity as well as higher levels of malondialdehyde (MDA). This is the first study on the interaction between rice and M. albescens at the molecular level. It can contribute to understanding how rice responds to pathogen infection, as well as assist with future research plans of molecular breeding regarding the tolerance to leaf scald disease.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 535-543
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Redox state modulation in rice and its implications for plant development and stress responses
Autorzy:
Caverzan, A.
Ribeiro, C.
Lazzarotto, F.
Passaia, G.
Rosa, S.
Garighan, J.
Piovesana, M.
Carvalho, F.
Albenisio, J.
Margis, R.
Margis-Pinheiro, M.
Powiązania:
https://bibliotekanauki.pl/articles/79908.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
scavenging enzyme
detoxification
redox state
rice
plant development
stress response
plant cell
biochemical property
antioxidative enzyme
ascorbate peroxidase
glutathione peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The activity of DGAT and PDAT enzymes in the developing seeds of Brassica napus
Autorzy:
Demski, K.
Furmanek, T.
Jasieniecka, K.
Banas, A.
Powiązania:
https://bibliotekanauki.pl/articles/80079.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
diacylglycerol acyltransferase
PDAT enzyme
Brassica napus
developing seed
triacylglycerol
phosphatidylcholine
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A new method for detecting cross-inhibition effects in the environmental biocatalytic processes
Autorzy:
Herke, Z.
Maskow, T.
Nemeth, Z.I.
Powiązania:
https://bibliotekanauki.pl/articles/80851.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biocatalysis
microorganism
enzyme
bioremediation
inhibition
modelling
regression analysis
principal component analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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