Temat: DNA hybridization - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: DNA fingerprints generated by R.18.1 DNA probe in two stocks of chickens: Green Legged Patridgenous [GLP] and Rhode Island Red [RIR]
Autorzy:: Rosochacki, S J
Hillel, J
Jaszczak, K
Zawadzka, M
Powiązania:: https://bibliotekanauki.pl/articles/2046824.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: multi-locus DNA probe
hybridization
animal breeding
poultry
molecular marker
DNA
chicken
Opis:: Hybridization of a multi-locus DNA probe, R 18.1, to genomic DNA from poultry showed a highly polymorphic fingerprint pattern. The detected DNA fingerprints are individually specific and differ between Green Legged Patridgenous (GLP) and Rhode Island Red (RIR) stock of chickens. The average numbers of detected bands in RIR were 18.68 and in GLP - 15.33, but the average band sharing levels were 0.619 and 0.431, respectively. The level of polymorphism may be connected possibly with a higher level of inbreeding in the examined stock of chicken.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 173-178
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Segmented hybridization probes: modulating target affinity and base pairing selectivity
Autorzy:: Egetenmeyer, S.
Geiger, E.
Richert, C.
Powiązania:: https://bibliotekanauki.pl/articles/81071.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: oligonucleotide
DNA
RNA
hybridization
base pairing
molecular biology
polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: DNA polymorphism in locus D1S80 in Poland. DNA profiling and detection of new alleles by heteroduplex formation between alleles of the same size
Autorzy:: Kwiatkowska, J
Dziechciowska, K
Lisiecka, D
Slomski, R
Powiązania:: https://bibliotekanauki.pl/articles/2046677.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: DNA sequence
polymorphism
Polska
hybridization
Polish population
heteroduplex formation
DNA polymorphism
same size
polymerase chain reaction
allele
distribution
Opis:: We have analysed allele distribution at the highly polymorphic variable number of tandem repeats (VNTR) locus D1S80 (pMCT118) in the Polish population using the, polymerase chain reaction (PCR) technique. Characteristics of the D1S80 locus makes it a very useful marker for population genetic research, genetic linkage studies and forensic identification of individuals. During our routine application of the D1S80 marker to paternity testing in several cases of homozygosity detected by polyacrylamide gel electrophoresis, heteroduplex formation for alleles 18 and 24 was also observed. Direct sequencing of PCR products revealed that alleles 18 and 24 of locus D1S80 actually represent a mixture composed of different sequences. Our observations indicate that identification of some 18 and 24 VNTR alleles based only on size estimated in electrophoretic analyses could lead to errors in paternity testing and DNA profiling.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 335-341
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1
Autorzy:: Pusch, C
Wang, Z.
Roe, B.
Blin, N.
Powiązania:: https://bibliotekanauki.pl/articles/2048293.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
human chromosome
chromosome
hybridization
genetic change
linear map
polymerase chain reaction
DNA
cosmid
cDNA
transcriptional orientation
Opis:: The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.
Źródło:: Journal of Applied Genetics; 1998, 39, 2; 199-204
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Transcriptome sequencing: next generation approach to RNA functional analysis
Autorzy:: Zmienko, A.
Jackowiak, P.
Figlerowicz, M.
Powiązania:: https://bibliotekanauki.pl/articles/81137.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA molecule
functional analysis
RNA sequence
transcriptome
isoform
DNA microarray
qualitative change
quantitative change
gene expression
hybridization
oligonucleotide probe
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution
Autorzy:: Idziak, D.
Hasterok, R.
Betekhtin, A.
Borowska-Zuchowska, N.
Braszewska-Zalewska, A.
Chwialkowska, K.
Gorkiewicz, R.
Kus, A.
Kwasniewska, J.
Kwasniewski, M.
Robaszkiewicz, E.
Siwinska, D.
Wolny, E.
Chrominski, K.
Tkacz, M.
Powiązania:: https://bibliotekanauki.pl/articles/951271.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Brachypodium distachyon
model plant
grass
genome structure
Expressed Sequence Tag programme
fluorescent in situ hybridization
chromosome painting
DNA methylation
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Cryptic rearrangements of chromosome 12 in testicular germ cell tumors with or without the specific i[12p] marker
Autorzy:: Grygalewicz, B
Pienkowska-Grela, B.
Woroniecka, R.
Powiązania:: https://bibliotekanauki.pl/articles/2043149.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: aberration
in situ
isochromosome
cytogenetic analysis
microdissection
testicular germ cell tumour
karyotype
cell culture
nonseminoma
fluorescence
hybridization
seminoma
chromosome 12
DNA
Źródło:: Journal of Applied Genetics; 2000, 41, 2; 123-131
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Structure and functions of lampbrush chromosomes
Autorzy:: Andraszek, K.
Smalec, E.
Powiązania:: https://bibliotekanauki.pl/articles/81204.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: lampbrush chromosome
oocyte
transcriptional activity
physiological process
morphological structure
cytogenetic technique
in situ hybridization
DNA replication
cytogenetic investigation
karyotype evolution
genome mapping
meiotic chromosome
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Search for mouse gene related to GA733 human tumor antigen gene
Autorzy:: Zielewicz, J
Skrzypczak, M.
Wojcierowski, J.
Powiązania:: https://bibliotekanauki.pl/articles/2048195.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: human antigen
glycoprotein
human genetics
mice
gene sequence
adenocarcinoma
cancer therapy
electrophoresis
carcinoma cell
bacteriophage
antigen
gene
mouse
oncoantigen
hybridization
tumour etiology
tumour
DNA
Opis:: Human antigen GA733-1, defined as 40 kDa cell glycoprotein, is one of the antigens associated with gastrointestinal carcinomas. Its studies may contribute to the tumor etiology and therapy effects in animal model.
Źródło:: Journal of Applied Genetics; 1995, 36, 3; 273-277
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Monitoring of cellular chimerism in patients after sex-mismatched bone marrow transplantation: technical report
Autorzy:: Jolkowska, J
Ladon, D.
Wachowiak, J.
Witt, M.
Powiązania:: https://bibliotekanauki.pl/articles/2043435.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: in situ
allogeneic transplantation
chimerism
molecular analysis
transplantation
minimal residual disease
acute myeloblastic leukemia
polymorphic microsatellite
bone marrow
hybridization
polymerase chain reaction
DNA
detection
Źródło:: Journal of Applied Genetics; 2000, 41, 3; 209-212
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber [Cucumis sativus L.]
Autorzy:: Plader, W
Hoshi, Y.
Malepszy, S.
Powiązania:: https://bibliotekanauki.pl/articles/2044260.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: heterochromatin
4',6-diamidino-2-phenylindole
euchromatin segment
hybridization
chromosome identification
mitotic chromosome
prometaphase chromosome
somatic cell
cucumber
DNA
chromomycin A3
Cucumis sativus
Opis:: Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A₃ (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA⁺) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA⁺DAPI⁻) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA⁺DAPI⁻ band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA⁺ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA⁺ band at the short arm and a clear CMA⁺DAPI⁻ band at the pericentromeric region. Chromosome 6 had a CMA⁺ band at the distal region and a weak CMA⁺ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA⁺ band in the long arm and a CMA⁺ DAPI⁻ band in the pericentromeric region.
Źródło:: Journal of Applied Genetics; 1998, 39, 3; 249-258
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
Autorzy:: Humphreys, M W
Thomas, H M
King, I P
Morgan, W G
Meredith, M R
Harper, J A
Humphreys, M O
Bettany, A J E
Dalton, S J
James, A R
Ougham, H J
Thomas, H
Powiązania:: https://bibliotekanauki.pl/articles/2046675.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
in situ
tissue culture
gene transfer
chromosome segment
anther culture
androgenesis
plant breeding
introgression mapping
fluorescence
hybridization
Lolium-Festuca complex
drought resistance
gene isolation
tetraploid hybrid
DNA
meiosis
grass
chromosome behaviour
Opis:: Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 273-284
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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