Temat: Amylase - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Polycarbonate biodegradation by newly isolated Bacillus strains
Autorzy:: Arefian, Mojgan
Tahmourespour, Arezoo
Zia, Mohammadali
Powiązania:: https://bibliotekanauki.pl/articles/205230.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biodegradation
Bacillus cereus
amylase
lipase
Polycarbonate
Bacillus megaterium
Opis:: As polycarbonate is frequently used in many products, its accumulation in landfills is absolutely harmful to the environment. The aims of this study were the screening and isolation of polycarbonate-degrading bacteria (PDB) and the assessment of their ability in the degradation of polycarbonate (PC) polymers. Nine-month buried- -PC films were used for PDB isolation and identification. The biodegradation ability of the isolates was determined by growth curve, clear zone formation, lipase and amylase production, AFM and FTIR. Bacillus cereus and Bacillus megaterium were identified and considered as PDB. The degradation ability of B. megaterium was significantly higher than that of B. cereus. Both were lipase and amylase positive. AFM and FTIR results showed the initiation of bacterial attachment. The PC biodegradation ability of isolates can be very efficient. Finding such efficient isolates (which was less studied before) will promise a decrease in plastic contamination in the future.
Źródło:: Archives of Environmental Protection; 2020, 46, 1; 14-20
2083-4772
2083-4810
Pojawia się w:: Archives of Environmental Protection
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Starch-iodine assay method underestimates alpha-amylase inhibitory potential of antioxidative compounds and extracts
Autorzy:: Ononamadu, C.J.
Ezeigwe, O.C.
Owolarafe, T.A.
Ihegboro, G.O.
Lawal, T.A.
Salawu, K.
Umeoguaju, U.F.
Aminu, I.
Powiązania:: https://bibliotekanauki.pl/articles/2096980.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: α-amylase inhibition
starch-iodine
diabetes mellitus
limitations
decolorization
Opis:: Starch-iodine assay method for the determination of α-amylase activity is also used in screening extracts for α-amylase inhibitors. However, there are indications that this method may not be appropriate for screening some classes of compounds or plant extracts. The present study investigated the limitation(s) of this method in screening plant extracts/compounds for α-amylase inhibition. A crude methanol extract (CR) of Dacryodes edulis, its solvent fractions (ethyl acetate (EA), aqueous methanol (AM), and hexane (HX)), quercetin (QC), and benzoic acid (BA) were used for this study. The phytochemical content and antioxidant activity were screened spectrophotometrically. α-Amylase inhibition (expressed in percentage and as IC50) was determined by starch-iodine method approach I and II (ST-ID I and ST-ID II, respectively) and dinitrosalicylic acid (DNSA) as the control method. The results showed that the extracts/compounds (AM, EA, and QC) with significantly high polyphenolic content, antioxidant activity, and starch-iodine complex decolorization effect yielded contrary results of α-amylase inhibition when the results of ST-ID I and II methods were compared to that of the DNSA method. The other test samples (CR, HX, and BA) yielded similar results for all the three methods. The result also showed the decolorization (%) of starch-iodine complex by the test samples correlated significantly (r = 0.877, P < 0.05) with DPPH reduction (%). In conclusion, the present study showed that the starch-iodine method is not appropriate for screening antioxidative extracts/compounds for α-amylase inhibitors – they decolorize the assay reagent in a manner similar to DPPH reduction and hence confound the result.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 45-54
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Germination, alpha-, beta-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3
Autorzy:: Bialecka, B
Kepczynski, J.
Powiązania:: https://bibliotekanauki.pl/articles/19888.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Amaranthus caudatus
seed
germination
alpha-amylase activity
beta-amylase activity
dehydrogenase activity
water stress
ethylene
gibberellin A3
polyethylene glycol
ethephon
water deficit
laboratory condition
plant
environmental stress
Opis:: Amaranthus caudatus L. seed germination was studied under different levels of water deficit induced by PEG 6000 in laboratory conditions. PEG at osmotic potentials -0.2 to -0.3 MPa at 24°C in darkness delayed germination and reduced final germination percentage. PEG solutions at osmotic potential lower than -0.3 MPa almost totally blocked seed germination. Ethephon was much more effective than GA3 in reversing PEG-caused inhibition of A. caudatus seed germination. PEG decreased α-amylase activity after 14 h incubation. It decreased β-amylase activity after 14 and 20 h, and caused an increase in total dehydrogenase activity only after 20 h of incubation. Unlike GA3, ethephon increased α-amylase activity in seeds after 12 and 14 h of incubation under water deficit. After 20 h of incubation there was no difference in α-amylase activity in any of the treatments. Neither ethephon nor GA3 affected the activity of β-amylase and dehydrogenase.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2010, 52, 1; 7-12
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Involvement of ROS and its scavenging enzymes in the response of dormant Avena fatua L. caryopses to karrikinolide (KAR1)
Autorzy:: Cembrowska-Lech, D.
Kepczynski, J.
Powiązania:: https://bibliotekanauki.pl/articles/80050.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
reactive oxygen species
scavenging enzyme
Avena fatua
caryopsis
germination
dehydrogenase activity
amylase
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Interaction between larval alpha-amylase of the tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) and proteinaceous extracts from plant seeds
Autorzy:: Esmaeily, M.
Bandani, A.R.
Powiązania:: https://bibliotekanauki.pl/articles/65574.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: interaction
larva
alpha-amylase
tomato leafminer
Tuta absoluta
Lepidoptera
Gelechiidae
plant extract
seed
plant seed
Opis:: The tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), is one of the most destructive pest of solanaceae and it prefers tomato (Solanum lycopersicum L.). The aim of the current study was to investigate the effects of a wide range of seed proteinaceous extracts from different plant families against T. absoluta α-amylase activity. The effect of pH on the inhibitory activity of seed extracts showed that seed extracts of amaranth along with a wheat cultivar (Alvand, Aflak, Sarvdasht, Alborz, and Kavir) produced more than a 50% inhibition of the insect amylase. Aflak wheat seed extract at 10 μg, inhibited 81% of the insect amylase. This percent was the highest inhibition achieved. The other proteinaceous seed extracts had a lower effect on the enzymatic activity. Probit analysis showed that Aflak, Kavir, Alborz, Alvand, Sarvdasht, and amaranth inhibited the amylase activity with an I50 of 1.94, 3.24, 3.46, 3.31, 4.97, and 15.39 μg, respectively. The effect of pH on the inhibition of the α-amylase showed the highest inhibition of Amaranth and wheat, at a pH value of 8.0, which corresponds to the pH of the insect’s gut. Gel electrophoresis assays confirmed the spectrophotometric assays showing that the α-amylase of the insect gut was affected by the presence of the seed extracts. In the gel assay, a high concentration (14 μg protein) of amaranth proteinaceous seed extract greatly decreased the intensity of the α-amylase band. A high concentration of the Aflak wheat cultivar (10 μg protein) caused the disappearance of the amylase band in the gel. Thus, it is concluded that the physiochemical environment of the insect gut affects the interaction between digestive α-amylase and the metabolites. The experiments showed that seed proteinaceous extracts from non-host plant species, produced more inhibition of the insect amylase when compared to the host plant species. It appears that with evolution, adaptation took place so that insect/s could overcome the plant metabolites.
Źródło:: Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm
Autorzy:: Masojc, P
Lapinski, M.
Larsson-Raznikiewicz, M.
Powiązania:: https://bibliotekanauki.pl/articles/2048192.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chromosome
rye
Secale cereale
colorimetric assay
isoenzyme
alpha-amylase
electrophoresis
endosperm
isoelectric focusing
immunoelectrophoresis
cereal cultivar
genetic polymorphism
Opis:: α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.
Źródło:: Journal of Applied Genetics; 1995, 36, 3; 197-204
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Genetic regulation of alpha-amylase synthesis in rye [Secale cereale L.] grain
Autorzy:: Masojc, P
Stojalowski, S
Lapinski, M
Miazga, D
Powiązania:: https://bibliotekanauki.pl/articles/2047279.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: enzyme synthesis
activity
chromosome
rye
Secale cereale
crossing
alpha-amylase
sprouting line
duplicate gene
addition line
genetic regulation
Opis:: Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal addition lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain. The long arm of chromosome 6R was found to be responsible for increased enzyme synthesis during late stages of triticale grain maturation. Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects. Low enzyme activity showed complete dominance over high level of its synthesis. Segregation ratios, observed in F₂ and BC₁ crosses, indicated that recessive alleles at two independent duplicative loci underlie intensive alpha-amylase production.
Źródło:: Journal of Applied Genetics; 1996, 37, 2; 141-152
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
Autorzy:: Sadowski, J
Rorat, T
Cooke, R
Delseny, M
Powiązania:: https://bibliotekanauki.pl/articles/2046684.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction
Opis:: The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Plant height and yield components of inbred isogenic and F1 hybrid Rht dwarf wheats
Autorzy:: Flintham, J E
Gale, M.D.
Powiązania:: https://bibliotekanauki.pl/articles/2044459.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chemical hybridizing agent
F1 hybrid
wheat
plant height
grain yield
hybrid genotype
heterosis
alpha-amylase
enzyme activity
breeding programme
yield component
Rht allele
Opis:: Single and double-gene Rhtl, Rht2, Rht3, Rht1 + Rht2 and Rht3 + Rht2 isogenic lines of wheat in four parental rht varieties were grown in drilled yield trials at four sites in 1989. The same lines were also grown in 1988 together with hybrid genotypes from CHA (chemical hybridising agent) F₁ production plots. In the inbred lines shorter than one metre, Rht alleles reduced total shoot biomass by shortening the straw; mass of straw per unit plant height was unaffected. Highest grain yield was obtained from plant heights between 70 and 100 cm. The Rht genotype achieving this stature varied according to parent varietal height. The hybrids grown allowed comparisons between intra- and inter-varietal crosses over a range of Rht genotypes. In F₁ hybrids positive heterosis was observed for plant height, grain yield and mean grain weight. Highest yields were obtained from inter-varietal hybrids carrying one, two or three doses of Rht1 or Rht2 or one dose of Rht3. An Rht3lrht hybrid showed resistance to premature α-amylase production during grain ripening.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 73-83
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants
Autorzy:: Przybylska, J
Powiązania:: https://bibliotekanauki.pl/articles/2048196.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Pisum
genetic resource
isoenzyme
plant genetics
electrophoresis
leguminous plant
dehydrogenase
taxonomy
albumin
Lupinus
globulin
Phaseolus
peroxidase
Leguminosae
legumin fraction
phaseolin
Papilionoideae
amylase
seed protein
taxonomic investigation
transaminase
protein
aminopeptidase
phenotype
esterase
Opis:: The article reviews the work of the author and coworkers on the use of electrophoretic protein analysis in taxonomie studies and classification of genetic resources of some leguminous plants: the genus Pisum, the Old-World Lupinus species and the cultivated Phaseolus species. On the example of the genus Pisum, the object of many years' investigations, the usefulness of electrophoretic analysis of different protein types - seed globulins, seed albumins and enzyme systems - is critically discussed. It has been concluded that variation in seed albumins, proteins often ignored in comparative analyses, should be more widely considered in taxonomie investigations. The conclusion was supported by the results obtained for Lupinus and Phaseolus species.
Źródło:: Journal of Applied Genetics; 1995, 36, 3; 255-271
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The influence of varying level of wheat proteinaceous inhibitors in wheat-based artificial feed on development parameters of selected stored product insects
Autorzy:: Warchalewski, J R
Gralik, J.
Winiecki, Z.
Nawrot, J.
Piasecka-Kwiatkowska, D.
Powiązania:: https://bibliotekanauki.pl/articles/65768.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: wheat starch
varying level
weevil
plant protection
wheat grain
flour moth
Sitophilus granarius
insect
Tribolium confusum
feed
insect foraging
development parameter
Anagasta kuehniella
feeding intensity
alpha-amylase inhibitor
artificial wheat-based feed
wheat
larval development
flour beetle
artificial feed
granary weevil
Opis:: The artificially made kernels from ground wheat grain, commercial wheat starch and wheat proteinaceous α-amylase inhibitors in different proportions were used as feed for adults of the granary weevil (Sitophilus granarius L.). In the case of larvae of the confused flour beetle (Tribolium confusum Duv.) and the Mediterranean flour moth (Anagasta kuehniella Zell.) the friable feed mixture were used. The survival of S. granarius adults has not been correlated with the soluble proteins extracted from wheat and amylolytic activity located in this protein fraction. On the other hand the weight of dust (the index of feeding intensity) produced during feeding has depended on the presence of α-amylase and trypsin inhibitors in wheat-based feed. A. kuehniella larvae have not developed at all on feed consisted of 50% wheat starch and 50% of crude a-amylase inhibitors from wheat. The same feed has caused 15.1 days of extension in development time of T. confusum larvae. lt attests to specific native enzymatic apparatus existing in alimentary canals of three damaging grain species which can overcome some obstacles even if extremely highly active insect α-amylase inhibitors were present in feed. However, the sufficient nutrient should be available in feed compounds. Nevertheless, some reduction of insects population can be expected.
Sztuczne ziarna zrobione z handlowej skrobii pszennej i białkowych inhibitorów α-amylaz wymieszanych w różnych proporcjach oraz czyste ziarno pszenicy stanowiły pokarm wołka zbożowego (Sitophilus granaris L.). Dla larw trojszyka ulca (Trbolium confusum Duv.) i mklika mącznego (Anagasta kuchniella Zell.) te same produkty podawano w postaci sypkiej. Procent przeżywalności chrząszczy wołka zbożowego nie zależał od zawartości białka rozpuszczalnego ani od aktywności amylolitycznej pokarmu. Z drugiej strony ilość pyłu wytworzonego w czasie żerowania (wskaźnik intensywności żerowania) zależał od obecności inhibitorów α-amylazy i trypsyny. Larwy mklika mącznego nie kończyły rozwoju na pokarmie zawierającym 50% skrobii pszennej i 50 inhibitorów α-amylazy. Ten sam pokarm powodował przedłużenie rozwoju larw trojszyka ulca o 15,1 dnia. To świadczy o istnieniu wrodzonego, specyficznego aparatu enzymatycznego w przewodzie pokarmowym testowanych owadów, który może pokonać nawet ekstremalnie wysoką aktywność inhibitorów α-amylazy obecnych w pokarmie. Efektem działania inhibitorów może być tylko redukcja liczebności populacji.
Źródło:: Journal of Plant Protection Research; 2001, 41, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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