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    Wyświetlanie 1-10 z 10
    Tytuł:
    Molecular characterization and expression analysis of the NLR family CARD containing five transcripts in the pig
    Autorzy:
    Yang, Q.Y.
    Chen, T.
    Chen, Y.B.
    Lan, D.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/32088.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The NOD-like receptor (NLR) family caspase recruitment domain-containing 5 (NLRC5) is one of the newly discovered and largest NLR family members. The NLRC5 has recently received extensive attention because of its important role in regulating innate and adaptive immune responses. The NLRC5 in many vertebrates, such as humans, mice, cattle, and horses, has already been proven and studied. However, the NLRC5 gene characteristics of pigs remain unclear. Thus, we completely cloned the NLRC5 cDNA sequence of the pig using the rapid amplification of cDNA ends(RACE) technology. A characteristic and tissue expression analysis was also conducted on the pig sequence. The sequence analysis showed that the complete cDNA sequence of the NLRC5 of the pig is 6638 bp, and the open reading frame is 5538 bp which encoded 1846 amino acids. The protein prediction analysis indicates that the overall performance of the NLRC5 protein of the pig is hydrophilic and possesses a typical nucleotide binding and oligomerization domain(NBD) and 20 leucine-rich repeats(LRRs). The homology analysis result indicates that the NLRC5 transcript in pigs is highly homologous to cattle, sheep, macaques, and humans, and accounts for around 80%. The genetic evolutionary tree analysis shows that the NLRC5 transcript in pigs has the closest evolutionary relationship with cattle and sheep. Further tissue expression analysis shows that immune organ systems (e.g., lymph node and spleen) and mucosa organs (e.g., intestinal lymph node, stomach, and lungs) possess high expressions with NLRC5 mRNA. The result of this study indicates that the NLRC5 transcript in pigs is relatively conservative among mammals and may play a vital role in immune reaction, which provides a basis for further studies on the NLRC5 function in the pig immune system and the role in comparative immunity.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of seminal plasma concentration on sperm motility and plasma and acrosome membrane integrity in chilled canine spermatozoa
    Autorzy:
    Pan, C.
    Wu, Y.
    Yang, Q.
    Ye, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087767.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    seminal plasma
    chill
    canine
    sperm
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 133-138
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microstructure and Mechanical Behavior of Mg-0.5Si-xSn Alloys
    Autorzy:
    Fu, X.
    Yang, Y.
    Ma, Q. Y.
    Peng, X.
    Xu, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/379670.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    low silicon magnesium alloy
    eutectic Mg2Si
    microstructure behavior
    mechanical behavior
    stop magnezu
    stop Mg2Si
    zachowanie mechaniczne
    Opis:
    Mg-0.5Si-xSn (x=0.95, 2.9, 5.02wt.%) alloys were cast and extruded at 593K (320 ºC) with an extrusion ratio of 25. The microstructure and mechanical properties of as-cast and extruded test alloys were investigated by OM, SEM, XRD and tensile tests. The experimental results indicate that the microstructure of the Mg-0.5Si-xSn alloys consists of primary α-Mg dendrites and an interdendritic eutectic containing α-Mg, Mg2Si and Mg2Sn. There is no coarse primary Mg2Si phase in the test alloys due to low Si content. With the increase in the Sn content, the Mg2Si phase was refined. The shape of Mg2Si phase was changed from branch to short bar, and the size of them were reduced. The ultimate tensile strength and yield strength of Mg-0.52Si-2.9Sn alloy at the temperature of 473K (200 ºC) reach 133MPa and 112MPa respectively. Refined eutectic Mg2Si phase and dispersed Mg2Sn phase with good elevated temperature stability are beneficial to improve the elevated temperature performance of the alloys. However, with the excess addition of Sn, large block-like Mg2Sn appears around the grain boundary leading to lower mechanical properties.
    Źródło:
    Archives of Foundry Engineering; 2017, 17, 4; 179-184
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Design of Acoustic Tubes Array and Application to Measuring Acoustic Loads in Supersonic Airflow
    Autorzy:
    Wei, L.
    Li, M.
    Fu, Q.
    Fan, Y.
    Yang, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/176750.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    acoustic load
    acoustic tubes array
    near field measurement
    supersonic airflow
    Opis:
    In the acoustic fatigue experiment for hypersonic vehicle in simulated harsh service environment on ground, acoustic loads on the surface of test pieces of the vehicle need to be measured. However, for the normal microphones without high temperature resistance ability, the near field sound measurement cannot be achieved. In this work, on the basis of previous researches, an acoustic tubes array is designed to achieve the near field measurement of acoustic loads on the surface of the test piece in the supersonic airflow with high temperature achieved by coherent jet oxygen lance. Firstly, the process of designing this acoustic tubes array is introduced. Secondly, the equality of phase differences at the front and at the end of the tubes is stated and proved using a phase differences test with an acoustic tubes array whose design is presented in this text; therefore, the phase differences of signals acquired by microphones can be directly applied to beamforming algorithm to determine the acoustic load source. Finally, using above mentioned acoustic tubes array, measurement of acoustic load, with and without a test piece in the supersonic airflow made by the coherent jet oxygen lance, is conducted respectively, and the measurements results are analyzed.
    Źródło:
    Archives of Acoustics; 2014, 39, 3; 395-402
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro
    Autorzy:
    Lin, R.
    Wang, Q.
    Qi, B.
    Huang, Y.
    Yang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30295.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GP5 protein - based ELISA for the detection of PRRSV antibodies
    Autorzy:
    Wang, Y.
    Guo, J.
    Qiuao, S.
    Li, Q.
    Yang, J.
    Jin, Q.
    Zhang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30629.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microstructure and Mechanical Properties of Duplex Structured Mg-Li-Zn-Y Alloys
    Autorzy:
    Su, J.
    Yang, Y.
    Fu, X.
    Ma, Q.
    Ren, F.
    Peng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/381787.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alloy Mg-Li-Zn-Y
    microstructure
    mechanical properties
    extruded
    stop Mg-Li-Zn-Y
    mikrostruktura
    właściwości mechaniczne
    wytłaczanie
    Opis:
    As-cast Mg-6Li-0.3Zn-0.6Y and Mg-6Li-1.2Zn-1.2Y (wt%) alloys were prepared and extruded at 260 ºC with an extrusion ratio of 25. The microstructure and mechanical behavior of as-cast and extruded alloys are reported and discussed. The results show that Mg-6Li-1.2Zn- 1.2Y alloy is composed of α-Mg, β-Li, and W-Mg3Zn3Y2 phases while Mg-6Li-0.3Zn-0.6Y alloy contains α-Mg, β-Li, W-Mg3Zn3Y2 phase and X-Mg12ZnY. After hot extrusion, the microstructure of specimens is refined and the average grains size of extruded alloys is 15 μm. Dynamic recrystallization occurs during the extrusion, leading to grain refinement of test alloys. Both the strength and elongation of test alloys are improved by extrusion. The extruded Mg-6Li-0.3Zn-0.6Y alloy possesses an ultimate strength of 225 MPa with an elongation of 18% while the strength and elongation of Mg-6Li-1.2Zn-1.2Y alloy are 206 MPa and 28%, respectively. The X-phase in Mg-6Li-0.3Zn- 0.6Y is beneficial to the improvement of strength, but will lead to the decrease of ductility.
    Źródło:
    Archives of Foundry Engineering; 2018, 18, 1; 181-185
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
    Autorzy:
    Han, K.
    Zhao, D.
    Liu, Y.
    Liu, Q.
    Huang, X.
    Yang, J.
    Bi, K.
    Xu, T.
    Li, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/31495.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
    Autorzy:
    Xu, T.
    Huang, X.
    Li, Y.
    Liu, Q.
    Liu, X.
    Han, K.
    Liu, Y.
    Yang, J.
    Zhao, D.
    Bi, K.
    Sun, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087719.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    goose-derived
    duck-derived
    chicken-derived
    Tembusu virus
    pathogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of synthesis conditions on the synthesis of carbon nanofibers by ethanol catalytic combustion
    Wpływ warunków syntezy na syntezę nanowłókien węglowych metoda katalitycznego spalania etanolu
    Autorzy:
    Cheng, J.
    Zou, X. P.
    Zhu, G.
    Wang, M. F.
    Su, Y.
    Yang, G. Q.
    Lu, X. M.
    Powiązania:
    https://bibliotekanauki.pl/articles/354282.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    synteza
    nanowłókna węglowe
    spalanie
    etanol
    carbon nanofibers
    ethanol catalytic combustion
    influence factors
    Opis:
    In this paper, the effects of position of substrates in flames, preparation time, stability of flames and catalyst precursors on the synthesis of carbon nanofibers (CNFs) by ethanol catalytic combustion (ECC) were investigated. For investigating the effects of these influence factors on the synthesis of CNFs, several sets of controlled experiments were performed, such as preparation experiments with different position of substrates in flames, different preparation time, stable and unstable flames, and different catalyst precursors. In our experiments, the catalyst precursors were iron nitrate, cobalt nitrate, nickel nitrate, and iron chloride, cobalt chloride, nickel chloride. The as-synthesized products were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy. Our results indicate that the optimal position of substrates in flames is more than 1cm and less than 2.5cm, the optimal preparation time is more than 5min and less than 30min for massive yield, stable flames would be tent to synthesize CNFs with mainly single-type morphology and could improve the graphitization of CNFs, and the catalyst precursors obviously have effects on the synthesis of CNFs.
    W niniejszej pracy zbadany został wpływ położenia substratów w płomieniach, czasu przygotowania, stabilności płomieni i prekursorów katalizatora na syntezę nanowłókien węglowych (CNFs) metodaą katalitycznego spalania etanolu (ECC). W celu zbadania wpływu tych czynników na syntezę nanowłókien węglowych przeprowadzono kilka zestawów kontrolowanych eksperymentów, przy różnej pozycji podłoży w płomieniach, różnym czasie przygotowania, płomieniach stabilnych i niestabilnych i przy użyciu różnych prekursorów katalizatora. W naszych eksperymentach jako prekursory katalizatora stosowano azotany: żelaza, kobaltu i niklu oraz chlorki żelaza, kobaltu i niklu. Tak otrzymane produkty syntezy scharakteryzowano za pomocą skaningowej mikroskopii elektronowej (SEM), transmisyjnej mikroskopii elektronowej (TEM) i spektroskopii Raman'a. Nasze wyniki wskazują, że optymalna pozycja podłoży w płomieniach to więcej niż 1 cm i mniej niż 2,5 cm, a optymalny czas przygotowania to więcej niż 5 min i mniej niż 30 min dla dużej wydajności. Stabilność płomieni będzie sprzyjać syntezie nanowłókien o morfologii jednego typu i może poprawić ich grafityzacje, a rodzaj prekursora katalizatora ma wpływ na syntezę nanowłókien.
    Źródło:
    Archives of Metallurgy and Materials; 2012, 57, 3; 745-751
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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