- Tytuł:
- Sucrose and cytokinin interactions in relation to ethylene and abscisic acid production in the regulation of morphogenesis in pelargonium x hortorum L.H. Bailey in vitro
- Autorzy:
-
Wojtania, A.
Wegrzynowicz-Lesiak, E.
Dziurka, M.
Waligorski, P. - Powiązania:
- https://bibliotekanauki.pl/articles/19900.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Opis:
- The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L-1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO3), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L-1) in the presence of mT and AgNO3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L-1) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.
- Źródło:
-
Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 1
0001-5296 - Pojawia się w:
- Acta Biologica Cracoviensia. Series Botanica
- Dostawca treści:
- Biblioteka Nauki
Artykuł