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    Tytuł:
    Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland
    Autorzy:
    Stepniewska, K.
    Urbaniak, K.
    Markowska-Daniel, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/31229.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    A total of 209 Bordetella bronchiseptica (Bbr) strains isolated from pigs were examined. Phenotypic study included: biochemical characterization (motility, catalase, oxidase, urease activity, nitrate reduction and growth on MacConkey agar) and antimicrobial susceptibility (disc diffusion method). Genotypic studies based on detection of three genes encoded virulence factors, such as: flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. Most of the Bbr strains tested had a homogeneous biochemical profile. 97.6% of them provided suitable results in biochemical tests. All Bbr isolates tested showed high resistance to penicillin (100%), linco-spectin (100%) and ceftiofur (97.9%). Over 57% and 43% of Bbr strains were resistant to ampicillin and amoxicillin, respectively. All Bbr isolates showed high sensitivity to most chemotherapeutics used such as enrofloxacin (97.9%), tetracycline (97.9%), oxytetracycline (97.9%), amoxicillin with clavulonic acid (95.8%), florfenicol (90.4%), and gentamicine (77.6%). Over of 94% of Bbr strains were moderately susceptible to norfloxacine. Molecular analysis confirmed that almost all evaluated Bbr strains (94.7%) possessed the fla gene. A lower percentage of isolates had the dnt gene (72.7%) and the lowest percentage of strains (51.7%), had the bfrZ gene.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008
    Autorzy:
    Markowska-Daniel, I
    Urbaniak, K.
    Stepniewska, K.
    Pejsak, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/30384.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Polska
    pig
    respiratory tract
    bacteria
    isolation
    antibiotic susceptibility
    animal health
    productivity
    animal production
    Opis:
    Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 29-36
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Low-Temperature Plasma Treatment on The Liquid Filtration Efficiency of Melt-Blown PP Nonwovens in The Conditions of Simulated Use of Respiratory Protective Equipment
    Autorzy:
    Majchrzycka, K.
    Okrasa, M.
    Brochocka, A.
    Urbaniak-Domagała, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/185013.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    low-temperature plasma treatment
    melt-blown nonwovens
    respiratory protective
    devices
    obróbka plazmowa w niskich tepmeraturach
    Opis:
    Filtering nonwovens produced with melt-blown technology are one of the most basic materials used in the construction of respiratory protective equipment (RPE) against harmful aerosols, including bio- and nanoaerosols. The improvement of their filtering properties can be achieved by the development of quasi-permanent electric charge on the fibres. Usually corona discharge method is utilized for this purpose. In the presented study, it was assumed that the low-temperature plasma treatment could be applied as an alternative method for the manufacturing of conventional electret nonwovens for the RPE construction. Low temperature plasma treatment of polypropylene nonwovens was carried out with various process gases (argon, nitrogen, oxygen or air) in a wide range of process parameters (gas flow velocity, time of treatment and power supplied to the reactor electrodes). After the modification, nonwovens were evaluated in terms of filtration efficiency of paraffin oil mist. The stability of the modification results was tested after 12 months of storage and after conditioning at elevated temperature and relative humidity conditions. Moreover, scanning electron microscopy and ATR-IR spectroscopy were used to assess changes in surface topography and chemical composition of the fibres. The modification of melt-blown nonwovens with nitrogen, oxygen and air plasma did not result in a satisfactory improvement of the filtration efficiency. In case of argon plasma treatment, up to 82% increase of filtration efficiency of paraffin oil mist was observed in relation to untreated samples. This effect was stable after 12 months of storage in normal conditions and after thermal conditioning in (70 ± 3)°C for 24 h. The use of low-temperature plasma treatment was proven to be a promising improvement direction of filtering properties of nonwovens used for the protection of respiratory tract against harmful aerosols.
    Źródło:
    Chemical and Process Engineering; 2017, 38, 2; 195-207
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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