Autor: Rola, K. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Cell pattern and ultrasculpture of bulb tunics of selected Allium species (Amaryllidaceae) and their diagnostic value
Autorzy:: Rola, K.
Powiązania:: https://bibliotekanauki.pl/articles/19259.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2014, 56, 1
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Intraspecific molecular variation of Allium ursinum (Amaryllidaceae) across the border of two subspecies distribution ranges
Autorzy:: Rola, K.
Lenart-Boron, A.
Boron, P.
Osyczka, P.
Powiązania:: https://bibliotekanauki.pl/articles/19331.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: The study investigates the genetic differentiation among two subspecies of Allium ursinum L., namely A. ursinum subsp. ursinum and subsp. ucrainicum as well as their putative hybrid that is represented by individuals with intermediate morphology. Inter-Simple Sequence Repeats (ISSR) were applied to determine the status of intermediate morphotypes in terms of their genetic pattern and to assess the level of genetic variability within and between various populations of A. ursinum. The study comprises 144 specimens from nine populations along the east-west transect in Poland, which includes localities of both subspecies and their putative hybrid. Among the examined populations, 48 bands were amplified, of which 45 were found to be polymorphic. The principal coordinate analysis (PCoA), the neighbour-net analysis and Mantel test showed a strong correlation between genetic variability and geographic distance. Analysis of molecular variance (AMOVA) revealed that a greater proportion of total genetic variation resided within populations rather than among them. The Structure Bayesian clustering analysis revealed the presence of three distinct genetic groups within studied populations, where 'eastern' genotypes correspond to A. ursinum subsp. ucrainicum, and 'western' to subsp. ursinum; whereas the third genetic group has the largest share in the individuals occurring at the border of the distribution ranges of both subspecies. The emergence of the third genetic group is probably an effect of hybridization events occurring within the secondary contact zone. Typical morphologically intermediate populations occur only in a relatively narrow geographical zone, but the hybrid zone revealed by molecular markers is actually much wider than it is suggested by the morphological pattern of individuals. The current distribution pattern of both subspecies of A. ursinum and their hybrid zone is related to the two main directions of postglacial migration of Fagus sylvatica to the area of Poland. The hybrid zone arose as an effect of the secondary contact of two divergent lineages of A. ursinum.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 1
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: The possibility of weed control in cereals by use low rates of herbicides - review of existing investigations
Autorzy:: Domaradzki, K.
Rola, H.
Powiązania:: https://bibliotekanauki.pl/articles/66498.pdf
Data publikacji:: 2003
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: investigation
regulation
weed infestation
herbicide
cereal
weed control
possibility
reduced dose
Źródło:: Journal of Plant Protection Research; 2003, 43, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Application of real - time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses
Autorzy:: Stasiak, K.
Rola, J.
Zmudzinski, K.
Powiązania:: https://bibliotekanauki.pl/articles/32465.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0x100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real- time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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