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    Wyświetlanie 1-10 z 10
    Tytuł:
    Experimental and numerical study of the uniaxial compressive stress-strain relationship of a rock mass with two parallel joints
    Autorzy:
    Xiong, L. X.
    Yuan, H. Y.
    Zhang, Y.
    Zhang, K. F.
    Li, J. B.
    Powiązania:
    https://bibliotekanauki.pl/articles/231076.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    masa skalna łączona
    złącze równoległe
    siła szczytowa
    jointed rock mass
    parallel joint
    peak strength
    Opis:
    A “rock bridge”, defined as the closest distance between two joints in a rock mass, is an important feature affecting the jointed rock mass strength. Artificial jointed rock specimens with two parallel joint fractures were tested under uniaxial compression and numerical simulations were carried out to study the effects of the inclination of the rock bridge, the dip angle of the joint, rock bridge length, and the length of joints on the strength of the jointed rock mass. Research results show: (1) When the length of the joint fracture, the length of the rock bridge, and the inclination of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually increases as the inclination of the joint fracture increases from 0° to 90°. (2) When the length of the joint fracture, the length of the rock bridge, and the inclination of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen shows variations in trends with the inclination of the rock bridge increasing from 30° to 150° (3). In the case when the joint is angled from the vertical loading direction, when the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually decreases with an increasing length of joint fracture. When the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen does not show a clear trend with an increase of the length of the rock bridge.
    Źródło:
    Archives of Civil Engineering; 2019, 65, 2; 67-80
    1230-2945
    Pojawia się w:
    Archives of Civil Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ISSR analysis of somaclonal variation in callus-derived plants of Amorphophallus rivieri Durieu
    Autorzy:
    Hu, J.B.
    Li, Q.
    Li, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/19740.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    somaclonal variation
    callus-derived plant
    Amorphophallus rivieri
    ISSR technique
    regenerated plant
    plant cultivation
    crop
    China
    Opis:
    This communication reports detection of somaclonal variation among tissue culture-raised plants of Amorphophallus rivieri Durieu, an economically important crop in China, with high content of glucomannan in its corms. A population of regenerated plants was obtained from a single donor plant of A. rivieri via corm organogenesis, and 28 plants were randomly selected as a representative sample and subjected to analysis of somaclonal variation using inter-simple sequence repeat (ISSR) markers. Of the 26 ISSR primers screened, 13 gave distinct and reproducible band patterns, yielding 131 bands with an average of 10.1 bands per primer. Ten primers were polymorphic and generated 16 polymorphic bands with 12.2% mean polymorphism. Based on the ISSR data from the regenerated plants and the donor plant, Jaccard's similarity coefficients were calculated; they ranged from 0.961 to 1.000 with a mean of 0.982. A dendrogram was constructed using the unweighted pair group method with arithmetic mean (UPGMA); it showed that a majority of regenerated plants (including the donor plant) clustered closely, with a mean similarity coefficient of 0.987. Low somaclonal variation observed in the regenerated plants indicates that rapid propagation of A. rivieri via corm organogenesis is a practicable method with a low risk of genetic instability.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of the influence on expressway safety of ramps
    Autorzy:
    Hu, J. J.
    Li, F.
    Han, B.
    Yao, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/224137.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    expressway safety
    safety
    on and off ramp
    delay
    accidents occurrence probability
    droga ekspresowa
    bezpieczeństwo na drodze ekspresowej
    rampa włączania i wyłączania
    opóźnienie
    prawdopodobieństwo wystąpienia awarii
    Opis:
    As the very important parts of the expressway system, on and off ramps have a great effect on the operation effect of expressways. Once congestion occurs at on and off ramps, it will directly affect the safety of the expressway and vehicles. So this paper first analyzes the location design of on ramps, the length and the influences on the expressway running state of off ramp downstream traffic state, and establishes the expressway operation models based at on and off ramps. The expressway operation models include off ramp delay model, on ramp delay model, expressway delay model and side road delay model under the influence of the distance between on and off ramp and traffic volume. At the same time, the paper analyzes the connection between the traffic operation state and traffic safety, and establishes the expressway safety model based on congestion degree. Through simulation verification, the impact of on and off ramp on the security of the expressway is analyzed. In the simulation, safety rank division is simulated to verify and safety index of different safety ranks. Finally the paper concludes that the delay caused by traffic of the on and off ramps can decrease the safety of expressway.
    Źródło:
    Archives of Transport; 2017, 43, 3; 43-51
    0866-9546
    2300-8830
    Pojawia się w:
    Archives of Transport
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modulation of virulence genes by the two-component system PhoP - PhoQ in avian pathogenic Escherichia coli
    Autorzy:
    Tu, J.
    Huang, B.
    Zhang, Y.
    Xue, T.
    Li, S.
    Qi, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30418.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes. We first confirmed that AE18, AE19, and AE20 were successfully constructed from the wild-type AE17 strain. AE18, AE19, and AE20 showed significant decreases in motility of 70.97%, 83.87%, and 37.1%, respectively, in comparison with AE17. Moreover, in comparison with AE17, AE18, AE19, and AE20 showed significant decreases of 63.11%, 65.42%, and 30.26%, respectively, in CEF cell adhesion, and significant decreases of 59.83%, 57.82%, and 37.90%, respectively, in CEF cell invasion. In comparison with AE17, transcript levels of sodA, polA, and iss were significantly decreased in AE18, while transcript levels of fimC and iss were significantly decreased in AE19. Our results demonstrate that deletion of PhoP or PhoQ inhibits invasion and adhesion of APEC to CEF cells and significantly reduces APEC virulence by regulating transcription of virulence genes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pharmacokinetics of the recombinant ovine interferon-tau in lambs
    Autorzy:
    Zhao, J.
    Yu, H.Y.
    Zhao, Y.
    Li, S.Q.
    Fu, X.L.
    Zhou, W.
    Xia, B.B.
    Wang, M.L.
    Chen, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087588.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bioavailability
    ovine interferon-τ
    cytopathic effect inhibition assay
    pharmacokinetic study
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 75-82
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of Sulfur and Acid Soluble Aluminum Content on Precipitate and Microstructure of Grain-Oriented Silicon Steel
    Autorzy:
    Zhou, B.
    Zhu, C.
    Li, G.
    Luo, Z.
    Gao, Y.
    Bao, S.
    Schneider, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/354451.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    grain oriented silicon steel
    sulfur
    acid soluble aluminum
    precipitate
    microstructure
    texture
    Opis:
    The combined effect of sulfur (S) and acid soluble aluminum (Als ) content on precipitates and microstructures in grain-oriented silicon steel were investigated. The results show that there are dominant AlN and a little amount of MnS-AlN composite in annealed hot-rolled band, and the amount of precipitates increases distinctly with increasing Als content, while S content plays a negligible role. The inhibitors that precipitate during hot band annealing can restrain the grain growth during hot band annealing and primary annealing, and the smaller grains of annealed hot-rolled band can contribute to the formation of {111} <112> texture during primary annealing. Lower S content is conducive to the formation of {111} <112> texture during primary annealing by promoting the formation of Goss texture during hot rolling.
    Źródło:
    Archives of Metallurgy and Materials; 2018, 63, 4; 1701-1708
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Vertical transmission of PCV2b to fetuses in sows intramuscularly infected with PCV2b
    Autorzy:
    Wang, N.D.
    Li, J.J.
    Wang, A.B.
    Zhu, Z.
    Chang, Y.
    Mao, Y.
    Zan, Y.
    Wang, Z.F.
    Deng, Z.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31535.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In order to investigate whether PCV2b can be transmitted across the placenta in sows thereby causing vertical infection of fetuses, six sows in 75 day of pregnancy were either intramuscularly inoculated with a PCV2b culture supernatant (n = 4) or mock infected with cell culture supernatant (n = 2). At parturition, 3 newborn piglets from each litter were randomly selected and euthanatized prior to suckling. Samples of the mesenteric lymph nodes, spleens, kidneys, hearts and lungs were collected for PCR, histopathological and immunohistochemical assays. The results showed that the newborn piglets from PCV2b-infected sows had histopathological lesions in the tested tissues. Moreover, PCV2b antigen and DNA were detected as well. These findings therefore suggested that porcine circovirus type 2b can be transmitted across the placenta of sows, thereby leading to PCV2b vertical infection of the fetuses.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
    Autorzy:
    Hiszczynska-Sawicka, E.
    Li, H.
    Xu, J.B.
    Akhtar, M.
    Holec-Gasior, L.
    Kur, J.
    Bickerstaffe, R.
    Stankiewicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30828.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    immune response
    sheep
    vaccination
    Toxoplasma gondii
    parasite
    protozoan parasite
    pregnancy
    MIC3 gene
    MIC3 antigen
    Opis:
    Toxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control its adverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii. The viability of MIC3 as a vaccine is investigated in this study. Sheep were injected twice, intramuscularly, with plasmids containing DNA encoding for the mature form of MIC3 protein formulated into liposomes. Control sheep were injected with an empty vector or received no injections. The injection of sheep with DNA plasmids encoding for MIC3 elicited an immune response after the first and second injections as indicated by antibody responses and the production of IFN-γ. The immune response, as measured by the IgG2 and IgG1 serum levels, was boosted after the injection of the MIC3 DNA vaccine together with high anti-MIC3 antibodies. The results demonstrate that the intramuscular injection of sheep with a plasmid containing DNA coding for MIC3 protein induces a significant and effective immune response against T. gondii.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
    Autorzy:
    Wang, Y.B.
    Li, Y.H.
    Li, Q.M.
    Xie, W.T.
    Guo, C.L.
    Guo, J.Q.
    Deng, R.G.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087396.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pseudorabies virus variant strains
    anti-pseudorabies virus monoclonal antibody
    blocking immunoperoxidase monolayer assay
    differentiation between pseudorabies virus-infected and vaccinated animals
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 4; 717-723
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
    Autorzy:
    Sun, P.
    Bai, C.X.
    Zhang, D.
    Wang, J.
    Yang, K.K.
    Cheng, B.Z.
    Li, Y.D.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087328.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    porcine parvovirus 6
    real-time polymerase chain reaction
    SYBR Green
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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