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    Wyświetlanie 1-3 z 3
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Generalized route planning approach for hazardous materials transportation with equity consideration
    Autorzy:
    Chai, H.
    He, R.-C.
    Jia, X.-yan
    Ma, Ch.-x
    Dai, C.-jie
    Powiązania:
    https://bibliotekanauki.pl/articles/223759.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    hazardous materials transportation
    transportation
    route optimization
    risk equity
    multi-objective optimization
    NSGA-II algorithm
    genetic algorithm
    transport materiałów niebezpiecznych
    materiały niebezpieczne
    optymalizacja trasy
    kapitał własny
    optymalizacja wielokryterialna
    algorytm NSGA-II
    algorytm genetyczny
    Opis:
    Hazardous materials transportation should consider risk equity and transportation risk and cost. In the hazardous materials transportation process, we consider risk equity as an important condition in optimizing vehicle routing for the long-term transport of hazardous materials between single or multiple origin-destination pairs (O-D) to reduce the distribution difference of hazardous materials transportation risk over populated areas. First, a risk equity evaluation scheme is proposed to reflect the risk difference among the areas. The evaluation scheme uses standard deviation to measure the risk differences among populated areas. Second, a risk distribution equity model is proposed to decrease the risk difference among populated areas by adjusting the path frequency between O-D pairs for hazardous materials transportation. The model is converted into two sub models to facilitate decision-making, and an algorithm is provided for each sub model. Finally, we design a numerical example to verify the accuracy and rationality of the model and algorithm. The numerical example shows that the proposed model is essential and feasible for reducing the complexity and increasing the portability of the transportation process.
    Źródło:
    Archives of Transport; 2018, 46, 2; 33-46
    0866-9546
    2300-8830
    Pojawia się w:
    Archives of Transport
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Research on Characterization of Crushability for Foundry Sand Particles
    Autorzy:
    Dai, Y.
    Ma, Q. Y.
    Li, X. H.
    Zhang, X.
    Hu, F. P.
    Zhang, Y.
    Xie, W. D.
    Powiązania:
    https://bibliotekanauki.pl/articles/379689.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    foundry sand particle
    mechanical load
    AFS grain fineness
    crushability
    piasek odlewniczy
    obciążenie mechaniczne
    rozdrobnienie ziarna
    Opis:
    In this paper, crushability of foundry sand particles was studied. Three kinds of in-service silica sands in foundry enterprises selected as the study object, and foundry sand particles were subjected to mechanical load and thermal load during service were analyzed. A set of methods for simulating mechanical load and thermal load by milling and thermal-cold cycling were designed and researched, which were used to characterize the crushability for silica sand particles, the microstructure was observed by SEM. According to the user’s experience in actual application, the crushability of Sand C was the best and then Sand B, the last Sand A. The results indicated that mechanical load, thermal load and thermal-mechanical load can all be used to characterize the crushability of foundry sand particles. Microscopic appearances can qualitatively characterize the crushability of foundry sand particles to a certain extent, combining with the additions and cracks which are observed on the surface.
    Źródło:
    Archives of Foundry Engineering; 2017, 17, 4; 231-235
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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