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Wyświetlanie 1-15 z 34
Tytuł:
Protein patterns and their association with photosynthetic pigment content, agronomic behavior, and origin of purslane accessions (Portulaca oleracea L.)
Autorzy:
Talei, D.
Naji, A.M.
Powiązania:
https://bibliotekanauki.pl/articles/2096425.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chlorophyll
genetic diversity
morphometric
Portulaca oleracea
protein pattern
Opis:
In this study, the proteomic, morphometric, and photosynthetic pigment data of purslane (Portulaca oleracea) accessions were combined together to show their impact on genetic variation in order to establish a relationship between protein patterns and phenotypic behavior of the plant. Seeds of 18 collected purslane accessions were cultivated based on a completely randomized design with three replicates. Before the flowering stage, the data on morphology, photosynthetic pigment content, and seed proteins were obtained. The results showed a significant difference among purslane accessions in terms of the most studied agronomic characteristics and the content of photosynthetic pigments and proteins. The cluster analysis of the 18 purslane accessions based on agronomic data, and photosynthetic pigment content, and protein pattern data produced three main clusters. Moreover, the seed protein analysis revealed that the two polymorphic protein bands of size 40 kDa (protein “a”) and 30 kDa (protein “b”) effectively diversified the agronomic, photosynthetic pigment, and phylogenetic relationships among the purslane accessions. Interestingly, protein “a” was produced in plants growing in low altitude areas and played a suppressive role for TDW, while protein “b” was produced in plants growing in high altitude areas and functioned as an activator agent for this trait. Overall, the outcomes of the present study indicated the presence of high genetic variability (77.6%) among the purslane accessions. These findings suggest that these proteins should be sequenced for further proteomic analyses and can be used for hybridization to generate useful recombinants in segregating generations and improve breeding varieties of P. oleracea.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 245-255
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical evaluation of expression of heat shock proteins HSP70 and HSP90 in mammary gland neoplasms in bitches
Autorzy:
Badowska-Kozakiewicz, A.M.
Malicka, E.
Powiązania:
https://bibliotekanauki.pl/articles/31330.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Heat shock proteins have essential roles in a number of pathophysiologic conditions including carcinogenesis and represent a group of novel molecular markers in cancer management. The aim of this study was to investigate heat shock protein expression in correlation with other neoplasm traits such as: histological type, differentiation grade, proliferative activity, estrogenic receptor expression, and cyclooxygenase-2 and p53 proteins. Material for the investigation comprised 133 tumors of the mammary gland collected from bitches. In total 14 adenomas, 66 complex carcinomas, 47 simple carcinomas and 6 solid carcinomas were collected. Evaluations were conducted with histopathological and immunohistochemical methods using suitable antibodies. Expression of heat shock protein 70 was observed in all types of evaluated neoplasms. A higher average number of cells undergoing expression of heat shock protein 70, which was statistically insignificant, was established in complex and simple cancers and in cancers with the 1st and the 2nd degree of histological malignancy. Expression of heat shock protein 90 was observed in all studied neoplasms; it was very insignificant in adenomas, compared to cancers, and the highest expression was established in the solid cancers, as well as in cancers with the 2nd degree of histological malignancy. This high expression of heat shock protein 90 was correlated with proliferative activity. The results suggest that heat shock protein 90 is involved in canine mammary gland carcinogenesis. The results also suggest that heat shock protein 90 may be a prognostic factor, but this requires detailed clinical confirmation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induced systemic resistance to wheat take-all disease by probiotic bacteria
Autorzy:
Jasem, A.M.
Sharifi, R.
Abbasi, S.
Powiązania:
https://bibliotekanauki.pl/articles/65078.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant disease
soil-borne disease
Gaeumannomyces tritici
wheat yield
induced systemic resistance
probiotic bacteria
methyl jasmonate
methyl salicylate
rhizobacteria
Opis:
In this study, the effect of six commercial biocontrol strains, Bacillus pumilus INR7, B. megaterium P2, B. subtilis GB03, B. subtilis S, B. subtilis AS and B. subtilis BS and four indigenous strains Achromobacter sp. B124, Pseudomonas geniculate B19, Serratia marcescens B29 and B. simplex B21 and two plant defense inducers, methyl salicylate (Me-SA) and methyl jasmonate (Me-JA) were assessed on suppression of wheat take-all disease. Treatments were applied either as soil drench or sprayed on shoots. In the soil drench method, the highest disease suppression was achieved in treatment with strains INR7, GB03, B19 and AS along with two chemical inducers. Bacillus subtilis S, as the worst treatment, suppressed take-all severity up to 56%. Both chemical inducers and bacterial strains AS and P2 exhibited the highest effect on suppression of take-all disease in the shoot spray method. Bacillus subtilis S suppressed the disease severity up to 49% and was again the worst strain. The efficacy of strains GB03 and B19 decreased significantly in the shoot spray method compared to the soil drench application method. Our results showed that most treatments had the same effect on take-all disease when they were applied as soil drench or sprayed on aerial parts. This means that induction of plant defense was the main mechanism in suppressing take-all disease by the given rhizobacteria. It also revealed that plant growth was reduced when it was treated with chemical inducers. In contrast, rhizobacteria not only suppressed the disease, but also increased plant growth.
Źródło:
Journal of Plant Protection Research; 2018, 58, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of methods for the detection of low-abundant snoRNA-derived small RNAs in Saccharomyces cerevisiae
Autorzy:
Walkowiak, M.
Mleczko, A.M.
Bakowska-Zywicka, K.
Powiązania:
https://bibliotekanauki.pl/articles/80556.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2016, 97, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecural identification of Fascioloides magna (Bassi, 1875) from red deer from South-Western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2)
Autorzy:
Pyziel, A.M.
Demiaszkiewicz, A.W.
Kuligowska, I.
Powiązania:
https://bibliotekanauki.pl/articles/30926.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The study was conducted in 2012-2013 on 75 fecal samples of red deer from the Lower Silesian Wilderness which were examined to determine the prevalence of Fascioloides magna in the game population. Finding liver fluke eggs in a single sample which were larger in size than Fasciola hepatica eggs indicated that further molecular analysis was necessarily. The partial sequence (116 bp long) of ITS-2 of the investigated eggs was identical to the sequences of F. magna from red deer (Cervus elaphus) (GenBank, EF534993; GenBank, EF534992) and from wapiti deer (Cervus elaphus canadensis) (GenBank, EF534994) from Slovakia, as well as from fallow deer (Dama dama) from the USA (GenBank, EF051080). This is the first molecular confirmation of the occurrence of F. magna in Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Feline ocular toxoplasmosis: seroprevalence, diagnosis and treatment outcome of 60 clinical cases
Autorzy:
Ali, K.M.
Abu-Seida, A.M.
Abuowarda, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087214.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cats
chorioretinitis
keratitis
seroprevalence
Toxoplasma gondii
uveitis
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 51-61
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The application of in vitro cattle embryo production system to study the influence of elevated temperature on oocyte maturation, fertilization and early embryonic development
Autorzy:
Rynkowska, A.
Rapala, L.
Trzeciak, P.
Duszewska, A.M.
Powiązania:
https://bibliotekanauki.pl/articles/80225.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidant
apoptosis
cattle
embryo
embryonic development
female reproductive system
fertilization
glutathione
heat shock protein
Hsp70 protein
hyperthermia
in vitro
oocyte
oocyte maturation
sperm
temperature
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Life with oxidative stress
Autorzy:
Gurda, D.
Kietrys, A.M.
Szopa, A.
Twardowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/185377.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ROS
oxidative stress
antioxidants
oxidative stress response
stres oksydacyjny
przeciwutleniacze
reakcja na stres oksydacyjny
Opis:
Incomplete oxygen reduction gives rise to reactive oxygen species (ROS). For a long time they have been considered unwelcome companions of aerobic metabolism. Organisms using oxygen developed several systems of ROS scavenging with enzymatic and non enzymatic antioxidants, which allow them control the cellular level of oxygen derived from free radicals. It is well established nowadays that ROS are not necessarily negative byproducts, but they also play an important role in cellular mechanisms. They are involved in many regular cellular processes in all aerobic organisms. When the antioxidant system is overcome and the balance between ROS production and scavenging is disrupted, oxidative stress occurs. It has been reported that oxidative stress may be linked to some human diseases and is also involved in biotic and abiotic stress response in plants.
Źródło:
Chemical and Process Engineering; 2012, 33, 4; 509-528
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Decrease of the herbicide fenoxaprop phytotoxicity in drought conditions: the role of the antioxidant enzymatic system
Autorzy:
Radchenko, M.P.
Sychuk, A.M.
Morderer, Y.Y.
Powiązania:
https://bibliotekanauki.pl/articles/66477.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This study investigated the effects of the herbicide from the graminicide group fenoxaprop, on oat plants under a normal and reduced water supply. The study was done in vegetative experimental conditions. It was established, that plants treated with fenoxaprop after 2 days following a reduction of the water content in soil with 60% to 40% of full field moisture capacity, significantly reduced phytotoxic action. At the decreased level of water supply in the oat plants, activity of antioxidant enzymes – superoxide dismutase, and catalase increased. The fenoxaprop action did not lead to a substantial increase in superoxide anion radical and hydrogen peroxide content, which had been observed under the action of the herbicide when normal levels of water were supplied to the plants. It was concluded, that the phytotoxic action of the herbicides from the graminicide group mediated the formation of reactive oxygen species and reduction of phytotoxicity on the background effect on plants of various stressors. This reaction was due to the increased activity of antioxidant enzymes, associated with nonspecific reaction of plants to these stressors.
Źródło:
Journal of Plant Protection Research; 2014, 54, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Echinochloa colonum resistance to bispyribac-soduim in Egypt - occurrence and identification
Autorzy:
El-Nady, M.F.
Hamza, A.M.
Derbalah, A.S.
Powiązania:
https://bibliotekanauki.pl/articles/65646.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Identification and mechanism of Echinochloa colonum (L.) resistance to bispyribac-soduim via physiological and anatomical differences between susceptible and resistant biotypes was investigated. The physiological and anatomical differences that were take into account were growth reduction, chlorophyll content reduction, protein analysis, lamina thickness and xylem vessel diameter in both susceptible and resistant biotypes of E. colonum. The results showed the growth reduction fifty (GR50) of resistant biotype was 10.2 times higher than that of the susceptible biotype E. colonum treated with bispyribac-soduim. The chlorophyll content was highly reduced in the susceptible biotype relative to the resistant one of E. colonum treated with bispyribac-soduim. An anatomical test showed significant differences in the cytology of susceptible and resistant biotypes of E. colonum treated with bispyribac-soduim with respect to lamina thickness and xylem vessel diameter. Furthermore, leaf protein analysis showed significant differences between the susceptible and resistant biotypes of E. colonum in the number and the density of protein bands. The resistance of E. colonum to bispyribac-soduim may be due to the faster metabolism of bispyribac-soduim below the physiologically active concentration or the insensitivity of its target enzyme, (acetolactate synthase). These results implied the occurrence of E. colonum resistance to bispyribac-soduim in Egypt and provide conclusive evidence that a single resistance mechanism alone cannot explain insensitivity in E. colonum to bispyribac-soduim.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipid production and heavy metals adsorption by an Aspergillus fumigatus GAH1 isolate
Autorzy:
Haddad, A.M.
El-Shall, H.S.
Abu-Elreesh, G.
Powiązania:
https://bibliotekanauki.pl/articles/2096970.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lipids
biodiesel
fungi
Aspergillus
bioremediation
heavy metals
Opis:
Few species of filamentous fungi can accumulate considerable amounts of intracellular lipids when grown under certain stress conditions; thus, they can be used for biodiesel production. Moreover, their residual biomass can be used for the removal of heavy metals from contaminated water. An oleaginous Aspergillus fumigatus GAH1 isolate was isolated from a soil sample in Borg El-Arab industrial area, Alexandria, Egypt, and was characterized in batch cultures. The influence of different carbon and nitrogen sources and the initial pH of the medium on the fungal biomass and lipid production was investigated. Under optimized conditions, the A. fumigatus GAH1 isolate could accumulate up to 22.4% of its dry biomass as lipids. Oleic and palmitic acids were the dominant fatty acid fractions produced. After lipid extraction, to further improve the process economy, the fungal biomass was applied as a biosorbent to remove heavy metals such as Cr, Cd, and Zn from contaminated wastewater. The biosorption efficiency and capacity of the raw, H2O-washed, and NaOH-treated fungal biomass were examined. The biosorption capacity of 33.37, 29.29, and 27.75 mg/g was recorded for Cr, Cd, and Zn, respectively. Moreover, the biosorption efficiency was more than 69% for the tested heavy metals. In conclusion, A. fumigatus GAH1 may be considered as a promising biodiesel producer, and its biomass waste can be further used for the biosorption of heavy metals from contaminated wastewater.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 15-24
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening and identification of novel cellulolytic Trichoderma species from Egyptian habitats
Autorzy:
Hewedy, O.A.
El-Zanaty, A.M.
Fahmi, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/2097005.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Trichoderma
cellulase activity
biodegradation
ITS
tef1
Opis:
The present study aimed to isolate indigenous Trichoderma fungi possessing cellulolytic activities from different geographical locations in Egypt. Such strains can be used to convert crop residues to bioproducts, which presently is one of the most important issues in developing countries. The isolates were identified using molecular approaches and morphological characteristics. Favorable growth conditions for Trichoderma isolates (media & temperature) were assessed in vitro. The growth rate of Trichoderma isolates was assessed on two media, namely Potato Dextrose Agar (PDA) and Synthetic Nutrient Agar (SNA), and morphological parameters, i.e., shape, pigment, and edge were observed. A comparison of sequences encoding for internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1 ) was conducted to determine the species. Cellulase assay was performed using different cellulose substrates (Swollen, CMC (low viscosity carboxymethylcellulose), and Avicel). Among the isolated fungi, Trichoderma viride EGY-T27 had the highest endoglucanase activity, while Trichoderma harzianum EGY-T30 showed the highest exoglucanase activity. A total of 27 strains of Trichoderma were identified using ITS, and the tef1 gene was sequenced for 23 strains. The tested strains were identified with acceptable quality based on ITS sequences, which ranged from 98 to 100%, except for strain MH908499 (85%). This research constitutes the most comprehensive study on screening for fungal strains with the highest biodegradable potential isolated from various locations in Egypt.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 117-133
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of sterile wild oat (Avena sterilis L.) resistance to acetolactate synthase (ALS)-inhibiting herbicides using different assay techniques
Autorzy:
Abdurruhman, A.M.
Uygur, S.
Mathiassen, S.K.
Uygur, N.
Powiązania:
https://bibliotekanauki.pl/articles/2082753.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
acetolactate synthase
agar-based assay
Avena sterilis
Petri-dish assay
whole-plant assay
Opis:
Different techniques have been devised to detect herbicide resistance in weeds, and the over- all aim from this study was to compare four different assay techniques for evaluating acetol- actate synthase (ALS)-inhibiting herbicide resistance in sterile wild oat (Avena sterilis L.). A resistant sterile wild oat population (R) was collected from the wheat field in Kozan, Adana province, Turkey. The susceptible (S) population was collected from the border of the same field. Effects of different doses of mesosulfuron-methyl + iodosulfuron-methyl- -sodium and pyroxsulam + cloquintocet-mexyl were assessed in agar based (seed and seed- ling) assay, Petri dish with seeds, and whole plant pot assay. In the agar based assays, the level of resistance was evaluated by measuring coleoptile and hypocotyl lengths, and sur- vival of seedlings. Plant height and shoot dry weight were measured in the Petri dish and whole plant pot assays, respectively. Results from the dose response analyses showed that both the R and S populations were extremely sensitive to mesosulfuron-methyl + iodosul- furon in the seedling bioassay. The resistance indices (RI’s) of the R biotype treated with mesosulfuron-methyl + iodosulfuron in the agar based seed, Petri dish, and whole plant assays were 2.29, 2.63 and 4.18, respectively. The resistance indices of the R biotype treated with pyroxsulam + cloquintocet-mexyl was 3.41, 5.05 and 2.82 in the agar based seed, Petri dish, and whole plant pot assays, respectively. The agar based seed assays and Petri dish as- say provided feasible, accurate, rapid, and cost effective opportunities to identify resistance in sterile wild oat.
Źródło:
Journal of Plant Protection Research; 2020, 60, 3; 244-252
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ultrasound conditioning of Streptococcus thermophilus CNRZ 447: growth, biofilm formation, exopolysaccharide production, and cell membrane permeability
Autorzy:
Khadem, H.
Tirtouil, A.M.
Drabo, M.S.
Boubakeur, B.
Powiązania:
https://bibliotekanauki.pl/articles/2096999.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Streptococcus thermophilus
ultrasound conditioning
bacterial adhesion
biofilm
exopolysaccharides
Opis:
Sonication is one of the new and innovative approaches that is being increasingly used in food industry to control fermentation processes and to eradicate spoiling. Recently, this approach has seen new industrial applications such as enhancing microbial productivity. The present study aimed to assess the effects of ultrasound conditioning on the metabolism and extracellular matrix production of Streptococcus thermophilus. Bacterial suspensions were treated in ultrasonic bath (35 kHz, 240/60 W peak/nominal power, 1.8 l capacity) for different time periods (5, 10, 15, 20, 30, 45, and 65 min), and the growth improvement, adhesion ability, biofilm formation, and exopolysaccharide production of the bacterial strain were measured. The bacterial strain exhibited resistance to the treatment, and the conditioning improved the growth, adhesion, membrane permeability, biofilm formation, and exopolysaccharide production ability. An optimal treatment was obtained for 30 minutes of conditioning. An excellent yield of desirable exopolysaccharides (1788 mg glucose equivalent/l) was achieved. Ultrasound conditioning may be used as a potential approach to enhance certain biotechnological properties of industrial microorganisms.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 159-165
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 34

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