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    Wyświetlanie 1-5 z 5
    Tytuł:
    Development of technology for obtaining water-soluble bacterial melanin and determination of some of pigment properties
    Autorzy:
    Aghajanyan, A.E.
    Vardanyan, A.A.
    Hovsepyan, A.C.
    Hambardzumyan, A.A.
    Filipenia, V.
    Saghyan, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/80261.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    melanin
    fermentation process
    aeration
    process optimization
    sorption
    dilution
    pigment property
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydrolysis of lactose from cheese whey using a reactor with [beta]-galactosidase enzyme immobilised on a commercial uf membrane
    Autorzy:
    Regenhardt, S.A.
    Mammarella, E.J.
    Rubiolo, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/184957.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    membrane bioreactor
    immobilised enzyme
    beta-galactosidas
    lactose hydrolysis
    bioreaktor membranowy
    enzym unieruchomiony
    beta-galaktozydazy
    hydroliza laktozy
    Opis:
    In this study, [Beta]-galactosidase enzyme from Kluyveromyces fragilis was immobilised on a commercial polyethersulfone membrane surface, 10 kDa cut-off. An integrated process, concerning the simultaneous hydrolysis–ultrafiltration of whey lactose was studied and working conditions have been fixed at 55[degrees]C and pH 6.9, the same conditions that are used for the industrial process of protein concentration. For the immobilisation, best results were obtained using 5% (v/v) of glutaraldehyde solution and 0.03 M galactose; the total activity recovery coefficient (TARC) was 44.2%. The amount of immobilised enzyme was 12.49 mg with a total activity of 86.3 LAU at 37[degrees]C, using 5% (w/v) lactose solution in phosphate buffer (100 mM pH 6.9). The stability of the immobilised enzyme was approximately 585 fold higher in comparison with the stability of free enzyme. Multipoint covalent immobilisation improves the stability of the enzyme, thereby enhancing the decision to use the membrane as a filtering element and support for the enzyme immobilisation.
    Źródło:
    Chemical and Process Engineering; 2013, 34, 3; 375-385
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The diagnostic importance of coagulation parameters in cattle having natural theileriosis
    Autorzy:
    Gunes, V.
    Onmaz, A.C.
    Keles, I.
    Varol, K.
    Ekinci, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/31055.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The purpose of this study was to determine the diagnostic importance of coagulation parameters in cattle with natural theileriosis. Nine Holstein cross-breed cattle with theileriosis as infected group and 6 healthy Holstein cattle as control group were used in the present study. Mean fibrinogen level, thrombin time (TT), activated partial thromboplastin time (aPTT) and prothrombin time (PT) were not statistically different when control and infected groups compared, except for the D-dimer concentration. Quantitative D-dimer concentrations were determined by immune-turbidimetric assay. D-dimer values increased significantly (p<0.05) in infected group (631.55 ± 74.41 μg/L) compared to control group (370.00 ± 59.94 μg/L). D-dimer sensitivity and specificity were also determined at cut-off concentrations (372 μg/L). Sensitivity and specificity of D-dimer values were determined to be 88.89% and 83.33%, respectively. D-dimer is thought to be important indicator in the evaluation of the prognosis in theileriosis cases. Analysis of D-dimer values before and after treatment in controlled case studies were suggested in future studies to enlighten the issue.
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 20, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
    Autorzy:
    Allegrucci, N.
    Velazquez, M.S.
    Russo, M.L.
    Perez, E.
    Scorsetti, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/65417.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    endophytic fungi
    tomato
    entomopathogen
    fungi
    Beauveria bassiana
    inoculation method
    tomato leafminer
    Tuta absoluta
    Lepidoptera
    Gelechiidae
    biological control
    Solanum lycopersicum
    Opis:
    Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interactions between the entomopathogenic fungus Beauveria bassiana and the Neotropical predator Eriopis connexa (Coleoptera: Coccinellidae): Implications in biological control of pest
    Autorzy:
    Scorsetti, A.C.
    Pelizza, S.
    Fogel, M.N.
    Vianna, F.
    Schneider, M.I.
    Powiązania:
    https://bibliotekanauki.pl/articles/65580.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    interaction
    entomopathogenic fungi
    Beauveria bassiana
    pathogen-predator interaction
    Eriopis connexa
    Coleoptera
    Coccinellidae
    biological control
    pest
    aphid
    Hemiptera
    Aphididae
    Opis:
    Aphids (Hemiptera: Aphididae) are serious pests of crops causing direct damage by feeding and indirect by the transmission of plant viruses. The use of conventional insecticides for controlling aphids has caused different problems and insecticide resistance. Accordingly, there is more interest in alternative control methods such as biological control by natural enemies for sustainable agricultural management. Among biological control agents, entomopathogenic fungi are one of the most significant microbial pathogens of insects. Also, Coccinellidae, as a major group, is a serious natural enemy. Both larval and adult stages of Coccinellidae feed on different soft-body pests, such as aphids. Eriopis connexa (Germar) (Coleoptera: Coccinellidae) is a common species in agroecosystems of the Neotropical region where it is considered to be a potential control agent. Pathogens and arthropod natural enemies may contribute to the control of phytophagous pests; however, it is important to assess potential interactions within biological control agents that share hosts (intraguild interaction) to evaluate their combined use for pest control. Therefore, the aim of this study was to evaluate the compatibility and interaction (lethal and sublethal effects) between E. connexa and the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales). Both are important biological control agents of aphids. The pathogenicity of B. bassiana against larvae, pupae and adults of the predator E. connexa was evaluated, and results showed, that B. bassiana infected the coleopteran. On the other hand, interaction between B. bassiana and the predator was evaluated through infected-prey. The effects of fungus on larvae survival were significantly different when we analyzed the accumulated survival (from first larval instar to adulthood). The daily fecundity was significantly reduced at five days compared to control group. By contrast, no significant differences were observed between the five oviposition days in the rate of hatched eggs. This study shows that despite having received a single dose of the fungus in its life cycle, the population parameters of the predator E. connexa are affected. More studies would be necessary to help identify interactions between microbes and natural enemies to increase and enhance opportunities and further develop biological pest control programs.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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