Wszystkie pola: kwiatek, M. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum aestivum L.)
Autorzy:: Kwiatek, M.
Pankiewicz, K
Wisniewska, H.
Korbas, M.
Danielewicz, J.
Powiązania:: https://bibliotekanauki.pl/articles/65147.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Endopeptidase marker EpD1b and STS marker XustSSR2001-7DL are closely linked to very effective eyespot resistance gene Pch1. Because of this, the aim of this study was to compare the results obtained under lab conditions using such markers with the results obtained under field conditions. 134 wheat breeding lines and Triticum aestivum L. var. Randevous used as a eyespot resistance control were analized. The combination of three methods allowed to select eight completely resistant or high resistant lines, that could be used in following breeding processes. Results obtained using endopeptidase and STS markers in 100% correlate with the phenotyping scoring.
Źródło:: Journal of Plant Protection Research; 2012, 52, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?
Autorzy:: Kwiatek, M.
Wisniewska, H.
Korbas, M.
Gawlowska, M.
Belter, J.
Majka, M.
Danielewicz, J.
Powiązania:: https://bibliotekanauki.pl/articles/66314.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: eyespot
resistance gene
wheat
winter wheat
breeding line
yield
inoculation
isoenzyme
molecular marker
plant resistance
Triticum aestivum
Opis:: Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Pch3 gene, originating from Dasypyrum villosum; and (4) the Q.Pch.jic-5A gene, a quantitative trait locus (QTL) located on chromosome 5A of Capelle Desprez. However, those loci have drawbacks, such as linkage of Pch1 with deleterious traits and limited effectiveness of Pch2 against the disease. Here we present an initial study which aims to characterize wheat pre-registration breeding lines carrying 12 eyespot resistance genes, consider their resistance expression in inoculation tests and the influence of resistance genotypes on the yield. We selected four groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A gene alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines. For the first time, the effect of the combination of Pch1 and Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.
Źródło:: Journal of Plant Protection Research; 2016, 56, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages
Autorzy:: Majka, M.
Kwiatek, M.
Korbas, M.
Danielewicz, J.
Gawlowska, M.
Goral, T.
Wisniewska, H.
Powiązania:: https://bibliotekanauki.pl/articles/65926.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: plant disease
fungal disease
eyespot
wheat
Triticum aestivum
resistance
winter wheat
plant breeding
breeding line
marker-assisted selection
inoculation
seedling
adult plant
Oculimacula yallundae
Oculimacula acuformis
Opis:: Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2 gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines: DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2 genes was identified, where the association between the Pch1 and the locus of the Xorw5 marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did not significantly affect the grain yield (from the plot), although the yield was highest in the presence of both genes. A significant effect of the presence of the Pch1 gene on thousand kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher TKW values than lines without both genes or with the Pch2 gene only.
Źródło:: Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Molecular diversity of Clostridium botulinum and phenotypically similar strains
Autorzy:: Grenda, T.
Kukier, E.
Sieradzki, Z.
Goldsztejn, M.
Kwiatek, K.
Powiązania:: https://bibliotekanauki.pl/articles/31310.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: This study was undertaken to examine phenotypic and genetic features of strains preliminary classified as Clostridium botulinum species. The phenotypic characteristics were assessed with different culture media and biochemical tests. The genetic characterization included detection of botulinum toxin genes by PCR and macrorestriction analysis with SmaI, XhoI and SacII by PFGE (Pulsed-field Gel Electrophoresis). Despite similar biochemical properties of all analysed strains, only 47% of them contained genes determining toxicity specific to C. botulinum species. The most valuable differentiation of C. botulinum and C. botulinum-like strains was obtained after SmaI digestion. The highest affinity was observed among C. botulinum type B profiles which was even up to 100%. It was found 100% of affinity between C. botulinum and C. botulinum-like strains, however, the similarity among C. botulinum and C. botulinum-like was generally lower than 80%.
Źródło:: Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues
Autorzy:: Sieradzki, Z.
Mazur, M.
Kwiatek, K.
Swiatkiewicz, S.
Swiatkiewicz, M.
Koreleski, J.
Hanczakowska, E.
Arczewska-Wlosek, A.
Goldsztejn, M.
Powiązania:: https://bibliotekanauki.pl/articles/31445.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: The aim of this study was to assess the possibility of genetically modified DNA transfer from feed containing RR soybean or/and MON810 maize to animal tissues, gut bacterial flora, food of animal origin, and the fate of GM DNA in the animal digestive tract. The experiment was carried out on broilers, laying hens, pigs and calves. All animals were divided into four groups: I – control group (non-modified feed), II – GM soybean group (non-modified maize, RR soybean), III – GM maize group (MON810 maize, non-modified soybean), and IV – GM maize and soybean group (MON810 maize, RR soybean). Samples of blood, organs, tissues, digesta from the gastrointestinal tract, and eggs were analysed for the presence of plant species specific genes, and transgenic sequences of CaMV 35S promoter and NOS terminator. PCR amplifications of these GM sequences were conducted to investigate the GM DNA transfer from feed to animal tissues and bacterial gut flora. In none of the analysed samples of blood, organs, tissues, eggs, excreta and bacterial DNA were plant reference genes or GM DNA found. A GM crop diet did not affect bacterial gut flora as regards diversity of bacteria species, quantity of particular bacteria species in the animal gut, or incorporation of transgenic DNA to the bacteria genome. It can be concluded that MON810 maize and RR soybean used for animal feeding are substantially equivalent to their conventional counterparts. Genetically modified DNA from MON810 maize and RR soybean is digested in the same way as plant DNA, with no probability of its transfer to animal tissues or gut bacterial flora.
Źródło:: Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Screening method for the determination of tetracyclines and fluoroquinolones in animal drinking water by liquid chromatography with diode array detector
Autorzy:: Patyra, E.
Kowalczyk, E.
Grelik, A.
Przenioslo-Siwczynska, M.
Kwiatek, K.
Powiązania:: https://bibliotekanauki.pl/articles/31196.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: A liquid chromatography - diode array detector (HPLC-DAD) procedure has been developed for the determination of oxytetracycline (OTC), tetracycline (TC), chlorotetracycline (CTC), doxycyc-line (DC), enrofloxacin (ENR), ciprofloxacin (CIP), sarafloxacin (SAR) and flumequine (FLU) residues in animal drinking water. This method was applied to animal drinking water. Solid-phase extraction (SPE) clean-up on an Oasis HLB cartridge allowed an extract suitable for liquid chromatographic analysis to be obtained. Chromatographic separation was carried out on a C18 analytical column, using gradient elution with 0.1% trifluoroacetic acid - acetonitrile - methanol at 30oC. The flow-rate was 0.7 mL/min and the eluate was analysed at 330 nm. The whole procedure was evaluated according to the requirements of the Commission Decision 2002/657/EC, determining specificity, decision limit (CCa), detection capacity (CCß), limit of detection (LOD), limit of quantification (LOQ), precision and accuracy during validation of the method. The recoveries of TCs and FQs from spiked samples at the levels of 10, 100 and 1000 ^g/L were higher than 82%. The developed method based on HPLC-DAD has been applied for the determination of four tetracyclines and four fluoroquinolones in animal drinking water samples.
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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