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Wyszukujesz frazę "fibroblast" wg kryterium: Wszystkie pola


Wyświetlanie 1-7 z 7
Tytuł:
Obtainment of transgenic porcine fibroblast cell lines for the purpose of xenotransplantation
Autorzy:
Hryhorowicz, M.
Nowak, A.
Grzeskowiak, B.
Zeyland, J.
Slomski, R.
Lipinski, D.
Powiązania:
https://bibliotekanauki.pl/articles/951232.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
pig
xenotransplantation
porcine fibroblast
cell line
interspecies somatic cell nuclear transfer
genetic modification
transgene expression
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos originating from transgenic foetal fibroblast cells
Autorzy:
Samiec, M.
Skrzyszowska, M.
Lipinski, D.
Powiązania:
https://bibliotekanauki.pl/articles/32261.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudophysiological activation
oocyte
reconstructed oocyte
nuclear-transferred embryo
pig
embryo
transgenic cell
fetal cell
fibroblast cell
preimplantation development
preimplantation development zob.tez embryonic development
cell cloning
Opis:
The completely new strategy of pseudophysiological transcomplementary (transcytoplasmic) activation (PP-TCA) of nuclear-transferred oocytes, which had been derived from pWAPhGH-GFPBsd transfected foetal fibroblast cells, was recently applied to the somatic cell cloning of pigs. It resulted in the considerable enhancing not only the cleavage activity of cultured cloned embryos, but also their morula and blastocyst formation rates as compared to the use of standard simultaneous fusion and electrical activation of reconstituted oocytes (77% vs. 57%, 63% vs. 46% and 40% vs. 27%, respectively). Altogether, the use of cytosolic components descended from heterologous (rabbit) zygotes as the agents for stimulation of porcine clonal cytoplasmic hybrids (cybrids) turned out to be reliable and feasible strategy for the generation of transgenic blastocysts by somatic cell nuclear transfer (SCNT). Furthermore, to our knowledge, no previous study has reported the preimplantation developmental outcome of transgenic nuclear-transferred pig embryos following the PP-TCA that was developed and optimised in our laboratory.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nichrome Capacitors on Polycarbonate Substrate for Monitoring Cell Culture Using Impedance Sensing Technique
Autorzy:
Kociubiński, A.
Zarzeczny, D.
Prendecka, M.
Pigoń, D.
Małecka-Massalska, T.
Powiązania:
https://bibliotekanauki.pl/articles/355589.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioimpedance
ecis
sputtering
nichrome
fibroblast
Opis:
The aim of this work was to present a method of tissue culture research by measuring the impedance of cells cultured in the presence of nichrome. For this purpose, the Electric Cell-substrate Impedance Sensing system was used with a prototype substrate containing comb capacitors made of nichrome. Magnetron sputtering, photolithography and etching processes were used to produce the thin-film electrodes. In the experimental part, cells of mouse fibroblast cell line L929 were cultured according to the instruction manual in complete medium, under controlled growth conditions. Inoculation of arrays was carried out by 300 microliters per well of cell suspension at ~1.2×105 cells/ml. The results of the monitoring cells behavior in tissue culture indicate good cell viability and proliferative potential.
Źródło:
Archives of Metallurgy and Materials; 2020, 65, 1; 493-496
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interactions between Marek’s disease virus Rispens/CVI988 vaccine strain and adenovirus field strain in chicken embryo fibroblast (CEF) cultures
Autorzy:
Nieczyporuk, J.S.
Wozniakowski, G.
Samorek-Salamonowicz, E.
Czekaj, H.
Powiązania:
https://bibliotekanauki.pl/articles/32188.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to determine the influence of adenovirus infection on the replication of Marek’s disease virus vaccine strain Rispens/CVI988 during in vitro co-infection studies. Adenovirus field strain JN-5/10j was isolated from sick chickens. The study was conducted in chicken embryo fibroblast cultures (CEF). Monolayers of CEFs were infected with Rispens strain and field adenovirus strain JN-5/10j with different doses (101.0-103.0 TCID₅₀) in the following manner: a) simultaneously, b) first, infection with Rispens strain and after 24 h infection with adenovirus strain JN-5/10j and c) infection with adenovirus strain JN-5/10j 24 h before infection with Rispens strain. After 18, 24, 48, 72, and 96 h of incubation, the copy number of the pp38 gene of Rispens strain was determined using Real-time PCR. The results indicated that the Adenovirus infection before the infection with Rispens strain reduced the replication of the pp38 gene after 48 h by 2 log₁₀.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative growth kinetic study of Newcastle disease virus, infectious bursal disease virus and avian influenza virus in chicken embryo fibroblast and DF-1 cell lines
Autorzy:
Anam, S.
Rahman, S.U.
Ali, S.
Saeed, M.
Goyal, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087170.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cytopathic effect
titer
growth kinetic
vaccine production
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 287-292
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and in vitro anticancer activity of N-alkyl phosphoramidate monoesters of 3N-azido-3N-deoxythymidine (AZT)
Autorzy:
Czajkowska-Wojciechowska, E.
Singh, A.
Trznadel, R.
Ruszkowski, P.
Celewicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/81295.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
anticancer activity
N-alkyl phosphoramidate monoester
3N-azido-3N-deoxythymidine
human cell line
cancer cell line
cervical cancer
HeLa cell line
KB cell
MCF-7 cell line
dermal fibroblast
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique
Autorzy:
Sucharski, P
Sanak, M.
Kostyk, E.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:
https://bibliotekanauki.pl/articles/2044221.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
COL1A1 gene
electrophoresis
COL1A2 gene
collagen production
man
RNA isolation
mutation
BESS-T-Scan technique
molecular diagnosis
DNA
osteogenesis imperfecta
cDNA synthesis
fibroblast culture
Opis:
A BESS-T-Scan analysis of cDNA COL1A1 and COL1A2 obtained by RT-PCR derived from five patients with sporadic forms of ostegenesis imperfecta was performed. The study was done in four patients with type I and one patient with type III OI. The analysis revealed the presence of structural changes in two regions of cDNA COL1A1 in two patients. No quantitative changes referring to COL1A2 gene were noted in any patient. The above analysis was the first application of the BESS-T-Scan technique in a molecular diagnosis of OI. The applied method seems to be useful and fulfil the basic criteria of the screening method to detect and locate mutations.
Źródło:
Journal of Applied Genetics; 1998, 39, 4; 367-373
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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