- Tytuł:
- Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test
- Autorzy:
-
Kaminski, S
Czarnik, U - Powiązania:
- https://bibliotekanauki.pl/articles/2046599.pdf
- Data publikacji:
- 1997
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection - Opis:
- In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.
- Źródło:
-
Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł