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Tytuł:
Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough
Autorzy:
Kalinowski, M.
Adaszek, L.
Miloszowska, P.
Skrzypczak, M.
Zietek-Barszcz, A.
Kutrzuba, J.
Gradzki, Z.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/31384.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular analysis
gene
canine adenovirus 2
isolation
dog
disease symptom
cough
upper respiratory tract infection
Opis:
The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on analysis of the obtained sequences, three polymorphic variants of CAV-2 (No.1-3) with homology of 78 - 100% were distinguished. The nucleotide and amino acid sequences of the most frequently represented polymorphic variant, No. 1, differed from the sequences of polymorphic variant No. 2 with one substitution. The nucleotide and amino acid sequence of the E1B 19K gene of CAV-2 AC 000003 differed from the analogous sequences of representatives of variant No. 1 with 44 nucleotide and 19 amino acid substitutions. The small number of nucleotide differences in the E1B 19K CAV-2 gene among the examined own isolates, compared with AC 000003, suggest that the infections in dogs were caused by a relatively genetically stable virus which occurs in eastern Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photoinduced Optical Properties Of Tl1-xIn1-xSixSe2 Single Crystals
Indukowane światłem właściwości optyczne monokryształów Tl1-xIn1-xSixSe2
Autorzy:
Myronchuk, G. L.
Zamurueva, O. V.
Oźga, K.
Szota, M.
El-Naggar, N. S.
Alzayed, N. S.
Piskach, L. V.
Parasyuk, O. V.
Albassam, A. A.
Fedorchuk, A. O.
Kityk, I. V.
Powiązania:
https://bibliotekanauki.pl/articles/352746.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Tl1-xIn1-xSixSe2 single crystal
chalcogenide crystals
optical properties
photoinduced absorption
dark electroconductivity
electroconductivity mechanisms
monokryształy Tl1-xIn1-xSixSe2
kryształy soli tlenowców
właściwości optyczne
absorpcja fotoindukowana
ciemna przewodność elektryczna
mechanizmy przewodności elektrycznej
Opis:
The influence of temperature on electroconductivity and photoinduced changes of the absorption at 0.15 eV under influence of the second harmonic generation of CO2 laser for the two type of single crystals were investigated. The single crystals Tl1−xIn1−xSixSe2 (x=0.1 and 0.2) have been grown by the two-zone Bridgaman-Stockbarger method. The temperature studies of electroconductivity were done in cryostat with thermoregulation in the temperature 77 - 300 K, with stabilization ±0.1 K. Photoinduced treatment of the investigated single crystals were performed using the 180 ns pulses second harmonic generation of the CO2 laser operating at 5.3 μm. Experimental studies have shown that for the Tl1−xIn1−xSixSe2 single crystals with decreasing temperature from 300 up to 240 K and from 315 up to 270 K the conductivity is realized by thermally excited impurities with activation energies equal to about 0.24 eV and 0.22 eV for x= 0.1 and 0.2, respectively. Photoinduced absorption achieves its maximum at a power density below 100 mJ/cm2. Has been shown that the samples with x=0.2 demonstrated higher changes of the photoinduced absorption with respect to the x=0.1. With further decreasing temperature is observed monotonic decrease in the activation energy of conductivity. The origin of these effects is caused by the excitations of both the electronic as well as phonon subsystem. At some power densities the anharmonic excitations become dominant and as a consequence the photoinduced absorption dependence is saturated what were observed. Additionally, we were evaluated at given temperature the average jump length of R for localized states near Fermi level.
W pracy badano wpływ temperatury na przewodnictwo elektryczne oraz indukowane światłem zmiany absorpcji optycznej przy 0.15 eV, pod działaniem drugiej harmonicznej lasera CO2 dla dwóch typów monokryształów. Monokryształy Tl1−xIn1−xSixSe2 (x=0.1 i 0.2) otrzymano w pionowym dwustrefowym piecu metodą Bridgamana-Stockbargera. Badania temperaturowe przewodności elektrycznej przeprowadzono w kriostacie z termoregulacją, w temperaturze 77-300 K, przy stabilizacji ±0,1 K. Fotoindukowaną obróbkę laserową monokryształów wykonano przy użyciu 180 ns impulsów drugiej harmonicznej lasera CO2 o długości fali 5,3 μm. Eksperymentalnie wykazano, że z obniżaniem temperatury od 300 do 240 K i od 315 do 270 K przewodnictwo elektryczne monokryształów Tl1−xIn1−xSixSe2 jest wywołane przez wzbudzenia termicznie domieszek z energią aktywacji równą około 0,24 eV i 0,22 eV dla ő = 0,1 i 0,2, odpowiednio. Indukowana optycznie absorpcja osiąga maksimum przy gęstości mocy poniżej 100 mJ/cm2. Stwierdzono, że próbka z x = 0,2 wykazuje większe zmiany absorpcji indukowanej światłem w porównaniu do próbki z x = 0.1. Z dalszym spadkiem temperatury obserwowano monotoniczny spadek energii aktywacji przewodnictwa. Pochodzenie tych efektów jest spowodowane przez wzbudzanie zarówno podsystemu elektronowego jak i fonononowego. Przy niektórych gęstościach mocy wzbudzenia anharmoniczne zaczynają dominować, co w konsekwencji prowadzi do nasycenia indukowanej światłem zależności absorpcji optycznej. Dodatkowo w pracy wyznaczono dla danej temperatury średnią długość skoku R dla stanów zlokalizowanych w pobliżu poziomu Fermiego.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 2A; 1051-1055
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of BHV-1 (bovine herpesvirus type 1) among non-vaccinated dairy cattle herds with respiratory disorders
Autorzy:
Rypula, K.
Ploneczka-Janeczko, K.
Kita, J.
Kumala, A.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/30913.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
seroprevalence
bovine herpesvirus type 1
non-vaccinated animal
dairy cattle
herd
respiratory disorder
ELISA test
seroconversion
Opis:
The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnological production of 1,3-propanediol from crude glycerol
Autorzy:
Drozdzynska, A.
Leja, K.
Czaczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/81121.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
1,3-propanediol
bioconversion
biotechnological production
chemical industry
crude oil
glycerol
Klebsiella pneumoniae
metabolic pathway
microorganism
natural fat
waste biomass
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of an atypical pigeon paramyxovirus type 1 in Poland
Autorzy:
Smietanka, K.
Minta, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31501.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
avian paramyxovirus
Polska
isolation
paramyxovirus infection
paramyxovirus type 1
pigeon
pigeon paramyxovirus type 1
poultry
Opis:
In this study, a pigeon paramyxovirus type 1 (PPMV-1) isolated from a flock of ornamental pigeons in Poland in 2010 is described. The PPMV-1/Poland/H2/10 isolate showed the amino acid sequence at the cleavage site of F2/F1 ¹¹²KRQKRF¹¹⁷ i.e. typical of virulent strains. Despite having the monoclonal antibody binding pattern typical of pigeon variants PPMV-1 (antigenic group “P”), the Polish isolate clustered into genetic sublineage 4a, which is usually associated with PMV-1 isolated from poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure-activity relationship study for fungicidal activity of 1-(4-phenoxymethyl-2-phenyl-[1,3]dioxolan-2-ylmethyl)-1H-1,2,4-triazole derivatives against rice blast
Autorzy:
Hoshi, T.
Yamada, K.
Yoshizawa, Y.
Oh, K.
Powiązania:
https://bibliotekanauki.pl/articles/66672.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antifungal agent
structure-activity relationship
fungicidal activity
fungicide
triazole derivative
rice
Oryza sativa
Magnaporthe oryzae
rice blast
plant disease
Opis:
To explore new antifungal agents for rice blast control, the antifungal activity of a series of novel 1,2,4-triazole derivatives against Magnaporthe oryzae has been evaluated. The antifungal activity was determined by using in vitro mycelial growth inhibition tests. Among the 19 test compounds, we found that the compound 1-(4-phenoxymethyl-2-phenyl-[1,3]dioxolan-2-ylmethyl)-1H-1,2,4- triazole (Gj) displayed potent antifungal activity against M. oryzae. The IC50 value was found approximately 3.8±0.5 μM and the IC50 value of propiconazole was found to be approximately 3.7±0.2 μM, respectively. Structure-activity relationship studies on aromatic ring structures provided insight and information about the structural requirements for antifungal activity of this synthetic series against M. oryzae.
Źródło:
Journal of Plant Protection Research; 2015, 55, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of real - time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses
Autorzy:
Stasiak, K.
Rola, J.
Zmudzinski, K.
Powiązania:
https://bibliotekanauki.pl/articles/32465.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0x100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real- time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Involvement of S1/P1-like nucleases in a plant programmed cell death
Autorzy:
Krela, R.
Lesniewicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/951226.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
programmed cell death
multicellular organism
morphogenesis
stress condition
nuclease
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic predisposition to breast and/or ovarian cancer – focus on the candidate BARD1 gene
Autorzy:
Klonowska, K.
Ratajska, M.
Wojciechowska, M.
Kozlowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/80894.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
breast cancer
ovarian cancer
genetic predisposition
BRCA1 gene
structure
function
BARD1 gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Raz jeszcze o obywatelstwie. W odpowiedzi na esej recenzyjny K.Gorlacha, M. Klekotko, P. Płucienniczaka: Obywatelstwo – społeczeństwo obywatelskie – państwo, „Studia Socjologiczne” 2013, 1 [208]
CITIZENSHIP REVISITED. REPLY TO REVIEW ESSAY BY K.GORLACH, M. KLEKOTKO AND P. PŁUCIENNICZAK: CITIZENSHIP – CIVIL SOCIETY – STATE, STUDIA SOCJOLOGICZNE 2013, 1 [208]
Autorzy:
Raciborski, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/428019.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Studia Socjologiczne; 2013, 2(209); 215-219
0039-3371
Pojawia się w:
Studia Socjologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Study Of The Die Roll Height Of SHP-1 And SCP-1 Materials In The Fine Blanking Process
Badanie wysokości deformacji krawędzi wycinanych elementów ze stali SHP-1 i SCP-1 procesie wykrawania
Autorzy:
Lee, C. K.
Kim, Y. C.
Powiązania:
https://bibliotekanauki.pl/articles/356499.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
die roll
fine blanking
SCP-1
share speed
SHP-1
v-rings
krawędzie
wykrawanie
szybkość działania
deformacja
Opis:
The height of the die roll, the distance of the V-ring, and the shear rate were varied with the aim of investigating the effects of the applied changes on the fine blanking line in a cold-rolled and a pickled steel sheet, referred to as SCP-1 and SHP-1, respectively. Both materials consisted primarily of a ferrite phase with small amounts of impurities including F, Mn, and Cr. The distance was found to be a very important factor in controlling the shear of the V-ring in the fine blanking process. When the position of the V-ring was set at distances of 1.5 mm and 2 mm, the die roll height increased with increasing shear speeds from 6.4 m/min to 10 and 16 m/min. Analysis of the influence of the shear rate revealed that low rates resulted in the lowest die roll heights since the flow of material was effectively inhibited.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 2B; 1397-1402
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Allelic variation at the VRN-1 locus of Polish cultivars of common wheat (Triticum aestivum L.)
Autorzy:
Nowak, M.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/19374.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
At a molecular level, the length of the vernalization period of common wheat (Triticum aestivum L.) is determined mainly by three loci: VRN-1, VRN-2 and VRN-3. In hexaploid wheat, the Vrn-A1, Vrn-B1 and Vrn-D1 genes are dominant for spring growth habit and epistatic to the alleles for winter growth habit. We used DNA markers to determine the VRN-1 genotypes of 43 common wheat cultivars from the Polish register. All of the 30 examined winter wheat cultivars carried the recessive vrn-A1 allele, and all of the 13 analyzed spring cultivars carried the dominant Vrn-A1a allele. Moreover, 13 winter and 11 spring cultivars carried the dominant Vrn-B1 allele. These results confirmed that the hexaploid wheat growth habit is determined mainly by the VRN-A1 locus.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2010, 52, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in Th1 and Th2 cytokine concentrations in ileal Peyer’s patches in gilts exposed to zearalenone
Autorzy:
Obremski, K.
Powiązania:
https://bibliotekanauki.pl/articles/32016.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
GALT induces tolerance to foreign food antigens and plays an important role in the development of food allergies and the inflammatory bowel disease. The immune function of GALT is significantly influenced by an equilibrium between Th1 and Th2 subpopulations and the cytokines they produce. Th1 cytokines participate in the induction of a cell-mediated immune response, whereas Th2 cytokines induce powerful antibody-mediated responses. Changes in Th1/Th2 cell polarization of an immune response are associated with susceptibility to autoimmune and infectious diseases. This experiment investigated changes in cytokine levels produced by Th1 and Th2 cells in ileal Payer’s patches in gilts exposed to ZEN doses below the NOEL (approximately 8 μg kg⁻¹ BW) for 14, 28 and 42 days. A significant linear increase in IL-4 (40.32 ± 1.55 ng mg⁻¹ - 137.60 ± 29.96 ng mg⁻¹), and IL-10 (5.99 ± 0.15 ng mg⁻¹ - 16.39 ± 1.11 ng mg⁻¹) concentrations was observed. An increase in Th1 (IL-2 and IFN-γ) cytokine levels was also noted in the experimental group, but it was not statistically significant. An HPLC analysis of Peyer’s patches in group E animals revealed a linear increase in ZEN concentrations (3.65 ± 0.91 ng g⁻¹ - 4.72 ± 1.85 ng g⁻¹) and an absence of α-ZEL. IL-4 stimulates monocytes and macrophages, it induces the production of proinflammatory cytokines and it may directly and indirectly contribute to the development of inflammatory foci. Higher IL-4 levels could shift polarization toward Th2 cells, stimulate B cells to undergo class switching to produce IgE and contribute to the development of allergies.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In silico prediction and characterization of three-dimensional structure of actin-1 of Arabidopsis thaliana
Autorzy:
Sahu, M.
Dehury, B.
Sarmah, R.
Sahoo, S.
Sahu, J.
Sarma, K.
Sen, P.
Modi, M.K.
Barooah, M.
Powiązania:
https://bibliotekanauki.pl/articles/80321.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
actin-1
protein sequence
Arabidopsis thaliana
comparative modelling
three-dimensional structure
molecular dynamics simulation
Opis:
Actin-1 is a ubiquitous protein belonging to the reproductive class of Actin family in Arabidopsis thaliana . This protein is involved in the formation of filaments that are major components of the cytoskeleton. Despite the importance of this protein, very little information is available regarding its structure and function in plants. In this study, analysis of the protein sequence was done and comparative model of Actin-1 was constructed (UNIPROT ID: P0CJ46) from Arabidopsis thaliana using the crystal structure of Dictyostelium discoideum actin (PDB ID: 1NLV-A) as template employing Modeller version 9.9. The stable structure was generated by 5 nanosecond molecular dynamics simulation steps using GROMOS43A1 96 force field that characterized its structural and dynamic feature. The biochemical function of the final simulated structure was also investigated using PROFUNC. The molecular simulation study suggested that the modeled Actin-1 protein retain its stable conformation in aqueous solution. The predicted binding sites in the modeled Actin-1 protein are very informative for further protein-ligand interaction study.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microstructure and Corrosion Behaviors of Biodegradable Mg-1Zn-1Zr-xSn Alloys Prepared by Powder-In-Tube Rolling
Autorzy:
Zakiyuddin, A.
Lee, K.
Powiązania:
https://bibliotekanauki.pl/articles/356604.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biodegradable Mg alloy
powder-in-tube rolling
corrosion behavior
Opis:
The objective of the present study was to investigate the effects of Sn addition on the mechanical and corrosion properties of Mg-1Zn-1Zr-xSn (x = 1, 2, 3, 4, 5 wt.%) alloys prepared by powder-in-tube rolling (PTR) method. The PTR-treated Mg alloys reached 98.3% of theoretical density. The hardness of the alloy increased with Sn addition. Two main intermetallic phases, Mg2Sn and Zn2Zr3, were formed in the alloys. The Mg2Sn intermetallic particles were observed along the grain boundaries, while the Zn2Zr3 particles were distributed in the Mg matrix. The addition of 1 wt. % Sn caused the corrosion potential to shift toward a more positive value, and the resulting alloy exhibited low corrosion current density.
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 3; 1467-1471
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
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