- Tytuł:
- LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots
- Autorzy:
-
Lei, X.
Ma, K.
Zhang, F. - Powiązania:
- https://bibliotekanauki.pl/articles/2117694.pdf
- Data publikacji:
- 2021
- Wydawca:
- Polska Akademia Nauk. Czasopisma i Monografie PAN
- Tematy:
-
cell cycle
Ca 2+ -channel blocker
RAPD
CRED-RA
Triticum aestivum - Opis:
- Accumulation of LaCl3 , a well-known Ca 2+ -channel blocker, can inhibit plant growth. However, the current understanding of its effects on gene expression is limited. In this paper, different concentrations of LaCl 3 (0, 0.5, 1.0, 1.5, 2.0 mM) were used to treat germinated wheat (Triticum aestivum L.) seeds for 24 h. The degree of root growth inhibition gradually increased with increasing LaCl 3 concentration. qRT-PCR analysis revealed that the expression of several key genes related to the cell cycle process, such as pcna, mcm2, rdr and cyclin B, were significantly down-regulated. Further analysis of genomic DNA instability using Random Amplified Polymorphic DNA (RAPD) and methylation levels by Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis indicated a significant increase in genomic DNA polymorphisms and methylation levels. The results of this study verified that the reasons why LaCl3 treatment can inhibit the growth of wheat roots are as follows: interference in the normal progression of the cell cycle, induction of genomic DNA instability and increase in DNA methylation levels.
- Źródło:
-
Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 1; 31-41
0001-5296 - Pojawia się w:
- Acta Biologica Cracoviensia. Series Botanica
- Dostawca treści:
- Biblioteka Nauki
Artykuł