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    Wyświetlanie 1-5 z 5
    Tytuł:
    First detection and molecular characterization of hepatitis E virus in water from wastewater treatment plants in Portugal
    Autorzy:
    Matos, A.
    Mesquito, J.R.
    Goncalves, D.
    Abreu-Silva, J.
    Luxo, C.
    Nascimento, M.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081721.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    wastewater
    hepatitis E virus
    genotype 3
    zoonotic
    Opis:
    Hepatitis E virus (HEV) genotype 3 is widespread in industrialized countries and widely recognized as a serious public health issue. HEV genotype 3 is excreted in both human and animal stools, having the potential to be introduced in aquatic environments through wastewater discharge. For this reason, waterborne transmission of the HEV genotype 3 is drawing increasing attention from the scientific community. Although studies in Portugal have shown that HEV circulates in both humans and swine, no study has yet focused on the presence of HEV in wastewater. Hence, a total of 60 water samples (influent and effluent) from 15 Wastewater Treatment Plants (WWTP) of different regions of Portugal were tested for the presence of HEV using a broad spectrum real-time RT-PCR with amplification within the ORF2 region of HEV genome. Positive samples were retested by nested RT-PCR with amplification within the ORF1 region, sequenced followed by phylogenetic analysis. HEV sequences were retrieved from two influent samples collected in December 2013 from WWTPs of North and Central of Portugal and classified in subgenotype 3i and 3f. This is the first study showing the presence of HEV in aquatic environments of Portugal.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 364-367
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
    Autorzy:
    Mravcova, K.
    Strkolcova, G.
    Mucha, R.
    Barbusinova, E.
    Goldova, M.
    Kacirova, J.
    Madar, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085903.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Slovakia
    nested PCR
    Cryptosporidium spp
    zoonotic subtype
    veterinary student
    Opis:
    Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 485-488
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of Chlamydophila psittaci antibodies from captive birds at the Ninoy Aquino Parks and Wildlife Nature Center, Quezon city, Philippines
    Autorzy:
    Maluping, R P
    Oronan, R.B.
    Toledo, S.U.
    Powiązania:
    https://bibliotekanauki.pl/articles/50103.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    parasitic bacteria
    Ninoy Aquino Park
    bird
    respiratory disease
    Quezon city
    Wildlife Nature Center
    Chlamydophila psittaci
    zoonotic disease
    Philippines
    Opis:
    The present study was undertaken to detect the presence of Chlamydophila psittaci antibodies in captive birds at the Wildlife Rescue Center, Ninoy Aquino Parks and Wildlife Nature Center, Quezon City, Philippines. Blood was collected from 36 birds of different species and the presence of antibodies against C. psittaci was detected using an ELISA-based test kit. 25% of the samples demonstrated antibodies against C. psittaci. The results of this study confi rmed the presence of C. psittaci antibodies among the captive birds examined.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Zwolinski, J.
    Zajac, V.
    Sawczuk, M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51615.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    polymerase chain reaction
    parasite
    genotyping
    Polska
    tick
    toxoplasmosis
    Ixodes ricinus
    B1 gene
    zoonotic disease
    occurrence
    Toxoplasma gondii
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs
    Autorzy:
    Ebani, V.V.
    Bertelloni, F.
    Turchi, B.
    Cerri, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/50930.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    serological survey
    molecular survey
    Anaplasma phagocytophilum
    hunting dog
    dog
    polymerase chain reaction
    indirect immunofluorescence antibody test
    tick
    zoonotic pathogen
    Italy
    prevalence
    Opis:
    The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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