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Wyświetlanie 1-4 z 4
Tytuł:
First detection and molecular characterization of hepatitis E virus in water from wastewater treatment plants in Portugal
Autorzy:
Matos, A.
Mesquito, J.R.
Goncalves, D.
Abreu-Silva, J.
Luxo, C.
Nascimento, M.S.
Powiązania:
https://bibliotekanauki.pl/articles/2081721.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
wastewater
hepatitis E virus
genotype 3
zoonotic
Opis:
Hepatitis E virus (HEV) genotype 3 is widespread in industrialized countries and widely recognized as a serious public health issue. HEV genotype 3 is excreted in both human and animal stools, having the potential to be introduced in aquatic environments through wastewater discharge. For this reason, waterborne transmission of the HEV genotype 3 is drawing increasing attention from the scientific community. Although studies in Portugal have shown that HEV circulates in both humans and swine, no study has yet focused on the presence of HEV in wastewater. Hence, a total of 60 water samples (influent and effluent) from 15 Wastewater Treatment Plants (WWTP) of different regions of Portugal were tested for the presence of HEV using a broad spectrum real-time RT-PCR with amplification within the ORF2 region of HEV genome. Positive samples were retested by nested RT-PCR with amplification within the ORF1 region, sequenced followed by phylogenetic analysis. HEV sequences were retrieved from two influent samples collected in December 2013 from WWTPs of North and Central of Portugal and classified in subgenotype 3i and 3f. This is the first study showing the presence of HEV in aquatic environments of Portugal.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 364-367
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ApoE genotype as risk factors for Alzheimer’s disease in the population of Lublin region, Poland
Autorzy:
Gustaw-Rothenberg, K.
Powiązania:
https://bibliotekanauki.pl/articles/3310.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
ApoE genotype
pathology
risk factor
human population
Lublin region
Polska
Alzheimer's disease
apolipoprotein
Źródło:
Journal of Pre-Clinical and Clinical Research; 2008, 02, 2
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of Acanthamoeba keratitis – a growing threat to human health worldwide
Autorzy:
Padzik, M.
Baltaza, W.
Conn, D.B.
Szaflik, J.P.
Chomicz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2081938.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
in vitro
Acanthamoeba Keratitis
A. polyphaga
T4 genotype
A. castellanii Neff
povidone iodine
chlorhexidine digluconate
toyocamycin effects
Opis:
Introduction. Free-living amoebae, ubiquitous in outer environments, in predisposing circumstances may exist as parasites, infectious agents of Acanthamoeba keratitis. In recent decades, the vision-threatening corneal infection is a growing human health threat worldwide, including Poland. The applied therapy is often ineffective due to diagnostic mistakes, various pathogenicity of Acanthamoeba strains and high resistance of cysts to drugs; many agents with possible anti-amoebic activity are still being tested. In the presented study, selected chemicals are investigated in terms of their in vitro effect on corneal and environmental Acanthamoeba strains. Materials and method. Samples of a corneal isolate from a patient with severe Acanthamoeba keratitis,of assessed on the basis of genotype associations of 18S rRNA and the type strain, Acanthamoeba castellanii Neff cultivated in bacteria-free condition, were exposed to povidone iodine, chlorhexidine digluconate or toyocamycin. In vitro population dynamics of the strains were monitored and compared to those of control cultures. Results. All chemicals showed anti-amoebic effects with different degrees of effectiveness. Significant differences were observed in the in vitro population dynamics, and the morpho-physiological status of A. castellanii Neff T4 and corneal strains determined as A. polyphaga T4 genotype, exposed to povidone iodine or toyocamycin, in comparison with chlorhexidine taken as reference. Conclusions. Time-dependent amoebstatic in vitro effects were demonstrated for all agents, in particular, the results of assays with povidone iodine are promising. No significant stimulation of encystation appeared; however, as cysticidal efficacy of chemicals is expected, complementary research is needed on different Acanthamoeba strains with modified agent concentrations and method application.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 725-731
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of Cryptosporidium and Giardia spp. in selected species of protected and game mammals from North-Eastern Poland
Autorzy:
Paziewska, A
Bednarska, M.
Nieweglowski, H.
Karbowiak, G.
Bajer, A.
Powiązania:
https://bibliotekanauki.pl/articles/51999.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
beaver
wolf
red deer
mammal
Giardia
Polska
reservoir
protozoan parasite
parasite
bison
man
genotype
parasitology
North-Eastern Poland
roe deer
Cryptosporidium
intestinal parasite
Opis:
Cryptosporidium spp. and Giardia spp. are wide-spread pathogens of humans and many species of mammals. The ways of transmission are very complex and diffi cult to defi ne. Both parasites occur in similar environments and share a broad host range. However, in Poland there is still little known about the epidemiology of these parasites due to the paucity of data on human cases and only few studies in wildlife. The aim of our study was to determine the distribution of two intestinal protozoa in a few species of protected and game mammals in North-Eastern Poland. Additionally, we wanted to compare prevalence and abundance of these parasites between wild and farm animals, and to determine the species/genotypes of Cryptosporidium. Fecal samples collected from protected species (European beaver – 22, grey wolf – 14, European bison – 55, Polish Konik (horse) – 5) and game mammals (red deer – 52, roe deer – 22, boar – 5) were examined by IFA. We also studied a group of samples collected from farm animals: beaver – 30, red deer – 66, Polish konik – 5. Cryptosporidium oocysts were identifi ed in 5 of 7 studied animal species (prevalence from 9% in roe deer to 36% in wolves), Giardia cysts in 4 of 6 studied species (prevalence from 1.7% in red deer to 7.7% in European beaver). Sequencing analysis of COWP gene fragment revealed that 5 Cryptosporidium isolates from wolves were C. parvum genotype 2 (zoonotic). The results show the important role of examined species in maintaining the natural sources of Cryptosporidium spp. and Giardia spp. infections in the environment.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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