Temat: Toxoplasma gondii - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Seroprevalence of Toxoplasma gondii and Neospora caninum in goats from north-western Spain
Autorzy:: Díaz, Pablo
Cabanelas, Eva
Díaz, José Manuel
Viña, Miguel
Béjar, Juan Pablo
Pérez-Creo, Ana
Prieto, Alberto
López, Ceferino Manuel
Panadero, Rosario
Fernández, Gonzalo
Díez-Baños, Pablo
Morrondo, Patrocinio
Powiązania:: https://bibliotekanauki.pl/articles/991154.pdf
Data publikacji:: 2016
Wydawca:: Instytut Medycyny Wsi
Tematy:: toxoplasma gondii
neospora caninum
goat
spain
risk factors
Opis:: Introduction and objective. Toxoplasma gondii and Neospora caninum are protozoans involved in reproductive failure especially in ruminant livestock. The objective was to estimate the seroprevalence of both parasites in goats from northwestern Spain and to study the influence of some factors on seropositivity. Materials and method. Blood samples from 638 goats were collected in 50 farms. Presence of T. gondii and N. caninum antibodies were detected by direct agglutination and competitiveELISA techniques, respectively. The risk factor analysis was performed using a mixed-effects logistic regression. Results. Individual (48%) and herd-level (74%) T. gondii seroprevalence values were high; the within-herd prevalence was 53%. In contrast, 6% of animals tested positive to N. caninum and 38% of the herds had at least one positive animal, with a true within-herd prevalence of 10%. Mixed infections were limited; 91% of N. caninum seropositive goats were also positive to T. gondii. The risk factor analysis showed that T. gondii seroprevalence is influenced by the presence of sheep in the farm (OR=4.9) and the seropositivity to N. caninum (OR=16.5); goats from the Central-coastal area, more humid and warm, had a 15.7-fold probability of being seropositive to T. gondii than those from the Mountainous area. Cross-breed goats (OR=4.5) and the seropositivity to T. gondii (OR= 9.5) were factors associated with N. caninum seropositivity. Conclusions. The high T. gondii seroprevalence in goats constitute a noticeable zoonotic risk. The consideration of the risk factors identified in designing T. gondii and N. caninum control programs in goat herds should allow the implementation of more efficient measures, avoiding the appearance of outbreaks of reproductive disorders by both protozoans in goats.
Źródło:: Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:: Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Powiązania:: https://bibliotekanauki.pl/articles/2081746.pdf
Data publikacji:: 2018
Wydawca:: Instytut Medycyny Wsi
Tematy:: Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy
Opis:: Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Źródło:: Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:: Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:: https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:: 2019
Wydawca:: Instytut Medycyny Wsi
Tematy:: Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:: Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:: Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Seroepidemiological study of canine Leishmania infantum and Toxoplasma gondii infections in Shanghai, China, and analysis of risk factors
Autorzy:: Jiang, Wei
Wang, Yan
Liu, Yingchun
Li, Tao
Chen, Yongjun
Wang, Shaohui
Han, Xiangan
Wang, Quan
Powiązania:: https://bibliotekanauki.pl/articles/990968.pdf
Data publikacji:: 2016
Wydawca:: Instytut Medycyny Wsi
Tematy:: seroprevalence
leishmania infantum
toxoplasma gondii
household dogs
risk factors
Opis:: The aim of this study was to determine the seroprevalence of Leishmania infantum and Toxoplasma gondii among household dogs in Shanghai (the most important industrial and commercial city in China), and to assess the possible risk factors associated with the infection. During 2014–2015, a total of 408 sera were collected from healthy household dogs and tested for L. infantum and T. gondii infection using commercial ELISA kits. The endemic characteristics according to gender, age group and breed were revealed by statistical descriptions and inference. The positive rates of L. infantum infection (24/408, 5.9%) were lower than those of T. gondii infection (37/408, 9.1%), and co-infection with both parasites was detected in seven dogs (7/408, 1.7%). Seropositivity for either parasite was more likely associated with age: the seroprevalence of T. gondii infection ranged from 1.3% (dogs≤1 year) – 18.7% (dogs>6 years), whereas that of L. infantum ranged from 1.3% (dogs≤1 year) – 9.9 % (dogs>6 years). Interestingly, the rates of exposure to both L. infantum and T. gondii were higher in males than in females. Relatively higher exposure rates for L. infantum and T. gondii were also observed in crossbred dogs compared with purebred dogs. However, neither gender nor breed is likely a determining factor for infection with these two parasites (P > 0.05). Identification of the risk factors that underlie these differences may help in the prevention of L. infantum and T. gondii infection in household dogs. To the best of our knowledge, this is the first report of L. infantum and T. gondii infection in household dogs in Shanghai, which shows that these two important parasites are still prevalent in this region. Therefore, it is necessary to take integrated strategies for prevention and control of infection in animals, which could help to reduce human infection in the region.
Źródło:: Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:: Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:: https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:: 2018
Wydawca:: Instytut Medycyny Wsi
Tematy:: flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:: Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:: Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
Autorzy:: Wójcik-Fatla, A.
Sroka, J.
Zając, V.
Sawczyn-Domańska, A.
Kloc, A.
Zwoliński, J.
Kłapeć, T.
Studzińska, M.B.
Chmura, R.
Dutkiewicz, J.
Powiązania:: https://bibliotekanauki.pl/articles/2085448.pdf
Data publikacji:: 2020
Wydawca:: Instytut Medycyny Wsi
Tematy:: environment
Coxiella burnetii
Toxoplasma gondii
veterinarians
Leptospira spp
Cryptosporidium spp
Giardia duodenalis
Opis:: Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
Źródło:: Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Potencjalna rola kleszczy z gatunków Dermacentor reticulatus i Ixodes ricinus w krążeniu pasożytniczych pierwotniaków: Theileria spp., Babesia spp. i Toxoplasma gondii w środowisku naturalnym
Potential role of ticks of the species Dermacentor reticulatus and Ixodes ricinus in the circulation of parasitic protozoa: Theileria spp., Babesia spp. and Toxoplasma gondii in the natural environment
Autorzy:: Wójcik-Fatla, Angelina
Kloc, Anna
Sawczyn, Anna
Zając, Violetta
Sroka, Jacek
Cisak, Ewa
Kulsharova, Aikerim
Dutkiewicz, Jacek
Powiązania:: https://bibliotekanauki.pl/articles/943335.pdf
Data publikacji:: 2016
Wydawca:: Instytut Medycyny Wsi
Tematy:: theileria spp.
babesia spp.
toxoplasma gondii
ixodes ricinus
dermacentor reticulatus
kleszcze
choroby odkleszczowe
Opis:: Wprowadzenie i cel pracy. Kleszcze są zaliczane do obligatoryjnych ektopasożytów kręgowców lądowych, w tym również i człowieka. Stanowią rezerwuar, niekiedy wektor wielu drobnoustrojów chorobotwórczych (bakterii, wirusów i pierwotniaków) zagrażających zdrowiu zwierząt i ludzi. Głównym celem pracy była próba oceny, czy powszechnie występujące w Polsce kleszcze z gatunku Ixodes ricinus i Dermacentor reticulatus mogą stanowić potencjalny wektor i rezerwuar dla pierwotniaków: Babesia i Theileria, oraz Toxoplasma gondii, a tym samym odgrywać rolę w krążeniu tych patogenów w przyrodzie. Skrócony opis stanu wiedzy. Do grupy pierwotniaków występujących w kleszczach należą rodzaje Theileria spp., Babesia spp., i jak stwierdzono w ostatnich badaniach – Toxoplasma gondii. Rodzaje Theileria i Babesia stanowią duże zagrożenie głównie dla zwierząt, powodując m.in. gorączkę wschodniego wybrzeża, tropikalną tejleriozę, babeszjozę. Z kolei Toxoplasma gondii to pierwotniak szczególnie niebezpieczny dla kobiet w ciąży, który może wywoływać trwałe i nieodwracalne wady płodu. Dotychczasowe badania potwierdzają obecność tych pierwotniaków u niektórych gatunków kleszczy, jak również możliwość współwystępowania niektórych z nich w jednym osobniku. Podsumowanie. Z punktu widzenia zdrowia publicznego, badania dotyczące potencjalnej roli kleszczy w transmisji pasożytniczych pierwotniaków mogą przyczynić się do oceny realnego zagrożenia nabycia infekcji w przypadku pokłucia przez kleszcze. Szczególne znaczenie dla kobiet ciężarnych miałyby wyniki dotyczące roli kleszczy jako alternatywnej drogi w transmisji Toxoplasma gondii – czynnika etiologicznego toksoplazmozy.
Introduction and objective. Ticks are classified into obligatory eco-parasites of terrestrial vertebrates, including humans. They constitute a reservoir, sometimes a vector of many pathological microorganisms (bacteria, viruses and protozoa) threatening the health of humans and animals. The primary objective of the study was an attempt to evaluate whether ticks of the species Ixodes ricinus and Dermacentor reticulatus, commonly occurring in Poland, may constitute a potential vector and reservoir for the protozoa: Babesia, Theileria, and Toxoplasma gondii, and at the same time, play a role in the circulation of these pathogens in nature. Brief description of the state of knowledge. The genera Theileria spp., Babesia spp, and as has been found in recent years, Toxoplasma gondii belong to the group of protozoa occurring in ticks. The genera Theileria and Babesia create a great risk, mainly for animals, causing, among others, east coast fever, tropical theileriosis and babesiosis. In the case of Toxoplasma gondii, this protozoan is especially dangerous for pregnant women, and may cause permanent and irreversible foetal defects. Studies to-date confirm the presence of these protozoa in some species of ticks, as well as the possibility of co-occurrence of some of them in one individual tick. Summing up. From the aspect of public health, studies concerning the potential role of ticks in the transmission of parasitic protozoa may contribute to the assessment of the actual risk of infection in the case of tick bites. The results concerning the role of ticks as an alternative route of transmission of T. gondii – the etiologic factor of toxoplasmosis, would be of a special importance for pregnant women.
Źródło:: Medycyna Ogólna i Nauki o Zdrowiu; 2016, 22(51), 3
2083-4543
Pojawia się w:: Medycyna Ogólna i Nauki o Zdrowiu
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Preliminary assessment of usefulness of cELISA test for screening pig and cattle populations for presence of antibodies against Toxoplasma gondii
Autorzy:: Sroka, J.
Karamon, J.
Cencek, T.
Dutkiewicz, J.
Powiązania:: https://bibliotekanauki.pl/articles/50742.pdf
Data publikacji:: 2011
Wydawca:: Instytut Medycyny Wsi
Tematy:: usefulness
cELISA test
screening
pig
cattle
animal population
serology
antibody
Toxoplasma gondii
toxoplasmosis
Źródło:: Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Is congenital toxoplasmosis still an important clinical problem?
Autorzy:: Alghamdi, Ali Joman
Alabbas, Ali
Tadla, Monika
Agyemfra-Kumi, Catherine
Tarkowska, Agata
Furmaga-Jabłońska, Wanda
Powiązania:: https://bibliotekanauki.pl/articles/972434.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: congenital toxoplasmosis
risk factors
prophylaxy
undercooked meat
Toxoplasma gondii
Good hygiene
Spiromycin
Opis:: Introduction. Congenital toxoplasmosis is one of the most common intrauterine inherited diseases. The risk and severity of infection depends on the time of transmission during pregnancy. The earlier the infection, the lower the risk of transmission but with serious estimated outcomes and vice versa. Case report. A newborn with congenital toxoplasmosis was born to a mother who was seronegative at the beginning of pregnancy, and was not aware of toxoplasmosis risk factors during pregnancy. The newborn was born with features of small for gestational age and with intracranial calcification revealed in cranial ultrasonography, which emphasized the need for further investigations. Neonatal serological panel and cerebrospinal fluid analysis were both confirmative of congenital toxoplasmosis. Ophthalmological examination showed the typical whitish focus of retinochoroiditis which later led to a blindness in one eye. Additionally, hydrocephalus was progressing slowly until it became stable in 5 months. Objective. The aim of this study was to present the case of congenital toxoplasmosis in order to underline the lack of social awareness concerning risk factors in pregnant women. Thus, educational campaigns against the consumption of raw meat and unpasteurized milk while pregnant may prevent the recurrence of such cases. Moreover, proper washing of fruits and vegetables are advised in this respect.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2017, 11, 2; 167-170
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Seroprevalence of Toxoplasma gondii in wild boars [Sus scrofa] in the Slovak Republic
Autorzy:: Antolova, D
Reiterova, K.
Dubinsky, P.
Powiązania:: https://bibliotekanauki.pl/articles/49413.pdf
Data publikacji:: 2007
Wydawca:: Instytut Medycyny Wsi
Tematy:: parasite
toxoplasmosis
wild boar
Toxoplasma gondii
Sus scrofa
Slovak Republic
seroprevalence
protozoan parasite
Opis:: Toxoplasma gondii is a protozoan parasite of great medical and veterinary importance. The aim of this study was to determine the seroprevalence of toxoplasmosis in wild boars hunted in the Slovak Republic in 2003. Examination of 320 wild boars revealed a seroprevalence of 8.1%. The majority of seropositive wild boars came from the north-western and southern regions of Slovakia. This study indicates that T. gondii infection is common in wild boars in the Slovak Republic, underlines its zoonotic potential and the importance of high standards of hygiene during the handling of game.
Źródło:: Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Environmental exposure of pregnant women to infection with Toxoplasma gondii - state of the art
Autorzy:: Bojar, I
Szymanska, J.
Powiązania:: https://bibliotekanauki.pl/articles/49419.pdf
Data publikacji:: 2010
Wydawca:: Instytut Medycyny Wsi
Tematy:: environmental exposure
woman
pregnant woman
woman exposure
human disease
toxoplasmosis
Toxoplasma gondii
infection
environmental factor
Źródło:: Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 209-214
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Prevalence of infections and co-infections with 6 pathogens in Dermacentor reticulatus ticks collected in eastern Poland
Autorzy:: Zając, Violetta
Wójcik-Fatla, Angelina
Sawczyn, Anna
Cisak, Ewa
Sroka, Jacek
Kloc, Anna
Zając, Zbigniew
Buczek, Alicja
Dutkiewicz, Jacek
Bartosik, Katarzyna
Powiązania:: https://bibliotekanauki.pl/articles/989947.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: dermacentor reticulatus
co-infections
tbe virus
anaplasma phagocytophilum
rickettsia raoultii
borrelia burgdorferi sensu
lato
babesia spp.
toxoplasma gondii
Opis:: Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species. An attempt was made to determine the significance of the associations between 6 different pathogens occurring in D. reticulatus (Tick-borne encephalitis virus = TBEV, Anaplasma phagocytophilum, Rickettsia raoultii, Borrelia burgdorferi s. l., Babesia spp., Toxoplasma gondii), using 2 statistical methods: determination of Odds Ratios (ORs) and the Fisher’s exact test. 634 questing Dermacentor reticulatus ticks (370 females and 264 males) were collected in 2011–2013 by flagging the lower vegetation in 3 localities in the area of Łęczyńsko-Włodawskie Lakeland, situated in the Lublin region of eastern Poland. The presence of individual pathogens was detected by PCR. Ticks were infected most often with Rickettsia raoultii (43.8%), less with TBEV (8.5%), and much less with Babesia spp., Toxoplasma gondii, Borrelia burgdorferi s.l., and Anaplasma phagocytophilum (2.5%, 2.1%, 1.6% and 1.1%, respectively). The locality-dependent variability proved to be significant for TBEV (c2=11.063; P=0.004) and Toxoplasma gondii (c2=11.298; P=0.0035), but not for other pathogens. Two hundred seventy (42.6%) of the examined ticks were infected only with a single pathogen, and 54 (8.5%) showed the presence of dual co-infections, each with 2 pathogens. The most common were dual infections with participation of Rickettsia raoultii (7.41%); next, those with participation of the TBEV (5.21%), Toxoplasma gondii (1.58%), Borrelia burgdorferi s.l. (1.26%), Anaplasma phagocytophilum (0.95%), and Babesia spp. (0.63%). On the total number of 15 possible associations, in 9 cases co-infections occurred whereas in 6 cases they were not detected. The most noteworthy were positive co-infections with the participation of TBEV, which proved to be weakly significant (0.05
Źródło:: Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki