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    Wyświetlanie 1-11 z 11
    Tytuł:
    Mechanizmy infekcji patogenami przenoszonymi przez kleszcze na przykładzie bakterii: Anaplasma phagocytophilum i Borrelia burgdorferi
    Mechanisms of infection by pathogens transmitted by ticks on the example of bacteria: Anaplasma phagocytophilum and Borrelia burgdorferi
    Autorzy:
    Wróblewska, Paula
    Adamczuk, Piotr
    Galińska, Elżbieta Monika
    Chmielewska-Badora, Jolanta
    Zwoliński, Jacek
    Chmielewski, Jarosław
    Powiązania:
    https://bibliotekanauki.pl/articles/1035574.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    "Anaplasma phagocytophilum"
    "Borrelia burgdorferi"
    "kleszcze"
    "mechanizmy infekcji"
    Opis:
    Tick-borne diseases are transmission diseases belonging to the group of zoonoses but carried by ticks. These diseases are a major public health problem but also a problem for groups occupationally exposed to tick bites. Ixodes ricinus is a species of ticks which is the most common reservoir and the vector of a large number of microorganisms pathogenic to humans. It transfers, among others, bacteria of the species: Anaplasma phagocytophilum and Borrelia burgdorferi. The article discusses the mechanisms of infection with Borrelia burgdorferi and Anaplasma phagocytophilum for both ticks as well as for animals and humans. The two microorganisms discussed have developed many characteristics and mechanisms of adaptation to the environment, as well as defense mechanisms against the body's immune response. Understanding the biology of ticks and the function of proteins produced by ticks and pathogenic microorganisms is the key in the development of effective treatments and prevention of Lyme disease and anaplasmosis.
    Choroby odkleszczowe są chorobami transmisyjnymi, należącymi do grupy chorób odzwierzęcych, ale przenoszonych za pośrednictwem kleszczy. Choroby te stanowią ważny problem zarówno zdrowia publicznego, ale także problem dla grup zawodowo narażonych na ukłucia kleszczy. Ixodes ricinus jest gatunkiem kleszcza, który jest najczęstszym rezerwuarem i wektorem licznych mikroorganizmów wywołujących choroby ludzi. Przenosi on między innymi bakterie z gatunków: Anaplasma phagocytophilum i Borrelia burgdorferi. W artykule zostały omówione mechanizmy infekcji Borrelia burgdorferi i Anaplasma phagocytophilum zarówno kleszcza, ale także zwierząt i ludzi. Obydwa omawiane mikroorganizmy wykształciły wiele cech i mechanizmów przystosowawczych do środowiska, a także mechanizmów obronnych przed odpowiedzią immunologiczną organizmu. Poznanie biologii kleszczy, funkcji białek wytwarzanych przez kleszcze i patogenne mikroorganizmy stanowi klucz w opracowaniu skutecznych metod leczenia i profilaktyki boreliozy i anaplazmozy.
    Źródło:
    Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 2; 63-68
    1505-7054
    2084-6312
    Pojawia się w:
    Medycyna Środowiskowa - Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First case of human granulocytic anaplasmosis from Slovakia
    Autorzy:
    Novakova, M
    Vichova, B.
    Majlathova, V.
    Lesnakova, A.
    Pochybova, M.
    Pet'ko, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/50355.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    anaplasmosis
    man
    granulocytic anaplasmosis
    Anaplasma phagocytophilum
    Ixodes ricinus
    tick-borne disease
    Slovakia
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 173-175
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion
    Autorzy:
    Wojcik-Fatla, A
    Szymanska, J.
    Wdowiak, L.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51180.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick
    Ixodes ricinus
    coincidence
    pathogen
    Borrelia burgdorferi
    Anaplasma phagocytophilum
    Babesia microti
    Lublin macroregion
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 151-158
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological evidence of exposure to zoonotic tick-borne bacteria in pheasants (Phasianus colchicus)
    Autorzy:
    Ebani, Valentina Virginia
    Bertelloni, Fabrizio
    Mani, Paolo
    Powiązania:
    https://bibliotekanauki.pl/articles/988938.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    pheasant (phasianus colchicus)
    anaplasma phagocytophilum
    borrelia burgdorferi s.l.
    coxiella burnetii
    sfg rickettsia spp.
    ifat
    Opis:
    Introduction and objective. Previous studies on tick-borne pathogens in the avian population have focused mainly on the detection of the agents in ticks collected from birds, but data about the presence of tick-borne bacteria in these animals are scant. The aim of the presented study was to verify the exposure to some zoonotic tick-borne bacteria, in particular, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Coxiella burnetii, and the Rickettsia species of the Spotted Fever Group (SFG), in pheasants (Phasianus colchicus) living in a central Italy area, characterized by conditions favourable for the diffusion of the ticks. Materials and method. Blood serum samples from 276 farm-reared pheasants were examined by indirect immunofluorescence antibody test to detect antibodies against the 4 reported pathogens. Results. A total of 124 (44.92%) birds resulted as seropositive: in particular 3 (1.08%) to C. burnetii, 31 (11.23%) to A. phagocytophilum, 46 (16.67%) to B. burgdorferi s.l., 49 (17.75%) to SFG Rickettsia spp. antigens. Three pheasants resulted positive both to A. phagocytophilum and B. burgdorferi s.l. antigens, whereas 2 – both to B. burgdorferi s.l. and SFG Rickettsia spp. antigens. The antibody titers varied from 1:40–1:320. Conclusions. The obtained results show that the pheasants had developed antibodies to the investigated tick-borne agents. For this reason, they seem to be involved in the epidemiology of the studied tick-borne bacteria.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of infections and co-infections with 6 pathogens in Dermacentor reticulatus ticks collected in eastern Poland
    Autorzy:
    Zając, Violetta
    Wójcik-Fatla, Angelina
    Sawczyn, Anna
    Cisak, Ewa
    Sroka, Jacek
    Kloc, Anna
    Zając, Zbigniew
    Buczek, Alicja
    Dutkiewicz, Jacek
    Bartosik, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/989947.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    dermacentor reticulatus
    co-infections
    tbe virus
    anaplasma phagocytophilum
    rickettsia raoultii
    borrelia burgdorferi sensu
    lato
    babesia spp.
    toxoplasma gondii
    Opis:
    Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species. An attempt was made to determine the significance of the associations between 6 different pathogens occurring in D. reticulatus (Tick-borne encephalitis virus = TBEV, Anaplasma phagocytophilum, Rickettsia raoultii, Borrelia burgdorferi s. l., Babesia spp., Toxoplasma gondii), using 2 statistical methods: determination of Odds Ratios (ORs) and the Fisher’s exact test. 634 questing Dermacentor reticulatus ticks (370 females and 264 males) were collected in 2011–2013 by flagging the lower vegetation in 3 localities in the area of Łęczyńsko-Włodawskie Lakeland, situated in the Lublin region of eastern Poland. The presence of individual pathogens was detected by PCR. Ticks were infected most often with Rickettsia raoultii (43.8%), less with TBEV (8.5%), and much less with Babesia spp., Toxoplasma gondii, Borrelia burgdorferi s.l., and Anaplasma phagocytophilum (2.5%, 2.1%, 1.6% and 1.1%, respectively). The locality-dependent variability proved to be significant for TBEV (c2=11.063; P=0.004) and Toxoplasma gondii (c2=11.298; P=0.0035), but not for other pathogens. Two hundred seventy (42.6%) of the examined ticks were infected only with a single pathogen, and 54 (8.5%) showed the presence of dual co-infections, each with 2 pathogens. The most common were dual infections with participation of Rickettsia raoultii (7.41%); next, those with participation of the TBEV (5.21%), Toxoplasma gondii (1.58%), Borrelia burgdorferi s.l. (1.26%), Anaplasma phagocytophilum (0.95%), and Babesia spp. (0.63%). On the total number of 15 possible associations, in 9 cases co-infections occurred whereas in 6 cases they were not detected. The most noteworthy were positive co-infections with the participation of TBEV, which proved to be weakly significant (0.05
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland
    Autorzy:
    Asman, M.
    Witecka, J.
    Solarz, K.
    Zwonik, A.
    Szilman, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085175.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Ixodes ricinus
    Opolskie Province
    Polska
    Borrelia burgdorferi sensu lato
    Anaplasma phagocytophilum
    Babesia microti
    Borrelia burgdorferi sensu lato genospecies
    Opis:
    Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis. Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland. Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively. Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 544-547
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Co-infection with Borrelia species and other tick-borne pathogens in humans: two cases from Poland
    Autorzy:
    Welc-Faleciak, R
    Hildebrandt, A.
    Sinski, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/49402.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human disease
    human health
    tick-borne disease
    tick-borne pathogen
    man
    Borrelia
    Anaplasma phagocytophilum
    Babesia
    co-infection
    Polska
    prevalence
    Ixodes ricinus
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 309-313
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs
    Autorzy:
    Ebani, V.V.
    Bertelloni, F.
    Turchi, B.
    Cerri, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/50930.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    serological survey
    molecular survey
    Anaplasma phagocytophilum
    hunting dog
    dog
    polymerase chain reaction
    indirect immunofluorescence antibody test
    tick
    zoonotic pathogen
    Italy
    prevalence
    Opis:
    The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anaplasma phagocytophilum infection of red foxes [Vulpes vulpes]
    Autorzy:
    Karbowiak, G
    Vichova, B.
    Majlathova, V.
    Hapunik, J.
    Pet'ko, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/50073.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Mazovia region
    parasite
    Anaplasma phagocytophilum
    infection
    animal disease
    red fox
    Vulpes vulpes
    16S rRNA gene
    msp4 gene
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 299-300
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of IgG antibodies to Anaplasma phagocytophilum in humans suspected of Lyme borreliosis in Eastern Slovakia
    Autorzy:
    Kalinova, Z
    Halanova, M.
    Cislakova, L.
    Sulinova, Z.
    Jarcuska, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/51005.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human disease
    tick-borne disease
    human granulocytic anaplasmosis
    zoonosis
    Anaplasma phagocytophilum
    Ixodes ricinus
    Lyme borreliosis
    Slovakia
    antibody
    occurrence
    Borrelia burgdorferi
    IgG antibody
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 285-288
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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