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    Wyświetlanie 1-7 z 7
    Tytuł:
    Oxidative stress assessment in lung of lead-exposed rats
    Ocena stresu oksydacyjnego w płucach szczurów narażonych na ołów
    Autorzy:
    Machoń-Grecka, Anna
    Kaletka, Zbigniew
    Pryzwan, Tomasz
    Szczęsny, Patrycja
    Maciejczyk, Maciej
    Kasperczyk, Aleksandra
    Dobrakowski, Michał
    Powiązania:
    https://bibliotekanauki.pl/articles/1035320.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    "lead poisoning"
    "lung"
    "oxidative stress"
    Opis:
    Objective. Little is known about lead toxic effects on lung tissue. Therefore, the present study was undertaken to estimate the values of selected parameters related to oxidative stress in the lung of rats exposed to lead acetate. Material and methods. Twenty male Wistar rats were randomly divided into two groups: control (n=10) and lead-treated (n=10). The lead-treated group was fed with regular rat chow and distilled water supplemented with lead acetate (1200 ppm) for 7 days. In lung homogenates, the level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD, CuZn-SOD, Mn-SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined. Results. The activity of catalase was significantly higher in the lung of lead-treated rats than in controls by 98%. Similarly, the activities of GPx and GR were higher by 57% and 45%, respectively. The level of MDA was also higher in lead-exposed rats than in the control group by 52%. The remaining parameters did not differ between the studied groups. Conclusions. Lead exposure stimulates antioxidant defense mechanisms in rat lung; however, these mechanisms are insufficient to prevent lead-induced oxidative stress development
    Wstęp. Toksyczny wpływ ołowiu na tkankę płucną jest słabo poznany. W związku z tym, prezentowany eksperyment miał na celu ocenę zmian w wybranych parametrach związanych ze stresem oksydacyjnym w tkance płucnej u szczurów narażonych na octan ołowiu. Materiał i metody. Dwadzieścia szczurów szczepu Wistar, podzielono na dwie grupy: kontrolną (n=10) i badaną z podawanym ołowiem (n=10). Szczury z grupy badanej były regularnie karmione granulatem dla szczurów i pojone wodą destylowaną z octanem ołowiu (1200 ppm) przez 7 dni. W homogenatach z tanki płucnej oznaczany był poziom malonylodialdehydu (MDA) i aktywność dysmutazy ponadtlenkowej (SOD, CuZn-SOD, Mn-SOD), katalazy (CAT), peroksydazy glutationowej (GPx), reduktazy glutationowej (GR), oraz transferazy glutationowej (GST). Wyniki. Aktywność katalazy była o 98% wyższa w grupie badanej aniżeli w grupie kontrolnej. Podobnie, aktywności GPx i GR były wyższe odpowiednio o 57% i 45%. Poziom MDA był również wyższy w grupie badanej, o 52% w porównaniu do grupy kontrolnej. Pozostałe parametry nie różniły się pomiędzy grupami. Wnioski. Ekspozycja na ołów stymuluje mechanizmy obrony antyoksydacyjnej, jednakże mechanizmy te są niewystarczające by zapobiec stresowi oksydacyjnemu wywołanemu działaniem ołowiu.
    Źródło:
    Medycyna Środowiskowa - Environmental Medicine; 2018, 21, 1; 21-24
    1505-7054
    2084-6312
    Pojawia się w:
    Medycyna Środowiskowa - Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of citrus flavanones on the redox homeostasis in cells exposed to oxidative stress – studies in vitro
    Wpływ flawanonów cytrusowych na homeostazę redoks komórek narażonych na stres oksydacyjny – badania in vitro
    Autorzy:
    Kurzeja, Ewa
    Synowiec-Wojtarowicz, Agnieszka
    Spiołek, Katarzyna
    Derewniuk, Małgorzata
    Pawłowska-Góral, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1035565.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    "cell culture"
    "flavanones"
    "oxidative stress"
    "redox homeostasis"
    Opis:
    Introduction: Flavanones (an important group of antioxidants in citrus fruits) are beneficial for health, which is connected with their anti-inflammatory, anti-atherogenic and anti-carcinogenic properties. The present study was undertaken to investigate whether – and in what way – the presence of flavanones influences the redox homeostasis of fibroblasts and alleviates the effects of oxidative stress. Material and methods: The study was conducted on murine fibroblast cell cultures with the addition of flavanones (hesperidin, hesperetin, naringin, naringenin), exposed to oxidative stress (Fe/Asc). In cell homogenates, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) was measured; in the medium, the concentration of nitric oxide was measured. Results and conclusion: Our results demonstrate that the addition of naringenin, hesperetin, naringin and hesperidin has a protective effect on cells subjected to oxidative stress The changes observed are particularly visible in the case of aglycone forms of both compounds. Despite the protective properties against oxidative stress which flavanones display, we determined distrubances in redox homeostasis in comparison to the control culture.
    Wstęp: Flawanony, będące głównymi związkami bioaktywnymi cytrusów, wykazują działanie prozdrowotne, co wynika z ich właściwości przeciwzapalnych, przeciwmiażdżycowych i przeciwnowotworowych. Celem badania była ocena wpływu flawanonów na homeostazę redoks fibroblastów oraz czy ich obecność łagodzi skutki stresu oksydacyjnego. Materiały i metody: Badanie przeprowadzono na hodowlach mysich fibroblastów z dodatkiem flawanonów (hesperydyna, hesperetyna, naringina, naringenina) poddanych stresowi oksydacyjnemu (Fe/Asc). W homogenatach komórkowych oznaczono aktywność dysmutazy ponadtlenkowej (SOD) i peroksydazy glutationowej (GPx), a bezpośrednio w pożywce stężenie tlenku azotu. Wyniki i wnioski: Wyniki naszej pracy wskazują, że dodatek naringeniny, hesperetyny, naringiny i hesperydyny działa ochronnie na komórki poddane stresowi oksydacyjnemu. Pomimo stwierdzonego ochronnego działania flawanonów zaobserwowaliśmy również zaburzenie homeostazy redoks w odniesieniu do hodowli kontrolnej.
    Źródło:
    Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 2; 32-39
    1505-7054
    2084-6312
    Pojawia się w:
    Medycyna Środowiskowa - Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Glutathione level and glutathione reductase activity in serum of coronary heart disease patients
    Autorzy:
    Zuzak, Ewa
    Horecka, Anna
    Kiełczykowska, Małgorzata
    Dudek, Aneta
    Musik, Irena
    Kurzepa, Joanna
    Kurzepa, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/972495.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    glutathione reductase
    glutathione
    coronary heart disease
    oxidative stress
    Opis:
    Introduction. Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD. Materials and method. Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coronary catheterization procedure (in study patients). GR activities and GSH concentration was determined using commercially available colorimetric kits. Results. The highest GR activity and GSH serum level was noticed in UA patients. However, statistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no significant differences in GSH between the CHD patients and controls. Conclusion. The elevated activity of glutathione reductase in serum of patients with unstable angina pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syndromes.
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2017, 11, 2; 103-105
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protective effects of molecular hydrogen on hepatotoxicity induced by sub-chronic exposure to chlorpyrifos in rats
    Autorzy:
    Xun, ZM.
    Xie, F.
    Zhao, PX.
    Liu, MY.
    Li, ZY.
    Song, JM.
    Kong, XM.
    Ma, XM.
    Li, XY.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085965.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    oxidative stress
    hepatotoxicity
    chlorpyrifos
    molecular hydrogen
    mitochondrial pathway
    Opis:
    Introduction. Chlorpyrifos (CPF) is a organophosphate insecticide widely used in agriculture with attendant adverse health outcomes. Chronic exposure to CPF induces oxidative stress and elicits harmful effects, including hepatic dysfunction. Molecular hydrogen has been identified as a novel antioxidant which could selectively scavenge hydroxyl radicals. Objective. The aim of this study was to determine whether the intake of hydrogen-rich water (HRW) could protect rats from hepatotoxicity caused by sub-chronic exposure to CPF. Materials and method. Rats were treated with hydrogen-rich water by oral intake for 8 weeks. Biochemical indicators of liver function, SOD and CAT activity, GSH and MDA levels were determined by the spectrophotometric method. Liver cell damage induced by CPF was evaluated by histopathological and electron microscopy analysis. PCR array analysis was performed to investigated the effects of molecular hydrogen on the regulation of oxidative stress related genes. Results. Both the hepatic function tests and histopathological analysis showed that the liver damage induced by CPF could be ameliorated by HRW intake. HRW intake also attenuated CPF induced oxidative stress, as evidenced by restored SOD activities and MDA levels. The results of PCR Array identified 12 oxidative stress-related genes differentially expressed after CPF exposure, 8 of chich, including the mitochondrial Sod2 gene, were significantly attenuated by HRW intake. The electron microscopy results indicated that the mitochondrial damage caused by CPF was alleviated after HRW treatment. Conclusions. The results obtained suggest that HRW intake can protect rats from CPF induced hepatotoxicity, and the oxidative stress signaling and the mitochondrial pathway may be involved in the protection of molecular hydrogen.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 368-373
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Disrupted iron metabolism in peritoneal fluid may induce oxidative stress in the peritoneal cavity of women with endometriosis
    Autorzy:
    Polak, G.
    Barczyński, B.
    Wertel, I.
    Kwaśniewski, W.
    Bednarek, W.
    Derewianka-Polak, M.
    Fraszczak, K.
    Olajossy, M.
    Kotarski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081934.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Endometriosis
    peritoneal fluid
    oxidative stress
    haemaglobin
    iron
    total oxidative status
    total antioxidant status
    Opis:
    Introduction and objective. Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in the pathogenesis of endometriosis still remains inconsistent. The aim of the study was to determine iron metabolism markers and their influence on oxidative stress parameters in the peritoneal fluid of women with endometriosis. Materials and method. 110 women with endometriosis and 119 patients with benign ovarian cysts were included in the study. All visible peritoneal fluid was aspirated during laparoscopy from the anterior and posterior cul-de-sacs. under direct vision to avoid blood contamination. Haemoglobin, iron, total oxidative status, and total antioxidant status were measured using standard colourimetric kits. Results. Haemoglobin, iron levels, as well as total oxidative status values were significantly higher, whereas total antioxidant status values were significantly lower in the peritoneal fluid of patients with endometriosis, in comparison to the reference groups. No differences were observed in peritoneal fluid concentrations of all parameters measured in relation to the phase of the menstrual cycle. Conclusions. Peritoneal fluid of women with endometriosis is characterized by disrupted iron metabolism. This is most likely related to an increased number of erythrocytes in the peritoneal cavity of endometriotic women, which leads to a higher concentration of haemoglobin in this environment. Impaired iron homeostasis may have a significant influence on the pathophysiology of peritoneal endometriosis by the direct impact of haemoglobin derivatives and/or formation of the pro-inflammatory and pro-oxidative environment. Peritoneal cavity oxidative stress occurs predominantly in women in advanced stages of the disease.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 587-592
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of nitric oxide in paraquat-induced oxidative stress in rat striatum
    Autorzy:
    Djukic, M
    Jovanovic, M.C.
    Ninkovic, M.
    Vasiljevic, I.
    Jovanovic, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/49779.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Wistar rat
    brain
    nitric oxide
    paraquat
    oxidative stress
    pesticide
    herbicide
    neurotoxicity
    exposure
    rat
    Opis:
    The role of nitric oxide (NO) in paraquat (PQ)-induced neurotoxicity is still not fully understood. In this study we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective nitric oxide synthase (NOS) inhibitor, in order to examine the effects of NO, reactive oxygen species (ROS) generation and lipid peroxidation (LPO) development during PQ-mediated neurotoxicity. Oxidative stress development in the striatum of Wistar rats intrastriatally (i.s.) poisoned with PQ (and in some cases pre-treated with L-NAME) was investigated by measuring superoxide anion (O2 •ˉ), malondialdehyde (MDA) and nitrate (NO3ˉ), 30 min, 24 hours and 7 days after treatment. L-NAME pretreatment provided the possibility to distinguish the role of ROS from reactive nitrogen species (RNS) in oxidative stress development induced by PQ. Our results confi rm the involvement of NO in PQ-mediated neurotoxicity and reduced LPO by L-NAME pretreatment implying that the latter has a protective role.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers - a study in postmenopausal women
    Autorzy:
    Sagan, Dorota
    Stępniak, Jan
    Gesing, Adam
    Lewiński, Andrzej
    Karbownik-Lewińska, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/987005.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    smoking
    former-smokers
    never-smokers
    postmenopausal women
    oxidative stress
    lipid peroxidation
    skin biophysical
    characteristics
    sebum
    moisture
    elasticity
    Opis:
    Introduction and objective. Protective antioxidative effects of melatonin have been repeatedly documented in experimental and clinical studies. One of the most spectacular exogenous prooxidative agents is cigarette smoking. The aim of the study was to evaluate the level of oxidative damage to membrane lipids (lipid peroxidation; LPO) in blood serum, and in epidermis exfoliated during microdermabrasion collected from former-smokers who were treated with melatonin. Materials and Method. The study was performed in postmenopausal women. Ninety (90) female volunteers, aged 46–67 years, were enrolled. Two major groups, i.e. never-smokers (n=44) and former-smokers (n=46), were divided into: Control, melatonin topical skin application, Restructurer (containing antioxidants) topical skin application, and melatonin oral treatment. Microdermabrasion was performed at point ‘0’, after 2 weeks, and after 4 weeks of treatment. The following parameters were measured: LPO in blood serum, LPO in epidermis exfoliated during microdermabrasion, and skin biophysical characteristics, such as sebum, moisture, elasticity, and pigmentation. Malondialdehyde+4-hydroxyalkenals level (LPO index) was measured spectrophotometrically. Results. Melatonin oral treatment significantly reversed the increased serum LPO level in former-smokers already after 2 weeks of treatment. In a univariate regression model, LPO blood level constituted the only independent factor negatively associated with melatonin oral treatment. After 4 weeks of treatment, melatonin given orally increased skin sebum, moisture and elasticity levels, and melatonin applied topically increased sebum level. Conclusion. Exogenous melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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