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Tytuł:
Mechanizmy infekcji patogenami przenoszonymi przez kleszcze na przykładzie bakterii: Anaplasma phagocytophilum i Borrelia burgdorferi
Mechanisms of infection by pathogens transmitted by ticks on the example of bacteria: Anaplasma phagocytophilum and Borrelia burgdorferi
Autorzy:
Wróblewska, Paula
Adamczuk, Piotr
Galińska, Elżbieta Monika
Chmielewska-Badora, Jolanta
Zwoliński, Jacek
Chmielewski, Jarosław
Powiązania:
https://bibliotekanauki.pl/articles/1035574.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
"Anaplasma phagocytophilum"
"Borrelia burgdorferi"
"kleszcze"
"mechanizmy infekcji"
Opis:
Tick-borne diseases are transmission diseases belonging to the group of zoonoses but carried by ticks. These diseases are a major public health problem but also a problem for groups occupationally exposed to tick bites. Ixodes ricinus is a species of ticks which is the most common reservoir and the vector of a large number of microorganisms pathogenic to humans. It transfers, among others, bacteria of the species: Anaplasma phagocytophilum and Borrelia burgdorferi. The article discusses the mechanisms of infection with Borrelia burgdorferi and Anaplasma phagocytophilum for both ticks as well as for animals and humans. The two microorganisms discussed have developed many characteristics and mechanisms of adaptation to the environment, as well as defense mechanisms against the body's immune response. Understanding the biology of ticks and the function of proteins produced by ticks and pathogenic microorganisms is the key in the development of effective treatments and prevention of Lyme disease and anaplasmosis.
Choroby odkleszczowe są chorobami transmisyjnymi, należącymi do grupy chorób odzwierzęcych, ale przenoszonych za pośrednictwem kleszczy. Choroby te stanowią ważny problem zarówno zdrowia publicznego, ale także problem dla grup zawodowo narażonych na ukłucia kleszczy. Ixodes ricinus jest gatunkiem kleszcza, który jest najczęstszym rezerwuarem i wektorem licznych mikroorganizmów wywołujących choroby ludzi. Przenosi on między innymi bakterie z gatunków: Anaplasma phagocytophilum i Borrelia burgdorferi. W artykule zostały omówione mechanizmy infekcji Borrelia burgdorferi i Anaplasma phagocytophilum zarówno kleszcza, ale także zwierząt i ludzi. Obydwa omawiane mikroorganizmy wykształciły wiele cech i mechanizmów przystosowawczych do środowiska, a także mechanizmów obronnych przed odpowiedzią immunologiczną organizmu. Poznanie biologii kleszczy, funkcji białek wytwarzanych przez kleszcze i patogenne mikroorganizmy stanowi klucz w opracowaniu skutecznych metod leczenia i profilaktyki boreliozy i anaplazmozy.
Źródło:
Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 2; 63-68
1505-7054
2084-6312
Pojawia się w:
Medycyna Środowiskowa - Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland
Autorzy:
Asman, M.
Witecka, J.
Solarz, K.
Zwonik, A.
Szilman, P.
Powiązania:
https://bibliotekanauki.pl/articles/2085175.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Ixodes ricinus
Opolskie Province
Polska
Borrelia burgdorferi sensu lato
Anaplasma phagocytophilum
Babesia microti
Borrelia burgdorferi sensu lato genospecies
Opis:
Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis. Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland. Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively. Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 544-547
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses
Autorzy:
Ebani, V.V.
Bertelloni, F.
Pinzauti, P.
Cerri, D.
Powiązania:
https://bibliotekanauki.pl/articles/989753.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Tematy:
leptospira spp.
borrelia burgdorferi sensu lato
horse
mat
ifat
Opis:
The aim of the study was to determine the seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in healthy horses living in 7 provinces of central Italy. In the period 2007-2009, sera from 386 horses were tested by microagglutination test (MAT) to detect antibodies to Leptospira spp., employing the following serovars as antigens: Bratislava, Ballum, Canicola, Icterohaemorrhagiae, Grippotyphosa, Hardjo, Pomona, Tarassovi. 3 animals were positive for the serovars Icterohaemorrhagiae, 2 to Bratislava, and 1 to Pomona, for a total 1.5% seroprevalence. All sera were examined by immunofluorence antibody test (IFAT) to reveal anti-B. burgdorferi s.l. antibodies. 94 (24.3%) horses were positive with antibody titres ranging from 1:64 to 1:1,024. The seroprevalence was significantly higher in >10 year-old horses compared to younger subjects. No significant differences in the mean seroprevalence were observed in the respective years. The total mean seroprevalence were strictly related to the environmental conditions of the areas in which the horses lived. No crossreactions between Leptospira and Borrelia were observed. This is the first serological survey on antibodies to B. burgdorferi s.l. in Italian horses.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Borrelia burgdorferi in ticks removed from skin of people and circumstances of being bitten - research from the area of Poland, 2012–2014
Autorzy:
Gałęziowska, E.
Rzymowska, J.
Najda, N.
Kołodziej, P.
Domzal-Drzewicka, R.
Rzaca, M.
Muraczyńska, B.
Charzyńska-Gula, M.
Szadowska-Szlachetka, Z.
Ślusarska, B.
Guty, E.
Powiązania:
https://bibliotekanauki.pl/articles/2081592.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
ticks
Borrelia burgdorferi
tick- borne disease
Lyme disease
tick’s bite
Opis:
Introduction. During feeding, the tick sucks blood from the host along with the pathogens that are in the blood, simultaneously exchanging its own pathogens with the host. Humans can also be a host. It is important to understand the most typical circumstances in which people might become infected with Borrelia burgdorferi. This knowledge will help to prepare health education programmes aimed at the prevention of Lyme disease and other tick-borne diseases. Objective. The aim of the study was to determine the percentage of ticks infected with Borrelia burgdorferi sensu lato, depending on the circumstances of getting bitten. Materials and method. The research material consisted of ticks acquired from people who had been bitten, and questionnaires completed by these people. 510 ticks were acquired from 257 females and 253 males. Following delivery of a tick for testing, the stage of its development was determined and a molecular assay of Borrelia burgdorferi DNA performed. Results. A positive result of the nested-PCR test was obtained in 78 ticks, which represents 15.30% of all ticks. The infected ticks were collected from male (41 ticks – 52.56%) and female subjects (37 ticks – 47.44%). The biggest number of infected ticks were collected in autumn (54 ticks – 69.23%) and from people who had been into forests (44 ticks – 56.41%). Among the people from whom the infected ticks were acquired, the dominating group included persons over 16 years of age (53 persons – 67.95%) and children aged 0–5 years (16 persons – 20.51%). One in four infected ticks were acquired from the southwestern (20 ticks – 25.64%) and eastern regions of Poland (21 ticks – 26.92%). Conclusion. Infestation of ticks infected with Lyme disease spirochete in this study proved to be variable and depend on the season, the area of tick attack and the region in Poland. The results of the study clearly show that ticks infected with Borrelia burgdorferi inhabit all regions of Poland. The results are consistent with National Institute of Hygiene data which indicates that Lyme disease cases are recorded in all regions of Poland.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 31-35
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First molecular evidence of Borrelia burgdorferi sensu lato in goats, sheep, cattle and camels in Tunisia
Autorzy:
Ben Said, Mourad
Belkahia, Hanène
Alberti, Alberto
Abdi, Khaoula
Zhioua, Manel
Daaloul-Jedidi, Monia
Messadi, Lilia
Powiązania:
https://bibliotekanauki.pl/articles/989299.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
borrelia burgdorferi sensu lato
ruminant species
molecular evidence
risk factors
tunisia
Opis:
Borrelia burgdorferi sensu lato (s.l.) are tick-transmitted spirochaetes of veterinary and human importance. Molecular epidemiology data on ruminants are still lacking in most countries of the world. Therefore, the aim of this study was to estimate the rate of B. burgdorferi s.l. infection in ruminants from Tunisia. A total of 1,021 ruminants (303 goats, 260 sheep, 232 cattle and 226 camels) from different bioclimatic areas in Tunisia were investigated for the presence of B. burgdorferi s.l. DNA in blood by real time PCR. Prevalence rates were 30.4% (92/303) in goats, 6.2% (16/260) in sheep, 1.3% (3/232) in cattle, and 1.8% (4/226) in camels. Only tick species belonging to Rhipicephalus and Hyalomma genera were found on the investigated animals. In small ruminants, the prevalence of B. burgdorferi s.l. varied significantly according to localities and farms. Goats located in humid areas were statistically more infected than those located in sub-humid areas. Prevalence rates varied significantly according to age and breed in sheep, and age and tick infestation in goats. This study provides the first insight into the presence of B. burgdorferi s.l. DNA in ruminants in Tunisia, and demonstrates that host species such as goats and sheep may play an important role in natural Lyme disease cycles in this country.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of cases of borreliosis certified as an occupational disease in the province of Wielkopolska [Poland]
Autorzy:
Bilski, B
Powiązania:
https://bibliotekanauki.pl/articles/49798.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Wielkopolska region
occupational disease
occurrence
borreliosis
epidemiology
Borrelia burgdorferi
agriculture
forestry
worker
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 211-217
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion
Autorzy:
Wojcik-Fatla, A
Szymanska, J.
Wdowiak, L.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51180.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick
Ixodes ricinus
coincidence
pathogen
Borrelia burgdorferi
Anaplasma phagocytophilum
Babesia microti
Lublin macroregion
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 151-158
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological evidence of exposure to zoonotic tick-borne bacteria in pheasants (Phasianus colchicus)
Autorzy:
Ebani, Valentina Virginia
Bertelloni, Fabrizio
Mani, Paolo
Powiązania:
https://bibliotekanauki.pl/articles/988938.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
pheasant (phasianus colchicus)
anaplasma phagocytophilum
borrelia burgdorferi s.l.
coxiella burnetii
sfg rickettsia spp.
ifat
Opis:
Introduction and objective. Previous studies on tick-borne pathogens in the avian population have focused mainly on the detection of the agents in ticks collected from birds, but data about the presence of tick-borne bacteria in these animals are scant. The aim of the presented study was to verify the exposure to some zoonotic tick-borne bacteria, in particular, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Coxiella burnetii, and the Rickettsia species of the Spotted Fever Group (SFG), in pheasants (Phasianus colchicus) living in a central Italy area, characterized by conditions favourable for the diffusion of the ticks. Materials and method. Blood serum samples from 276 farm-reared pheasants were examined by indirect immunofluorescence antibody test to detect antibodies against the 4 reported pathogens. Results. A total of 124 (44.92%) birds resulted as seropositive: in particular 3 (1.08%) to C. burnetii, 31 (11.23%) to A. phagocytophilum, 46 (16.67%) to B. burgdorferi s.l., 49 (17.75%) to SFG Rickettsia spp. antigens. Three pheasants resulted positive both to A. phagocytophilum and B. burgdorferi s.l. antigens, whereas 2 – both to B. burgdorferi s.l. and SFG Rickettsia spp. antigens. The antibody titers varied from 1:40–1:320. Conclusions. The obtained results show that the pheasants had developed antibodies to the investigated tick-borne agents. For this reason, they seem to be involved in the epidemiology of the studied tick-borne bacteria.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
Autorzy:
Cisak, E
Chmielewska-Badora, J.
Zwolinski, J.
Wojcik-Fatla, A.
Zajac, V.
Skorska, C.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51535.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
borreliosis
Polska
rural inhabitant
Lyme borreliosis
tick
seroepidemiology
Ixodes ricinus
Borrelia burgdorferi
clinical case
Lublin region
Źródło:
Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vestibular rehabilitation after unilateral neuritis in the course of neuroborreliosis - a case report
Autorzy:
Krasowska, S.
Powiązania:
https://bibliotekanauki.pl/articles/2097826.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lyme disease
Borrelia burgdorferi
rehabilitation
Unilateral prolapse of the labyrinthine function
vestibular neuritis
vestibular compensation
Opis:
Inflammation of the vestibular nerve (vestibular neuronitis, VN) is the third most common reason of peripheral vertigo.Many VN reasons lead to a thorough approach to the patient and diagnostics based on excluding even the rarest causes. Such a cause may be infection with Borrelia burgdorferi, which, due to high tropism to the nervous system, damages the sheath of nerve cells, causing inflammation. The acute course of the disease provokes the use of standard treatment in the form of pharmacotherapy e.g.: with neuroleptics, anxiolytics and then beta-blockers, betahistine. The literature, however, clearly indicates the limitations of pharmacology, which weaken the vestibular compensation, prompting the rapid discontinuation of drugs and the commencement of rehabilitation. This publication presents a case report of a 37-year-old female patient diagnosed with VN and Lyme disease. The case is to present and analyze the progress of vestibular rehabilitation along with the accompanying causal treatment.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2020, 14, 3; 85-89
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans
Autorzy:
Kondrusik, M
Grygorczuk, S.
Skotarczak, B.
Wodecka, B.
Rymaszewska, A.
Pancewicz, S.
Zajkowska, J.
Swierzbinska, R.
Hermanowska-Szpakowicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/51856.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
human disease
erythema migrans phase
patient
diagnosis
ELISA test
polymerase chain reaction
serological diagnosis
Borrelia burgdorferi
borreliosis
Opis:
The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of infections and co-infections with 6 pathogens in Dermacentor reticulatus ticks collected in eastern Poland
Autorzy:
Zając, Violetta
Wójcik-Fatla, Angelina
Sawczyn, Anna
Cisak, Ewa
Sroka, Jacek
Kloc, Anna
Zając, Zbigniew
Buczek, Alicja
Dutkiewicz, Jacek
Bartosik, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/989947.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
dermacentor reticulatus
co-infections
tbe virus
anaplasma phagocytophilum
rickettsia raoultii
borrelia burgdorferi sensu
lato
babesia spp.
toxoplasma gondii
Opis:
Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species. An attempt was made to determine the significance of the associations between 6 different pathogens occurring in D. reticulatus (Tick-borne encephalitis virus = TBEV, Anaplasma phagocytophilum, Rickettsia raoultii, Borrelia burgdorferi s. l., Babesia spp., Toxoplasma gondii), using 2 statistical methods: determination of Odds Ratios (ORs) and the Fisher’s exact test. 634 questing Dermacentor reticulatus ticks (370 females and 264 males) were collected in 2011–2013 by flagging the lower vegetation in 3 localities in the area of Łęczyńsko-Włodawskie Lakeland, situated in the Lublin region of eastern Poland. The presence of individual pathogens was detected by PCR. Ticks were infected most often with Rickettsia raoultii (43.8%), less with TBEV (8.5%), and much less with Babesia spp., Toxoplasma gondii, Borrelia burgdorferi s.l., and Anaplasma phagocytophilum (2.5%, 2.1%, 1.6% and 1.1%, respectively). The locality-dependent variability proved to be significant for TBEV (c2=11.063; P=0.004) and Toxoplasma gondii (c2=11.298; P=0.0035), but not for other pathogens. Two hundred seventy (42.6%) of the examined ticks were infected only with a single pathogen, and 54 (8.5%) showed the presence of dual co-infections, each with 2 pathogens. The most common were dual infections with participation of Rickettsia raoultii (7.41%); next, those with participation of the TBEV (5.21%), Toxoplasma gondii (1.58%), Borrelia burgdorferi s.l. (1.26%), Anaplasma phagocytophilum (0.95%), and Babesia spp. (0.63%). On the total number of 15 possible associations, in 9 cases co-infections occurred whereas in 6 cases they were not detected. The most noteworthy were positive co-infections with the participation of TBEV, which proved to be weakly significant (0.05
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus from the city of Brno, Czech Republic
Autorzy:
Pejchalova, K
Zakovska, A.
Mejzlikova, M.
Halouzka, J.
Dendis, M.
Powiązania:
https://bibliotekanauki.pl/articles/49816.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
cultivation
infection
Brno
infection risk
Lyme borreliosis
Czech Republic
isolation
polymerase chain reaction
Borrelia burgdorferi
Ixodes ricinus
tick
identification
Opis:
A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected by fl agging of vegetation in suburban woods of Pisárky Park (city of Brno) from July to October 2002. The midgut of each tick was dissected out and transferred individually into BSK-H medium. After cultivation, all specimens were examined by dark-fi eld microscopy (DFM) for the presence of borreliae. Out of 305 tick samples, 45 were (14.8%) DFM positive. The following polymerase chain reaction (PCR) then revealed 37 (12.1%) samples positive for the presence of Borrelia burgdorferi sensu lato DNA. All 37 samples were further analysed by restriction fragment length polymorphism (RFLP) method. PCR-RFLP analysis revealed 14 strains of B. afzelii (37.8%), 15 strains of B. garinii (40.5%) and 2 strains of B. burgdorferi sensu stricto (5.4%). Four samples (10.8%) showed a mixed population of these genospecies. Two samples produced atypical RFLP pattern which were detected by sequence analysis as B. valaisiana (5.4%). Isolation attempts resulted in 21 spirochaetal strains (including two stains of B. valaisiana). The results show the diversity of B. burgdorferi s.l. in tick population and refer the risk of infection by pathogenic borreliae in Brno.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
Autorzy:
Sroka, J
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/51137.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
tick
Ixodes ricinus
Toxoplasma gondii
Borrelia burgdorferi
occurrence
polymerase chain reaction
genotyping
B1 gene
human population
infection
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
What is percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?
Autorzy:
Nejedla, P
Norek, A.
Vostal, K.
Zakovska, A.
Powiązania:
https://bibliotekanauki.pl/articles/51950.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
mosquito
larva
Culex
spirochaete
polymerase chain reaction
Borrelia burgdorferi
Culex pipiens
human disease
tick
Ixodes ricinus
midgut
Czech Republic
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 273-276
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 18

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