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    Wyświetlanie 1-3 z 3
    Tytuł:
    Evaluation of acute low back pain in women after treatment with interferential current
    Autorzy:
    Serafim, A.
    Rabel, J.
    Neves, M.
    Da Silva, T.S.
    Bertolini, G.R.F.
    Powiązania:
    https://bibliotekanauki.pl/articles/3294.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2019, 13, 2
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First detection and molecular characterization of hepatitis E virus in water from wastewater treatment plants in Portugal
    Autorzy:
    Matos, A.
    Mesquito, J.R.
    Goncalves, D.
    Abreu-Silva, J.
    Luxo, C.
    Nascimento, M.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081721.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    wastewater
    hepatitis E virus
    genotype 3
    zoonotic
    Opis:
    Hepatitis E virus (HEV) genotype 3 is widespread in industrialized countries and widely recognized as a serious public health issue. HEV genotype 3 is excreted in both human and animal stools, having the potential to be introduced in aquatic environments through wastewater discharge. For this reason, waterborne transmission of the HEV genotype 3 is drawing increasing attention from the scientific community. Although studies in Portugal have shown that HEV circulates in both humans and swine, no study has yet focused on the presence of HEV in wastewater. Hence, a total of 60 water samples (influent and effluent) from 15 Wastewater Treatment Plants (WWTP) of different regions of Portugal were tested for the presence of HEV using a broad spectrum real-time RT-PCR with amplification within the ORF2 region of HEV genome. Positive samples were retested by nested RT-PCR with amplification within the ORF1 region, sequenced followed by phylogenetic analysis. HEV sequences were retrieved from two influent samples collected in December 2013 from WWTPs of North and Central of Portugal and classified in subgenotype 3i and 3f. This is the first study showing the presence of HEV in aquatic environments of Portugal.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 364-367
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular evidence of hepadnavirus infection in swine
    Autorzy:
    Vieira, Y.R.
    Silva, M.F.M.
    Santos, D.R.L.
    Vieira, A.A.
    Ciacci-Zanella, J.R.
    Barquero, G.
    do Lago, B.V.
    Gomes, S.A.
    Pinto, M.A.
    de Paula, V.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/989989.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    diagnostic
    hepadnavirus
    swine
    Opis:
    Introduction and objective. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously. Materials and methods. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing. Results. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8–96.3% of identity. Conclusions. Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2015, 22, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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