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Wyświetlanie 1-7 z 7
Tytuł:
Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Autorzy:
Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.
Powiązania:
https://bibliotekanauki.pl/articles/51141.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray
Opis:
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater
Autorzy:
Cornejova, T.
Venglovsky, J.
Gregova, G.
Kmetova, M.
Kmet, V.
Powiązania:
https://bibliotekanauki.pl/articles/50050.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2015, 22, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse
Autorzy:
Gregova, G.
Kmetova, M.
Kmet, V.
Venglovsky, J.
Feher, A.
Powiązania:
https://bibliotekanauki.pl/articles/52172.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Extra-intestinal pathogenic Escherichia coli – threat connected with food-borne infections
Autorzy:
Wasiński, B.
Powiązania:
https://bibliotekanauki.pl/articles/2085200.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
food
food-borne pathogens
extra-intestinal pathogenic escherichia coli
ExPEC
Opis:
Infections caused by extra-intestinal pathogenic Escherichia coli (ExPEC) are a serious public health problem worldwide. The most troublesome are urinary tract infections, severe neonatal meningitis, serious intraabdominal infections, and more rarely, pneumonia, intravascular-device infections, osteomyelitis, soft-tissue infections or sometimes bacteraemia. These strains are also able cause significant economic losses in animal husbandry. A thorough understanding of ExPEC ecology, reservoirs, chains and dynamics of transmission can greatly contribute to a reduction in the burden of ExPEC-associated disease. The ability of E. coli (including ExPEC) to exist and survive in various ecological niches impedes the precise recognition and indication of transmission routes most important for individual infections cases. Among many identified ExPEC reservoirs, animal companion and animals providing food seem to be important sources of infection for human; however, the real level of risk connected with potential transmission of these bacteria remains unclear. Food is indicated as one of potential ways of transmission. Despite a quite high number of reports, many of the uncertainties are expected to be reliably elucidated. This review presents most important data on the current state of knowledge concerning the potential role of food in ExPEC transmission. The possible consequences of ExPEC infections in human and animals are briefly described.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 532-537
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens – clinical submissions
Autorzy:
Majewski, M.
Józefiak, A.
Kimsa-Furdzik, M.
Dziubdziela, L.
Hudak-Nowak, M.
Wilczyński, J.
Anusz, K.
Powiązania:
https://bibliotekanauki.pl/articles/28762783.pdf
Data publikacji:
2021
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2021, 28, 2; 271-276
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sanitary condition of electrical hand dryers in toilets of shopping centres in Kraków
Stan sanitarno-higieniczny elektrycznych suszarek do rąk w toaletach centrów handlowych na terenie miasta Krakowa
Autorzy:
Lenart-Boroń, Anna
Jarosiński, Jordan
Powiązania:
https://bibliotekanauki.pl/articles/1177579.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Tematy:
"Escherichia coli"
"gronkowce"
"mikroorganizmy w powietrzu"
"powietrze"
Opis:
Introduction. Shopping habits have changed over the past several years and consumers choose shopping centres, where they can spend time and visit restaurants. People usually wash hands before meals, but toilets in shopping centres are visited by hundreds of users every day which= makes their cleanliness questionable. Therefore, the aim of this study was to assess the sanitary condition of electrical hand dryers in toilets of the selected shopping centres in Kraków. Material and methods. Air was sampled at the outlet of electrical hand dryers and surface swabs of hand dryers were taken in toilets of five shopping centres. Air sampling was conducted using the MAS-100 impactor. The number of mesophilic bacteria, fungi, actinomycetes, staphylococci and Escherichia coli was determined. The results of air analyses were compared with the limits provided by the Polish Standards on air quality. Results. The number of microorganisms varied between the studied centres. In terms of mesophilic bacteria and fungi the air was unpolluted. The concentration of actinomycetes however significantly exceeded the limits set by the Polish Standards. Numerous Escherichia coli were found both in the air and in swabs in one of the centres. However the number of E. coli and staphylococci did not exceed the minimum infective dose. Conclusions. The studies showed high concentrations of airborne actinomycetes and staphylococci. Also the presence of E. coli in the air and swabs allows the conclusion that legal regulations for concentrations of airborne microorganisms are necessary to make people aware of this problem. However, it was found that using electrical hand dryers does not have impact on human health.
Wstęp. W przeciągu ostatnich lat zwyczaje konsumentów znacznie się zmieniły i klienci coraz częściej rezygnują z osiedlowych sklepów na rzecz centrów handlowych, w których mogą spędzić czas, a także skorzystać z bogatych ofert restauracji. Wielu ludzi odruchowo myje ręce przed posiłkami, jednak toalety w centrach handlowych odwiedzane są przez setki osób dziennie, a ich czystość może być wątpliwa. Celem badań była ocena stanu sanitarno-higienicznego suszarek do rąk w toaletach wybranych centrów handlowych w Krakowie. Materiały i metody. Pobrano powietrze u wylotu suszarek elektrycznych oraz wymazy powierzchniowe wylotów powietrza w suszarkach w toaletach pięciu centrów handlowych. Próbki powietrza pobrano przy użyciu impaktora MAS-100. Oznaczono liczebność bakterii mezofilnych, grzybów, promieniowców, gronkowców i Escherichia coli. W przypadku badań powietrza wyniki porównano z zaleceniami Polskich Norm dotyczących jakości powietrza atmosferycznego. Wyniki. Liczebność drobnoustrojów w badanych centrach handlowych była zróżnicowana. Pod względem liczby bakterii mezofilnych i grzybów powietrze we wszystkich toaletach było niezanieczyszczone. Stężenie promieniowców znacznie przekraczało wartości dopuszczalne w Polskich Normach. Również liczba gronkowców była bardzo wysoka. W jednym z badanych centrów stwierdzono liczne bakterie Escherichia coli, zarówno w powietrzu, jak i w wymazach. Jednak liczebność zarówno E. coli jak i gronkowców nie przekroczyła granicy minimalnej dawki infekcyjnej. Wnioski. W badaniach stwierdzono wysokie stężenia promieniowców i gronkowców. Obecność E. coli w powietrzu i w suszarkach daje podstawę do stwierdzenia, że normy prawne regulujące stężenie mikroorganizmów w powietrzu są potrzebne, aby podkreślić znaczenie problemu. Stwierdzono jednakże, że korzystanie z elektrycznych suszarek do rąk nie zagraża zdrowiu użytkowników.
Źródło:
Medycyna Środowiskowa - Environmental Medicine; 2015, 18, 2; 32-38
1505-7054
2084-6312
Pojawia się w:
Medycyna Środowiskowa - Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of the Filter-Sandwich carriers in continuous effectiveness monitoring of slurry treatment methods as an element improving biosafety in agriculture
Autorzy:
Skowron, K.
Olszewska, H.
Paluszak, P.
Skowron, K.J.
Bauza-Kaszewska, J.
Powiązania:
https://bibliotekanauki.pl/articles/50039.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
Filter-Sandwich carrier
effectiveness
monitoring
slurry
treatment method
biosafety
agriculture
slurry storage
pathogen
Salmonella
Escherichia coli
Campylobacter
physicochemical analysis
Opis:
Slurry, due to high microbiological contamination, requires hygienization before spreading. The agricultural usage of treated slurry has to guarantee biosafety. Therefore, constant monitoring of the slurry treatment process should be conducted. The use of Filter-Sandwich carriers seems to be a prospective solution. The aim of the research was to test whether Filter-Sandwich carriers influence the survivability of microorganisms during the slurry hygienization process and hence, whether they are safe for the environment. Raw cattle and swine slurry with different dry matter content was the research material. Salmonella Senftenberg W775 rods were introduced directly into the slurry and into the carriers placed in the liquid excrements stored at 4 and 20 °C, and underwent anaerobic digestion at 35 °C. The number of tested bacteria obtained from the slurry and carriers was determined using the MPN method with proper microbiological media. The values of physicochemical parameters of the raw and treated slurry were determined, both for the carriers and for slurry only. Biosafety control was also conducted for the carriers in slurry containers. The differences in the theoretical survivability between Salmonella Senftenberg W775 re-isolated from the slurry and the carriers, and in the values of the selected physicochemical parameters obtained at the end of the process, were not statistically significant. The re-contamination of the sterile slurry caused by the bacteria in the carrier was not observed after placement of the carrier with inoculated material. The conducted research proves the usefulness of Filter-Sandwich carriers for continuous hygienization monitoring of the slurry treatment process. This refers not only to the semi-technical scale, but also to the full-scale process.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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