- Tytuł:
- Molecular probes for detection of wheat chromosomal fragments in rye.
- Autorzy:
-
Rafalski, Andrzej
Wiśniewska, Iwona
Sikora, Teresa
Łapiński, Bogusław - Powiązania:
- https://bibliotekanauki.pl/articles/2198780.pdf
- Data publikacji:
- 2000-12-20
- Wydawca:
- Instytut Hodowli i Aklimatyzacji Roślin
- Tematy:
-
introgression
PCR
rye
Southern blotting
Triticum durum probe
wheat
wheat specific probes - Opis:
- Twelve DNA fragments amplified on templates of Triticum urartu, Aegilops speltoides and Aegilops squarrosa were separated, reamplified and used as digoxigenin-labelled probes. The species and genome specificity of probes was evaluated by Southern dot-blot hybridisation to Triticum durum, the donors of wheat genomes, rye and rye with introgressed wheat chromosome fragments. In comparison to labelled genomic DNA of T. durum, the probes obtained by labelling PCR-amplified fragments exhibited higher specificity to wheat genomes. Under the applied hybridisation conditions all probes showed different degree of cross-hybridisation to rye DNA. Some probes indicated quantitative difference in their specificity to wheat genomes, but generally the intensity of hybridisation to the genomes A, S and D was independent of the origin of an amplified fragment. Selected probes, used in dot-blot hybridisation system together with genomic DNA of T. durum, may increase the sensitivity of screening wheat chromosomal fragments introgressed to rye.
- Źródło:
-
Plant Breeding and Seed Science; 2000, 44; 27-37
1429-3862
2083-599X - Pojawia się w:
- Plant Breeding and Seed Science
- Dostawca treści:
- Biblioteka Nauki
Artykuł