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Wyświetlanie 1-3 z 3
Tytuł:
Tetraploid triticale as a potential source of new variation for rye
Autorzy:
Łapiński, Bogusław
Rafalski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/2198908.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
chromosome translocation
introgression
rye
tetraploid triticale
Opis:
High crossability of tetraploid triticale (X Triticosecale Wittmack) with rye (Secale cereale L.) and relatively high fertility of the resulting hybrids make the triticale an attractive bridge species for introduction of wheat genes into rye breeding populations. It was found, with the use of in situ hybridization technique, that some 4x triticale materials bred in Radzików contain small wheat translocations, of both distal and intercalary type, into the rye 5R chromosome. The distal wheat translocation occupying less than 5% of the long arm was transferred into diploid rye, but a disomic line has not been established yet. Other wheat translocations of the chromosomes 1R and 5R were found in hybrids of 4x rye with 4x triticale. Besides the intergenomic crossing-over, at least one another mechanism of DNA rearrangements operated. Small intercalary two-dot signals of the wheat fluorescent probe were also found in one line on a rye chromosome different than 5R. Most puzzling was the „invisible” migration of wheat DNA to rye chromosomes, detectable on southern dot-blots, but not on the in situ slides. The wheat probe dot-blot signals were recorded for more than 1/3 of rye plants from the first back-cross of the 4x triticale × 2x rye hybrids to rye.
Źródło:
Plant Breeding and Seed Science; 2003, 48; 25-35
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Location of markers of aluminium tolerance genes on rye chromosomes (Secale cereale L.)
Autorzy:
Wiśniewska, Iwona
Rafalski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/2199029.pdf
Data publikacji:
2006-06-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
aluminium tolerance
chromosomal location
DNA-DNA hybridisation
PCR markers
wheat/rye addition lines
Opis:
The aim of presented work was to identify of PCR amplifiedDNAfragments differentiating aluminium tolerant and sensitive forms of rye and to locate the markers on rye chromosomes. For identification of markers, the PCR system with semi-specific primers targeting intron-exon sequences of plant genes was applied. The modified method of bulked segregant analysis was used. The pooled DNAs of two or three F2 segregating populations were screened together with DNA of their parental inbred lines. Potential marker of tolerance gene was located on rye chromosomes using wheat/rye (Chinese Spring/Blanco) additional lines. The specific probes obtained from DNA fragments differentiating sensitive and tolerant forms of rye were hybridized to PCR amplified DNA fragments of sensitive and tolerant forms of rye and the set of wheat/rye addition lines. Independently of the method of digoxygenin labelling (primer extension or Taq polymerase reaction), the probes obtained showed similar hybridization patterns. The results of hybridisation of 21 probes prepared from 12 DNA fragments confirmed connection of selected DNA fragments with to aluminium tolerance or sensitivity. Most of these DNA fragments originated from tolerant forms of rye. Using this method it was possible to locate eight DNA fragments on rye chromosomes. Three DNA fragments hybridised to chromosome 4R, two DNA fragments to chromosome 6R and single DNA fragments to chromosomes 1R, 2R and 3R. Four DNA fragments indicating clear relationship with character studied were not located on particular chromosomes using this set of wheat/rye addition lines. Hybridisation of probes prepared from four DNA fragments revealed length polymorphism. Probes prepared from two DNA fragments were characterised as dominant markers. In other cases the type of marker (dominant/codominat) was not fully documented.
Źródło:
Plant Breeding and Seed Science; 2006, 53; 43-52
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular probes for detection of wheat chromosomal fragments in rye.
Autorzy:
Rafalski, Andrzej
Wiśniewska, Iwona
Sikora, Teresa
Łapiński, Bogusław
Powiązania:
https://bibliotekanauki.pl/articles/2198780.pdf
Data publikacji:
2000-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
introgression
PCR
rye
Southern blotting
Triticum durum probe
wheat
wheat specific probes
Opis:
Twelve DNA fragments amplified on templates of Triticum urartu, Aegilops speltoides and Aegilops squarrosa were separated, reamplified and used as digoxigenin-labelled probes. The species and genome specificity of probes was evaluated by Southern dot-blot hybridisation to Triticum durum, the donors of wheat genomes, rye and rye with introgressed wheat chromosome fragments. In comparison to labelled genomic DNA of T. durum, the probes obtained by labelling PCR-amplified fragments exhibited higher specificity to wheat genomes. Under the applied hybridisation conditions all probes showed different degree of cross-hybridisation to rye DNA. Some probes indicated quantitative difference in their specificity to wheat genomes, but generally the intensity of hybridisation to the genomes A, S and D was independent of the origin of an amplified fragment. Selected probes, used in dot-blot hybridisation system together with genomic DNA of T. durum, may increase the sensitivity of screening wheat chromosomal fragments introgressed to rye.
Źródło:
Plant Breeding and Seed Science; 2000, 44; 27-37
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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