- Tytuł:
- Quantifying Transient Psychological Stress Using a Novel Technique: Changes to PMA-Induced Leukocyte Production of ROS In Vitro
- Autorzy:
-
Shelton-Rayner, G. K.
Mian, R.
Chandler, S.
Robertson, D.
Macdonald, D. W. - Powiązania:
- https://bibliotekanauki.pl/articles/90414.pdf
- Data publikacji:
- 2011
- Wydawca:
- Centralny Instytut Ochrony Pracy
- Tematy:
-
chemiluminescence
humans
leukocytes
luminol
momentary confusion
psychological stress
reactive oxygen species - Opis:
- Although much work has been conducted to quantify the long-term physiological effects of psychological stress, measures of short-term, low-level stress have been more elusive. This study assessed the effect of exposure of volunteers to a mild, brief, psychologically stressful event, on the functional ability of leukocytes in whole blood to respond to phorbol 12-myristate 13-acetate (PMA) in vitro. Volunteers operated a car electric window and adjusted it to 4 pre-determined positions. Between each operation the mechanism’s polarity was covertly altered (group B) or remained unaltered (group A). For each treatment group 10 different subjects provided capillary blood samples pre- and post-stressor. Using a chemiluminescent technique termed leukocyte coping capacity, the ability of leukocytes to produce reactive oxygen species (ROS) in vitro was assessed. ROS release differed significantly at 10 min post-stressor between treatment groups, suggesting exposure to acute psychological stress leads to a reduced ability to respond to bacterial challenge.
- Źródło:
-
International Journal of Occupational Safety and Ergonomics; 2011, 17, 1; 3-13
1080-3548 - Pojawia się w:
- International Journal of Occupational Safety and Ergonomics
- Dostawca treści:
- Biblioteka Nauki
Artykuł