- Tytuł:
- Kinetic properties of Lipase obtained from Pseudomonas aeruginosa isolated from crude oil contaminated soil
- Autorzy:
-
Femi-Ola, Titilayo Olufunke
Orjiakor, Paul Ikechukwu
Enerijiofi, Kingsley Erhons
Oke, Iyanuoluwa Omolade
Fatoyinbo, Adebayo Adeola - Powiązania:
- https://bibliotekanauki.pl/articles/134985.pdf
- Data publikacji:
- 2019
- Wydawca:
- Akademia Morska w Szczecinie. Wydawnictwo AMSz
- Tematy:
-
biodegradation
enzymes
gel filtration
lipase
olive oil
Pseudomonas aeruginosa - Opis:
- Soil contaminated with crude oil has negatively affected some of the communities in Ekiti State region in Nigeria. There is an increased interest in microbial lipases because of their enormous potential in industrial and biotechnological applications. This study investigated the characteristics of partially-purified lipase from Pseudomonas aeruginosa isolated from oil-contaminated soil in Ado-Ekiti, Ekiti State. The lipase was purified by gel-filtration, having a molecular weight of 39.11 kDa, with Km and Vmax values of 12.50 and 28.86, respectively. The enzyme had an optimal pH of 8.0 and exhibited its maximal activity at 50°C, whereas the relatively stable temperature and pH were 40°C and 5.0, respectively. The enzyme activity was enhanced by olive oil, which served as the carbon source. Sodium chloride enhanced lipase activity, while calcium chloride acted as mild inhibitor, and iron chloride acted a strong inhibitor. The lipase from Pseudomonas aeruginosa possessed properties of an industrial enzyme and will be useful for biodegradation and bioremediation studies.
- Źródło:
-
Zeszyty Naukowe Akademii Morskiej w Szczecinie; 2019, 59 (131); 154-161
1733-8670
2392-0378 - Pojawia się w:
- Zeszyty Naukowe Akademii Morskiej w Szczecinie
- Dostawca treści:
- Biblioteka Nauki
Artykuł