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    Wyświetlanie 1-5 z 5
    Tytuł:
    Investigation and vizualization of the cells grown on ceramic coating by electron microscopy techniques
    Autorzy:
    Karbowniczek, J.
    Gruszczyński, A.
    Kruk, A
    Czyrska-Filemonowicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/285222.pdf
    Data publikacji:
    2017
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biocompatibility
    biomaterials
    scanning electron microscopy
    Źródło:
    Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 37
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microscopic analysis of the nanostructures impact on endothelial cells
    Autorzy:
    Kołodziejczyk, Agnieszka Maria
    Kucińska, Magdalena
    Jakubowska, Aleksandra
    Siatkowska, Małgorzata
    Sokołowska, Paulina
    Kotarba, Sylwia
    Makowski, Krzysztof
    Komorowski, Piotr
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844983.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanostructures
    atomic force microscopy
    transmission electron microscopy
    scanning electron microscopy
    cell morphology
    Opis:
    Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
    Źródło:
    Engineering of Biomaterials; 2020, 23, 154; 2-8
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microscopic analysis of the nanostructures impact on endothelial cells
    Autorzy:
    Kołodziejczyk, Agnieszka Maria
    Kucińska, Magdalena
    Jakubowska, Aleksandra
    Siatkowska, Małgorzata
    Sokołowska, Paulina
    Kotarba, Sylwia
    Makowski, Krzysztof
    Komorowski, Piotr
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/970974.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanostructures
    atomic force microscopy
    transmission electron microscopy
    scanning electron microscopy
    cell morphology
    Opis:
    Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
    Źródło:
    Engineering of Biomaterials; 2020, 23, 154; 2-8
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological and mechanical research of titanium implants covered with bactericidal coating
    Autorzy:
    Malisz, Klaudia
    Świeczko-Żurek, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/2172010.pdf
    Data publikacji:
    2022
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanometals
    scanning electron microscopy
    wettability
    nanoindentation
    antibacterial properties
    mechanical properties
    Opis:
    Materials used in bone implants should not only be non- -toxic to the surrounding tissues, but also should promote osseointegration and minimize the risk of infection. Infections are a serious problem contributing to implantation failure. They are associated with pain, immobilization, and the necessity of reoperation. In extreme cases, they can lead to significant inflammatory changes in the bones, which, in turn, can lead to amputation and even death. After implantation, the surrounding tissues are damaged. In addition, implants are susceptible to bacterial colonization due to the lack of microcirculation. Therefore, scientists are working on antibacterial coatings to prevent the adhesion of bacteria before tissue regeneration. The paper concerns the biological and mechanical properties of titanium implants with an antibacterial coating. The Ti13Zr13Nb alloy samples were coated with hydroxyapatite (HAp) coatings using the electrophoretic deposition technique (EPD). Subsequently, the surface of the samples was modified with silver, copper, and nickel nanoparticles by the immersion method. Different titanium sample types (i.e. HAp-only and nanometals-enriched coatings) were placed in a bacterial solution for a period of one month. Each sample was examined using scanning electron microscopy (SEM), nanoindentation, nanoscratch, and contact angle tests. The significant amount of dead biofilm on the surface proves the effectiveness of antibacterial activity. The wettability assessment showed that the samples were hydrophilic. The conducted tests of mechanical properties indicate the heterogeneity of the coatings.
    Źródło:
    Engineering of Biomaterials; 2022, 25, 165; 17--22
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
    Autorzy:
    Komorowski, Piotr
    Kołodziejczyk, Agnieszka
    Makowski, Krzysztof
    Kotarba, Sylwia
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844871.pdf
    Data publikacji:
    2021
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bacterial exopolysaccharides
    dextran- -based “microcarriers”
    scanning electron microscopy
    atomic force microscopy
    roughness parameters
    three-dimensional cell culture
    Opis:
    The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
    Źródło:
    Engineering of Biomaterials; 2021, 24, 159; 18-23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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