- Tytuł:
- Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
- Autorzy:
-
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V. - Powiązania:
- https://bibliotekanauki.pl/articles/285087.pdf
- Data publikacji:
- 2009
- Wydawca:
- Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
- Tematy:
-
Ar plasma discharge
biomaterials
low-density polyethylene
cell adhesion
cell proliferation
grafting
tissue engineering
vascular smooth muscle cells - Opis:
- Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
- Źródło:
-
Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
1429-7248 - Pojawia się w:
- Engineering of Biomaterials
- Dostawca treści:
- Biblioteka Nauki
Artykuł