Temat: emulsification - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Modification of microspheres’ microstructure for application as cell carriers in modular tissue engineering
Autorzy:: Mielan, B.
Krok-Borkowicz, M.
Pielichowska, K.
Pamuła, E.
Powiązania:: https://bibliotekanauki.pl/articles/285556.pdf
Data publikacji:: 2017
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: tissue engineering
polymers
emulsification
Źródło:: Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 72
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering
Autorzy:: Mielan, B.
Krok-Borkowicz, M.
Pielichowska, K.
Pamuła, E.
Powiązania:: https://bibliotekanauki.pl/articles/285127.pdf
Data publikacji:: 2017
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: regenerative medicine
cell cultures
bottom-up
PLGA
emulsification
Opis:: Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative surface area of MS provides more efficient cell culture environment than traditional culture on flat substrates (multiwell plates, Petri dishes). In addition, MS structure, topography and surface chemistry can be modified to promote cell adhesion and proliferation. The aim of this study was to obtain resorbable poly(L-lactide-co-glycolide) (PLGA) MS and to modify their properties by changing manufacturing conditions of the oil-in-water emulsification to better control structural and microstructural properties of MS and their biological performance. To this end, water phase was modified by addition of NaCl to change ionic strength, while oil phase by addition of polyethylene glycol (PEG). Microstructural and thermal properties were assessed. Cytocompatibility tests and cell cultures with MG-63 cells were conducted to verify potential relevance of MS as cell carriers. The results showed that it is possible to obtain cytocompatible MS by oil-in-water emulsification method and to control diameter, porosity and crystallinity of MS with the use of additives to oil and/or water phases without negative changes in MS cytocompatibility. The results prove that modification of both phases make it possible to produce MS with desired/controllable properties like surface topography, porosity and crystallinity.
Źródło:: Engineering of Biomaterials; 2017, 20, 140; 7-11
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Optimizing manufacturing conditions of polymer microspheres as cell carriers for modular tissue engineering
Autorzy:: Mielan, Bartosz
Pamuła, Elżbieta
Powiązania:: https://bibliotekanauki.pl/articles/1844978.pdf
Data publikacji:: 2020
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: modular tissue engineering
microspheres
cell culture
oil-in-water emulsification
poly(L-lactide-co-glycolide) (PLGA)
Opis:: Microspheres (MS) made of biostable polymer, namely polystyrene, have been used as substrates for cell culture enabling rapid cell expansion in dynamic conditions. However, due to non-resorbability, polystyrene (PS) MS when repopulated with cells cannot be directly used in tissue engineering. Our concept was to produce MS from resorbable polymer – poly(L-lactide- -co-glycolide) (PLGA) as a support for adherent cells, e.g. osteoblasts. We hypothesize that such MS can be applied to the injured site to act as cell carriers or as modules for modular tissue engineering (MTE). In this article, we present the results of optimizing the PLGA MS manufacturing conditions via oil-in-water emulsification. Due to such a technique, MS with the required size, size distribution and properties suitable for cell culturing can be obtained. Three parameters of the oil-in-water emulsification were examined: the stirring speed of a water phase during MS manufacturing, the surfactant concentration, i.e. poly(vinyl alcohol) (PVA) in a water phase and concentration of PLGA in dichloromethane (DCM) as an oil phase. The results proved that the 7.5% PLGA concentration in DCM solution as an oil phase, the 0.5-2% concentration of PVA solution as a water phase and the stirring speed of water phase of 1000 rpm provided MS with the 160 μm mean diameter, which is suitable for cell culture. Moreover, the developed sieving and cleaning procedures were efficient to collect MS with the mean diameter of 280 μm, the more coherent size distribution and the ability to sink in the cell culture medium. The presence on the bottom of cell culture wells is crucial for MTE.
Źródło:: Engineering of Biomaterials; 2020, 23, 156; 2-9
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

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