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Wyszukujesz frazę "cell differentiation" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Elektroprzędzone membrany kompozytowe stymulują mineralizację w hodowlach komórek kostnych
Composite electrospun membranes stimulate mineralization in bone cell culture
Autorzy:
Rajzer, I.
Menaszek, E.
Powiązania:
https://bibliotekanauki.pl/articles/284107.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
podłoża dla inżynierii tkankowej
elektroprzędzone membrany
mineralizacja
różnicowanie komórek
scaffolds for tissue engineering
electrospun membranes
mineralization
cell differentiation
Opis:
Biodegradowalne nanowłókniste membrany poddano badaniom in vitro, pozwalającym na ocenę stopnia różnicowania się i mineralizacji komórek kostnych w obecności potencjalnych podłoży tkankowych. Przedstawione badania oceniają wpływ mikrostruktury i składu chemicznego wytworzonych podłoży na przyczepność, proliferację i morfologię osteoblastów (NHOst). Badania procesu mineralizacji i aktywności ALP pozwoliły na ocenę procesu różnicowania się komórek.
A biodegradable nanofibrous nonwoven membranes were analyzed in vitro as potential scaffolds for differentiation and mineralization of bone cells. In this study we investigate the effects of electrospun membranes microstructure and chemical composition on attachment, proliferation, and morphology of human NHOst osteoblasts. Mineralization process and ALP activity were studied to estimate the cells differentiation.
Źródło:
Engineering of Biomaterials; 2012, 15, 113; 35-39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models
Autorzy:
Kazimierczak, Paulina
Vivcharenko, Vladyslav
Truszkiewicz, Wiesław
Wójcik, Michał
Przekora, Agata
Powiązania:
https://bibliotekanauki.pl/articles/284277.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone tissue engineering
biocompatibility
osteoconductivity
cell growth
osteogenic differentiation
Opis:
Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.
Źródło:
Engineering of Biomaterials; 2019, 22, 151; 24-29
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Integrin αv signaling influences phenotype and maturation of primary human osteoblasts on alumina surface
Autorzy:
Wróbel, E.
Witkowska-Zimny, M.
Mrówka, P.
Głodkowska-Mrówka, E.
Powiązania:
https://bibliotekanauki.pl/articles/284664.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
integrin
human bone-derived cells
osteogenic differentiation
cell adhesion
biomaterial
Opis:
Due to the growing interest in stem cells application in tissue engineering the better understanding of primary human osteoblasts behavior in vitro, on biomaterial surface, is required. Among other molecules integrins may be taken into account as being involved in these phenomena. Integrins are a family of cell adhesion receptors, which may regulate many cellular functions e.g., adhesion, motility, phenotype and cell maturation. The aim of this study was to determine the effect of the biomaterial surfaces and αv integrin signaling pathway on the behavior, phenotype and maturation of human osteoblasts in vitro. Human bone derived cells (HBDCs) obtained from adult femoral bone fragments were cultured on both alumina disks and tissue culture polystyrene (TCPS) dishes. After 7, 14, and 21 days of culture, localization and mRNA expression level of αv integrin subunits and BGLAP (osteocalcin) on polystyrene were analyzed in addition, we treated the cell cultures with monoclonal antibodies against human αv integrin to block its ligand-binding activity, on both alumina and TCPS substrates. We found that the αv integrin was present in focal contacts and cell cytoplasm at subsequent stages of cell maturation and the level of αv integrin mRNA was the highest in mature osteoblasts. Blocking αv integrin transduction pathway caused changes in cell activity and morphology, decreased cells proliferation on TCPS and reduced expression of alkaline phosphatase (ALP) on both materials. The results suggest that αv integrin is involved as an important receptor facilitating osteogenic differentiation.
Źródło:
Engineering of Biomaterials; 2014, 17, 127; 33-39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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