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    Tytuł:
    Microscopic analysis of the nanostructures impact on endothelial cells
    Autorzy:
    Kołodziejczyk, Agnieszka Maria
    Kucińska, Magdalena
    Jakubowska, Aleksandra
    Siatkowska, Małgorzata
    Sokołowska, Paulina
    Kotarba, Sylwia
    Makowski, Krzysztof
    Komorowski, Piotr
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844983.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanostructures
    atomic force microscopy
    transmission electron microscopy
    scanning electron microscopy
    cell morphology
    Opis:
    Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
    Źródło:
    Engineering of Biomaterials; 2020, 23, 154; 2-8
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microscopic analysis of the nanostructures impact on endothelial cells
    Autorzy:
    Kołodziejczyk, Agnieszka Maria
    Kucińska, Magdalena
    Jakubowska, Aleksandra
    Siatkowska, Małgorzata
    Sokołowska, Paulina
    Kotarba, Sylwia
    Makowski, Krzysztof
    Komorowski, Piotr
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/970974.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanostructures
    atomic force microscopy
    transmission electron microscopy
    scanning electron microscopy
    cell morphology
    Opis:
    Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
    Źródło:
    Engineering of Biomaterials; 2020, 23, 154; 2-8
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cartilage tissue examination using atomic force microscopy
    Autorzy:
    Paluch, Jarosław
    Markowski, Jarosław
    Pilch, Jan
    Smółka, Wojciech
    Jasik, Krzysztof Piotr
    Kilian, Filip
    Likus, Wirginia
    Bajor, Grzegorz
    Chrobak, Dariusz
    Glowka, Karsten
    Starczewska, Oliwia
    Powiązania:
    https://bibliotekanauki.pl/articles/27324036.pdf
    Data publikacji:
    2022
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    atomic force microscopy
    cartilage
    biopolymers
    chondrocytes
    intercellular matrix
    mikroskop
    biopolimery
    tkanka
    Opis:
    Life sciences, a field closely intertwined with human biology and physiology, employ various research methods, including morphology studies and quantitative analysis through non-destructive techniques. Biological specimens often consist of three-phase structures, characterized by the presence of gas, liquid, and solid components. This becomes crucial when the chosen research methodology requires the removal of water from samples or their transfer to a cryostat. In the current research, mechanical and topographical examination of cartilage was performed. The materials were generously provided by the Department of Anatomy at the Medical University of Silesia, thereby eliminating any concerns regarding their origin or ethical use for scientific purposes. Our research methodology involved the application of atomic force microscopy (AFM), which minimally disrupts the internal equilibrium among the aforementioned phases. Cartilage, recognized as a ‘universal support material’ in animals, proves to be highly amenable to AFM research, enabling the surface scanning of the examined material. The quantitative results obtained facilitate an assessment of the internal structure and differentiation of cartilage based on its anatomical location (e.g., joints or ears). Direct images acquired during the examination offer insights into the internal structure of cartilage tissue, revealing morphological disparities and variations in intercellular spaces. The scans obtained during the measurements have unveiled substantial distinctions, particularly in the intercellular ‘essence’, characterized by granularities with a diameter of approximately 0.5 μm in ear cartilage and structural elements in articular cartilage measuring about 0.05 μm. Thus, AFM can be a valuable cognitive tool for observing biological samples in the biological sciences, particularly in medicine (e.g. clinical science).
    Źródło:
    Engineering of Biomaterials; 2022, 25, 167; 17--23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
    Autorzy:
    Komorowski, Piotr
    Kołodziejczyk, Agnieszka
    Makowski, Krzysztof
    Kotarba, Sylwia
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844871.pdf
    Data publikacji:
    2021
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bacterial exopolysaccharides
    dextran- -based “microcarriers”
    scanning electron microscopy
    atomic force microscopy
    roughness parameters
    three-dimensional cell culture
    Opis:
    The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
    Źródło:
    Engineering of Biomaterials; 2021, 24, 159; 18-23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Właściwości powierzchniowe matryc otrzymanych z poli(L-laktydo-ko-glikolidu) z rysperydonem i ich zmiany po dwóch tygodniach degradacji
    Surface properties of poly(L-lactide-co-glycolide) matrices with risperidone and their changes after two weeks of degradation
    Autorzy:
    Turek, A.
    Jelonek, K.
    Wójcik, A.
    Dzierżewicz, Z.
    Kasperczyk, J.
    Dobrzyński, P.
    Marcinkowski, A.
    Trzebicka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/285548.pdf
    Data publikacji:
    2010
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    rysperydon
    nośniki leków
    matryce polimerowe
    degradacja
    poli(L-laktyd-ko-glikolid)
    mikroskopia sił atomowych
    risperidone
    drug carries
    polymeric matrices
    degradation
    poly(L-lactide-co-glycolide)
    atomic force microscopy
    Opis:
    W celu optymalizacji leczenia schizofrenii proponowanych jest wiele rozwiązań. Do jednego z nich należy zastosowanie biodegradowalnych, implantowanych systemów uwalniających leki. W niniejszej pracy badano za pomocą mikroskopii sił atomowych zmiany powierzchni matryc otrzymanych z poli(L-laktydo-koglikolidu) (PLAGA) i rysperydonu przed i po dwóch tygodniach degradacji. Analizowano także wpływ degradacji na zmiany ubytku masy matryc polimerowych. Wyniki wskazują na duże możliwości matryc PLAGA do inkorporowania i pułapkowania rysperydonu. Po dwóch tygodniach stwierdzono stabilność matryc (nie uległy one gwałtownej i niekontrolowanej degradacji). Matryce otrzymane z PLAGA posiadają właściwości powierzchniowe odpowiednie dla zastosowania jako biodegradowalne systemy o kontrolowanym uwalnianiu rysperydonu.
    Various solutions are proposed to optimize the therapy of schizophrenia. One of them is the application of biodegradable implantable drug delivery systems. In this work, surface changes of matrices composed of poly(L-lactide-co-glycolide) (PLAGA) and risperidone before and after two weeks of degradation were determined by atomic force microscopy. The influence of degradation on weight loss of matrices was also observed. The results suggest that PLAGA matrices present great potential for incorporation and trapping of risperidone. After two weeks of the studies, the matrices were stable and were not subjected to rapid and uncontrolled degradation. PLAGA matrices have surface properties useful for designing of biodegradable system of controlled risperidone release.
    Źródło:
    Engineering of Biomaterials; 2010, 13, no. 96-98; 117-120
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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