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Wyszukujesz frazę "Zarzeczny, Dawid" wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Monitoring vital functions of A-375 melanoma cell cultures via thin-film nickel capacitors
Autorzy:
Wilczyńska, Aleksandra
Kociubiński, Andrzej
Zarzeczny, Dawid
Szypulski, Maciej
Pigoń, Dominika
Małecka-Massalska, Teresa
Prendecka-Wróbel, Monika
Powiązania:
https://bibliotekanauki.pl/articles/1844972.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
ECIS
nickel
thin layers
melanoma
magnetron sputtering
Opis:
This article deals in the constantly developing branch of microelectronic devices used in various fields of medicine, i.e. diagnostics and treatment of previously incurable human diseases. A method for assessing and monitoring the vital functions of living cells by measuring cellular impedance in real-time using the ECIS® system and a commercial culture substrate is presented. The goal was to develop a substrate significantly less expensive than a commercial substrate that would be suitable for multiple uses and compatible with the ECIS® measurement station. Moreover, thanks to the use of a material with electrochemical properties other than the biocompatible material (gold or platinum) it is possible to observe the cells behavior with regard to the toxic agent. For this purpose, a culture substrate with nickel comb capacitors was used. To make the electrodes, a thin metal layer was sputtered on polycarbonate plates in the magnetron sputtering process. Prior to the next stages, technological masks were designed so as to fit in the ECIS® measuring station. Subsequently, the microelectronic processes of photolithography and etching the metal layer were performed. Finally, the wells were glued onto the culture medium with a biocompatible adhesive. The completed substrates were transferred to the Department of Human Physiology, Medical University of Lublin, for the culture test on A-375 human melanoma cells. The results of the experiment determined the usefulness of the device for monitoring cell culture vital functions by means of impedance measurement.
Źródło:
Engineering of Biomaterials; 2020, 23, 157; 10-14
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface modifications of biomaterial with different cold plasma reactors to improve cell adhesion
Autorzy:
Terebun, Piotr
Kwiatkowski, Michał
Zarzeczny, Dawid
Pawłat, Joanna
Wójcik, Michał
Trzaskowska, Marta
Kazimierczak, Paulina
Przekora, Agata
Powiązania:
https://bibliotekanauki.pl/articles/2172008.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
curdlan
agarose
gliding arc discharge reactor
dielectric barrier discharge plasma jet
dielectric barrier discharge surface reactor
surface modification
Opis:
There is a growing trend in the engineering of biomaterials, focusing on surface modifications of biomaterials to improve their mechanical strength, corrosion resistance, and biological properties. Cold plasma treatment may improve biological properties of biomaterials for biomedical applications by enhancing their integration with host tissue. This study investigated the influence of different cold plasma treatments on the surface properties of a polysaccharides- -based biomaterial to improve cell adhesion to its surface. The samples were subjected to plasma treatment using three different reactors operating at atmospheric pressure: gliding arc discharge (GAD) reactor, dielectric barrier discharge (DBD) plasma jet, and DBD surface reactor. Next, surface chemistry of the biomaterial after plasma treatment was determined by ATR-FTIR analysis. Furthermore, a cell adhesion assay on the samples was carried out using normal human skin fibroblasts (BJ cell line). The attenuated total reflection Fourier transform infrared analysis (ATR- -FTIR) showed that new potential functional groups could be formed on the material surface after plasma treatment. However, plasma treatment of the samples did not enhance cell adhesion to the surface of the polysaccharides-based biomaterial. Thus, the obtained results indicate that plasma treatment using GAD reactor, DBD plasma jet, and DBD surface reactor was not effective for surface modification and cell responses.
Źródło:
Engineering of Biomaterials; 2022, 25, 165; 2--6
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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