Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "Escherichia coli" wg kryterium: Temat


Wyświetlanie 1-7 z 7
Tytuł:
Antibiotic Susceptibility Profiling of Bacterial Biodiversity Associated with Urinary Tract Infections
Autorzy:
Prasad, Bhairav
Arora, Priya
Powiązania:
https://bibliotekanauki.pl/articles/1193375.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiotics
Escherichia coli
Klebsiella
Pseudomonas
Sensitivity profiling
Staphylococcus
Urinary system
Urinary tract infections
Opis:
Urinary tract infections (UTI’s) are a bacterial infection that affects any part of the urinary tract. Urinary tract infection is one of the major diseases that affect people of all age groups and sexes and can be separated into asymptomatic and symptomatic. The normal urinary tract is sterile but gets infected with the normal flora and opportunistic pathogens. Urinary tract infections (UTIs) are a serious health problem affecting millions of people each year. This is the second most common type of infection in the body. In the present study 20 morphologically different bacterial isolates were recovered from 50 different urine samples from UTI’s patient. The prevalence of the isolates was varied with E. coli (40%), followed by Staphylococcus spp., Pseudomonas spp. and Klebsiella spp. each of (20%) respectively. All the isolates of different bacteria were tested for antibiotic sensitivity patterns against common antibiotics viz. Ampicillin, Tetracycline, Chloramphenicol, Vancomycin, Nitrofurontione and Ciprofloxacin with potencies of 30 μg each. The sensitive pattern of the isolates towards antibiotic Nitrofurontoine was (75%) followed by Tetracycline (55%) and Ciprofloxacin (45%). Intermediate activity was reported against Ampicillin (45%), Chloramphenicol and vancomycin (40%). Resistant pattern shown against vancomycin (60%), Chloramphenicol (45%), while both tetracycline and Ciprofloxacin shown (30%) against the UTI’s isolates. Therefore, drugs like Nitrofurontoine, Tetracycline and Ciprofloxacin are the most effective choices against the common UTI’s isolates.
Źródło:
World Scientific News; 2021, 155; 129-139
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The expression level of a recombinant lipase predicted in silico by different codon optimization algorithms
Autorzy:
Bello, Adebayo J.
Igwo-Ezikpe, Miriam
Toye, Emuejevoke T.
Okpuzor, Joy
Powiązania:
https://bibliotekanauki.pl/articles/1046552.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
COOL
Codon optimization
Cohnella
Escherichia coli
ExpOptimizer
JCat
algorithm
in silico
lipase
presyndocon
protein expression
Opis:
Optimizing codons of gene of interest has offered a value-added way to increase heterologous expression of proteins. Different algorithms have been designed to achieve this purpose. These algorithms make use of parameters such as codon Adaptation index (CAI), codon context (CC), percentage guanine-cytosine (GC) content and RNA instability for predicting optimal codons responsible for increased protein expression. Lipase 3646 is an enzyme with great potential for industrial applications. This enzymes has been described to possess thermostable properties with relative stability in high alkaline pH and at different concentrations of organic solvents and inhibitors. This research therefore used JCat, Codon optimization online (COOL), presyndocon and ExpOptimizer algorithms to predict expression level of lipase 3646 enzyme in silico by optimizing its gene coding sequence. The results showed that there were variations in the CAI generated by the algorithms for the same 3646 DNA sequence which suggests that each algorithm is specific for its own generated CAI. COOL algorithm prediction based on other parameters showed good results for potential expression of the lipase. Thus, we recommend COOL algorithm for codon optimization of the lipase 3646 gene for industrial applications.
Źródło:
World Scientific News; 2019, 137; 31-41
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modeling and analysis of batch extraction process for efficient removal of cadmium and pathogens from aqueous solution using modified plantain peel biochar
Autorzy:
Nworie, F. S.
Oti, W. O.
Nwali, U.
Powiązania:
https://bibliotekanauki.pl/articles/1193435.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aspergillus
Biochar
Candida
Escherichia coli
Pseudomonas aeuroginosa
Staphylococcus aureus
cadmium (II)
equilibrium studies
mass transfer
pathogens
Opis:
Consumption of water contaminated with pathogens and heavy metals is responsible for the demise of millions of people around the globe. To find a low cost, facile and benign route for developing a biosorbent capable of handling the challenges associated with unsafe drinking water, the study report the preparation, activation, adsorption and removal of cadmium and pathogenic organisms from water samples. Modified plantain peel biochar (MPPB) was characterized using Braunauer-Emmett-Teller (BET) surface area and X-Ray diffraction (XRD). Surface area and pore size of the MPPB determined from BET surface area plot are 8.79 cc/g and 16.69 m2/g respectively with XRD crystallite size of 14.56 nm. The maximum adsorption capacity was observed to be 366.300 mmolkg-1. Equilibrium isotherm indicated the coefficient of determination to increase from Jovanovich →Elovic→Temkin→Freundlich→Langmuir model. Surface interaction and mass transfer kinetics shows a mixture of intra-particle diffusion and mass transfer process. The adsorption kinetics and surface interaction models coefficient of determination increased from Bangham pore diffusion →mass transfer model→Liquid film diffusion→pseudo first order→Dunwald-Wagner→Elovic→Pseudo-second order→Weber and Moris model. The adsorption indicated a chemisorption process with rate limitimg step likely to involve electron pair donor-acceptor interactions, hydrogen bonding and dipole dipole interactions. Antimicrobial evaluation of MPPB showed that it is a potential antimicrobial agent against common micro-organisms.
Źródło:
World Scientific News; 2021, 157; 1-24
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enteropathogens survivor load in digestate from Two-phase mesophilic anaerobic digestion and Validation of hygienization regime
Autorzy:
Muhondwa, Jacob P.
Powiązania:
https://bibliotekanauki.pl/articles/1075626.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Biotreatment Facility
Contamination
Enteropathogens
Escherichia coli
Hygienization
Mesophilic Anaerobic Digestion
Pathogens
Salmonella Senftenberg
Solanum tuberosum
biowaste
Opis:
The survival of potentially infectious enteropathogens in digestate from two-phase mesophilic anaerobic digestion (Two-phase MAD) technique raises concern on handling and application of the resulting digestate as far as hunan health and environment safety is concerned. In this research, temperature validation and hygienization potential of Two-phase MAD operated as a completely stirred-tank reactor with semi-continuous feeding was investigated. High enteropathogens survival up to 6.4 log E. coli and 6.1 log Salmonella Senftenberg was observed in digestate resulted from anaerobic digestion of potato peels artificially spiked with 8.7 log Salmonella Senftenberg and 8.8log E. coli simulating extra high pathogens contamination in biowaste used as feedstock. Even though an average decrease in pH by 2.3 units from the original value of 6.5 observed in hydrolysis reactor created unfavorable environment on the survival of both E. coli and Salmonella Senftenberg causing a 3.6 log and 3.8 log reduction, yet the alkaline condition in the methanation reactor pH 7.7 boosted the surviving rate by 1.3 log in E. coli and 1.2 log in Salmonella Senftenberg confirming the inefficiency of Two-phase MAD set-up at providing a completely hygienized digestate. This renders the biosafety of the resulting final digestate questionable and limit the possibility of farmapplication for nutrients recycling due to the involving environmental threat and health risks in case the digestate is to be used. Temperature-time combination at 65 °C for 30min was confirmed sufficient at completely elimination of E. coli and effecting >1 log cycle die-off equivalent to 90% die-off in Salmonella Senftenberg with z-Value of 11 ºC validating the temperature-time regime of 70 ºC for 60min proposed in European Commissions Regulation (EC) No. 208/2006.
Źródło:
World Scientific News; 2019, 125; 94-113
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On the biological response of austenitic stainless steels after electrochemical -EP and MEP- polishing
Autorzy:
Hryniewicz, Tadeusz
Lewicka-Rataj, Katarzyna
Rokosz, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1179275.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
austenitic stainless steels
bacteria Escherichia coli
electrochemical polishing
electropolishing EP
magnetoelectropolishing MEP
statistical approach
water contamination
Opis:
The austenitic cold-rolled AISI 316L stainless steel was used for the studies. Corrosion resistance measurements were performed on the samples after three types of treatments: abrasive finishing (MP), standard electropolishing (EP), and magnetoelectropolishing (MEP). They were carried out in Ringer's solution at a room temperature, indicating a considerable difference in the breaking potential Epit values, dependent on surface treatment. Two groups of samples, those after EP and MEP, were submerged in broth culture wild strain of Escherichia coli from contaminated river water. Images of the steel samples submerged for 2 and 4 hours are displayed in the paper. A statistical approach has been performed. In each case the number of bacteria deposited on the MEP steel samples was higher in comparison with that one noticed on EP samples, increasing much faster with time on the MEP ones.
Źródło:
World Scientific News; 2017, 80; 284-296
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel alkoxy (1,2,3-triazole) benzaldehyde hybrids as antimicrobial agents, SAR studies
Autorzy:
Wilson, Christian
Safi, Shahrukh Khan
Dhamsaniya, Ashish
Trivedi, Prachi
Chhatbar, Pratiksha
Shah, Anamik
Powiązania:
https://bibliotekanauki.pl/articles/1062812.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Acinetobacter baumannii
Candida albicans
Cryptococcus neoformans
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Vanilla planifolia
antimicrobial evaluation
benzaldehyde hybrids
triazole
Opis:
A novel series of 2-alkoxy (1,2,3-triazole) benzaldehyde hybrids composed of Vanillin are synthesized for antimicrobial evaluation. Of antimicrobial strains tested, consisted of Staphylococcus aureus ATCC 43300 was treated as gram positive bacteria strain and Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Acinetobacter baumannii ATCC 19606 Pseudomonas aeruginosa ATCC 27853 were evaluated as gram-positive bacteria Candida albicans ATCC 90028 (yeast) Cryptococcus neoformans var. grubii H99 ATCC 208821 yeast.
Źródło:
World Scientific News; 2019, 128, 1; 1-70
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning of a Rift Valley Fever fusion gene in a plant virus derived replicon vector
Autorzy:
Omosimua, Rebecca Oziohu
Iyappan, Gowtham
Obembe, Olawole
Ogunkanmi, Adebayo
Sathishkumar, Ramalingam
Powiązania:
https://bibliotekanauki.pl/articles/1193478.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
DNA Cloning
Escherichia coli DH5α cells
PBYR2e3K2Mc-GFP
PBYR2e3K2Mc-RVFV
RVF disease
RVFV fusion gene
bean yellow dwarf derived vector
emerging disease
geminivirus vector
zoonotic disease
Opis:
Rift Valley fever (RVF) disease is an emerging viral zoonotic disease caused by Rift Valley fever virus (RVFV). RVF disease is seemingly becoming more severe in endemic populations and in areas of new outbreak. RVF disease is listed by WHO as requiring urgent research and development attention. There is no therapeutics or licensed vaccine for human use in the case of an eventual outbreak. There is a need to clone RVFV genes in cloning vectors which may be useful to produce antigens in plant cells or may be tested directly as DNA vaccines. A synthesized Rift Valley fever virus fusion gene was cloned in a bean yellow dwarf virus derived replicon vector; PBYR2e3K2Mc-GFP by removing and replacing the GFP gene. The cloned PBYR2e3K2Mc-RVFV fusion gene was confirmed by PCR, restriction digestion and DNA sequencing. This cloned RVFV fusion gene in plant virus vector can be used for subsequent protein expression in plants cells or used directly tested as DNA vaccines in future studies.
Źródło:
World Scientific News; 2021, 158; 159-172
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

    Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies