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    Wyświetlanie 1-9 z 9
    Tytuł:
    Histopathological changes in gold fish (Carassius auratus (Linnaeus, 1758)) infected by Aeromonas hydrophila bacteria with various densities
    Autorzy:
    Rosidah, Rosidah
    Yunita, Maria Dewi
    Nurruhwati, Isni
    Rizal, Achmad
    Powiązania:
    https://bibliotekanauki.pl/articles/1031492.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Carassius auratus
    clinical symptoms
    histopathology
    Opis:
    The purpose of this study was to distinguish histopathological changes in carp fish (Carassius auratus) infected by Aeromonas hydrophila with different densities. Five treatments were used in the survey for this research method. The treatments used were bacterial infusion of A. hydrophila in the test fish through intramuscular injection with various densities, namely: A. without infection, B: 105 CFU/mL, C: 106 CFU/mL, D: 107 CFU/mL, and E: 108 CFU/mL. The parameters observed were histopathological changes in gill organs, kidneys and hearts of carp fish (gold fish). Data from histopathological observations were analyzed descriptively. The results present gill organs, liver and kidneys of carp fish infected with the bacteria A. hydrophila at various densities experiencing histopathological changes in the form of hemorrhages, necrosis and congestion and hyperplasia of the renal tubules. Other changes that occur were in the kidney organ experiencing a melanomacrophage center (MC). Tubules were coated with connective tissue and lymphocyte infiltration. The most severe organ damage that occurs in carp fish infected with the bacteria A. hydrophila at a density of 108 CFU/mL (treatment E) is shown by a low survival of 5%.
    Źródło:
    World Scientific News; 2020, 142; 150-168
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effectiveness of Bacillus sp. to increase the body resistance of common carp (Cyprinus carpio Linnaeus, 1758) against the attack of Aeromonas hydrophila
    Autorzy:
    Insanie, Zahara Pujie
    Mulyani, Yuniar
    Handaka, Asep Agus
    Rosidah, Rosidah
    Powiązania:
    https://bibliotekanauki.pl/articles/1062804.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Bacillus sp
    Body resistance
    Common carp
    Cyprinus carpio
    Opis:
    This study aims to determine the best density of Bacillus sp. which is given through feed to increase the body resistance of carp that is infected by pathogenic bacteria, Aeromonas hydrophila. The research was conducted at the Aquaculture Laboratory and Laboratory of Microbiology and Biotechnology at FPIK UNPAD. This study used an experimental method with a completely randomized design consisting of 4 treatments and 3 replications, namely treatment A (control), treatment B (Bacillus sp. 106 cfu/mL feed), treatment C (Bacillus sp. 108 cfu/mL feed) and treatment D (Bacillus sp. 1010 cfu/mL feed) for two weeks later in Aeromonas hydrophila infection with an intramuscular density of 108 cfu/mL for seven days. Observation parameters were clinical symptoms, fish response to feed, fish response to shock and survival. The results of this study showed that treatment C (Bacillus sp. 108 cfu/mL feed) resulted in 89% survival and reduced clinical symptoms.
    Źródło:
    World Scientific News; 2019, 133; 263-274
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Growth characteristics and antagonistic potential of Bacillus cereus and Bacillus zhangzhouensis against pathogenic bacteria Aeromonas hydrophila in vitro
    Autorzy:
    Sofandi, Adrianti Nur Fitria
    Mulyani, Yuniar
    Rochima, Emma
    Rosidah, Rosidah
    Powiązania:
    https://bibliotekanauki.pl/articles/1193481.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Bacillus cereus
    Bacillus zhangzhouensis
    antimicrobial activity
    pathogenic bacteria
    Opis:
    This study aims to examine the growth characteristics of B. cereus and B. zhangzhouensis and to see their potential in preventing the growth of Aeromonas hydrophila in vitro. This research was conducted in June 2020 - May 2021 at the Laboratory of Microbiology and Biotechnology, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The method used is the exploratory method and the data obtained were analyzed descriptively. The growth curve was performed using the turbidimetric method. The inhibition test used the disc diffusion method with 5 treatments, chloramphenicol antibiotics (4000 ppm), distilled water, bacterial culture, 100% supernatant and 50% supernatant. The curve results showed that the growth of B. cereus and B. zhangzhouensis was slow. The stationary phase of B. cereus occurred at 40-72 hours, while in B. zhangzhouensis the stationary phase occurred at 48-72 hours. The results of the inhibition test showed that the antimicrobial activity of B. cereus and B. zhangzhouensis was weak with a value range of 0.71 - 1.6mm, besides that the results of the inhibition zone produced by these two bacteria have different inhibitory abilities in each treatment.
    Źródło:
    World Scientific News; 2021, 158; 187-200
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effectivity of Solution Mangosteen Rind (Garciana mangostana) as Medicine for Black Tilapia Juvenile (Oreochromis niloticus Bleeker) when Infected by Aeromonas hydrophila
    Autorzy:
    Lili, Walim
    Gumilar, Resvi
    Nurhayati, Atikah
    Rosidah, Rosidah
    Powiązania:
    https://bibliotekanauki.pl/articles/1062740.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Garciana mangostana
    Oreochromis niloticus
    Solution mangosteen rind
    black tilapia
    medicine
    Opis:
    Many tropical plants have interesting biological activities with potential therapeutic applications. Garcinia mangostana Linn. (GML) belongs to the family of Guttiferae and is named “the queen of fruits”. It is cultivated in the tropical rainforest of some Southeast Asian nations like Indonesia, Malaysia, Vietnam, Philippines, and Thailand. This research aims to determine the concentration of solution mangosteen rind (Garciana mangostana) which is the most effective to healing of black tilapia juvenile (Oreochromis niloticus Bleeker) when infected by A. hydrophila bacteria to produce the highest survival of black tilapia juvenile. This research used experimental method and experimental design by including a complete randomized design (CRD) with five treatments and three replications. The used treatment was soaking black tilapia juvenile for 24 hours in mangosteen rind. The sample infected by A. hydrophila bacteria as much as 0.1 mL/fish at concentration of 108 cfu/mL with intramuscular injection method was used. The concentrations of mangosteen rind solution were 0, 500, 600, 700, and 800 ppm. The parameters observed were clinical symtoms, fish response to feed, fish response to shock, recovery process, and water quality. Data of the clinical symtoms, response fish to feed, response fish to shock, recovery process and water quality were analysed descriptively, while the survival rate data of black tilapia juvenile were analysed using F test at 95% confidence level and continued with Duncan test. The results showed, the use of mangosteen rind solution with 600 ppm concentration was effective to treat tilapia juvenile which infected by A. hydrophila bacteria resulted in the fastest recovery on the third day and the highest survival rate of 91.76.
    Źródło:
    World Scientific News; 2019, 133; 56-70
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening of phytochemical profile and antibacterial activity of various solvent extracts of marine algae Sargassum swartzii
    Autorzy:
    Sujatha, Ravi
    Siva, Durairaj
    Nawas, P. Mohideen Askar
    Powiązania:
    https://bibliotekanauki.pl/articles/1076470.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Antibacterial activity
    Pseudomonas aeruginosa
    Sargassum swartzii
    Total flavonoid
    Total phenol
    Opis:
    The present study investigates phytochemical screening and antibacterial efficacy of various solvent extracts of marine algae such as Sargassum swartzii against some selected human and fish pathogenic bacteria. The brown seaweed Sargassum swartzii was collected from Kudankulam, Tirunelveli district, Tamil Nadu, India. Soxhlet extraction method was used to get the extracts of different solvents like aqueous, ethanol, methanol, acetone and were tested for their qualitative phytochemical screening using common standard tests. Quantitative phytochemical analysis such as total phenolic content and total flavonoid content of S. swartzii and antibacterial activity against human pathogens viz., Escherichia coli, Pseudomonas aeruginosa and Stephylococcus aureus and fish pathogens viz., Aeromonas hydrophila and Vibrio vulnificus bacteria using disc diffusion methods. Preliminary phytochemical screening of sixteen different chemical compounds were carried out. The maximum biochemical compounds were present in the ethanol extract and the minimum compounds were present in aqueous extract. The ethanol extract was significantly higher in phenolic content (15.35 ±2.61 mg of GAE/g) and the methanol extract was significantly higher in flavonoides content (26.92±7.14 mg of QE/g). In human pathogen the highest antibacterial activity was present in Pseudomonas aeruginosa (18.00±0.00mm) and the fish pathogen was significantly higher in Aeromonas hydrophila. The present study showed that the ethanol extract of marine brown seaweed Sargassum swartzii contains bio active constituents with highest antibacterial activity against human and fish pathogen.
    Źródło:
    World Scientific News; 2019, 115; 27-40
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The performance of Koi (Cyprinus carpio L.) in a circular current container tested against the Aeromonas hydrophila bacteria
    Autorzy:
    Suryadi, Ibnu Bangkit Bioshina
    Yusa, Regan Hanifelian
    Roffi, Grandiosa H.
    Zahidah, Zahidah
    Powiązania:
    https://bibliotekanauki.pl/articles/1193489.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Currents
    Cyprinus carpio
    Koi Fish Fingerlings
    Round Container
    System Immune
    Ventury System
    Opis:
    This research aims to determine the best currents speed and utilization of venturi to increasing survival, growth of Cyprinus carpio L. and reducing the infection rate of A. hydrophila bacteria. fingerlings. This research used an experimental method completely randomized design (CRD) with four treatments and four replications. The treatments used were the use of currents and venturi on the maintenance media with currents velocities of 0 m/s (A), 0.06 m/s (B), 0.1 m/s (C), and 0.14 m/s (D). Maintenance of the test fish for 40 days then followed by a challenge test for 14 days. Koi fish fingerlings tested to use A. hydrophila with a density of 108 CF /ml. The parameters observed included white blood cells, red blood cells, clinical symptoms, survival, daily growth rate, feed conversion ratio and water quality. The results showed that the use of currents and venturi with a flow rate of 0.14 m/s was effective in improving the performance of the koi fish's immune system and reducing the infection rate of A. hydrophila bacteria. During the flow administration during the 40 days maintenance period, the white blood cell count increased by 20.51% from a value of 9.19 × 104 cells/mm3 to 11.56 × 104 cells/mm3. Red blood cells increased by 40.65% from 1.60 × 106 cells/mm3 to 2.69 × 106 cells/mm3. Currents treatment with a speed of 0.14 m/s produced the highest survival rate after challenged with a value of 86.7%. Also this treatment was effective in preventing infection of A. hydrophila bacteria, it can be seen from the macroscopic clinical symptoms that appear less than the treatment current. The best results of daily growth rate and feed conversion ratio provided in currents treatment with a speed 0.1 m/s.
    Źródło:
    World Scientific News; 2021, 159; 1-19
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effectiveness of potassium diformate addition to feed to improve immune system of Pangasius (Pangasianodon hypophthalmus) that challenged by Aeromonas hydrophila
    Autorzy:
    Yustiati, Ayi
    Kundari, Della Fauzia
    Suryana, A. A. Handaka
    Suryadi, Ibnu Bangkit Bioshina
    Powiązania:
    https://bibliotekanauki.pl/articles/1058052.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Pangasianodon hypophthalmus
    Pangasius
    potassium diformate
    red blood cells
    survival rate
    white blood cells
    Opis:
    This research was conducted from October 2018 until April 2019 in the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This study aims to determine the optimum potassium diformate dosage which was added to feed to increase the immune status of Pangasianodon hypophthalmus (Sauvage, 1878). The research method used is the experimental Complete Random Design (CRD) with 4 treatments and 3 replications. The treatments are A (control), B (0.3% KDF), C (0.5% KDF), and D (0.7% KDF). Observed parameters are survival rate, gross clinical signs, intestine pH levels, total leukocyte count (white blood cells), total erythrocyte count (red blood cells), and water quality. The results showed that the 0.5% KDF addition in feed is the most effective to improve immune status. This treatment also is the most effective to improve Pangasius immune status after being challenged with A. hydrophila with survival rate of 93.33%, leukocyte count 2.98×106 cells/mm3 and erythrocyte count 1.02×106 cells/mm3. Increased leukocyte count was 65.26% and erythrocyte count - 58.23%.
    Źródło:
    World Scientific News; 2019, 134, 2; 86-100
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Growth Characteristics and Tracing Antagonistic Properties of Bacillus flexus and Bacillus subtilis as Antibacterials to Overcome the Attack of Aeromonas hydrophila Bacteria on Fish
    Autorzy:
    Amatulloh, Zulfahana
    Mulyani, Yuniar
    Dewanti, Lantun Paradhita
    Haetami, Kiki
    Powiązania:
    https://bibliotekanauki.pl/articles/1193472.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Bacillus carboniphilus
    Bacillus flexus
    Bacillus haynesii
    Bacillus subtilis
    Bacillus tequilensis
    Bacillus zhangzhouensis
    Inhibition Zone Test
    fish
    Opis:
    This research was conducted to assess the antibacterial potential of Bacillus flexus and Bacillus subtilis against Aeromonas hydrophila. Bacterial samples came from the stock of the FPIK Unpad Biotechnology Laboratory. This research was conducted in July 2020 - May 2021 in Laboratorium Biotechnology FPIK Padjadjaran University. This research was conducted using qualitative and quantitative descriptive analysis. Bacteria were collected using the purposive sampling method, Observation of the growth curve using the turbidimetric method, and the disc diffusion method for the inhibition zone test with bacterial supernatant treatment with a concentration of 50%, a concentration of 100%, bacterial culture, chloramphenicol antibiotics with a concentration of 4000 ppm as a positive control. The results showed that B. flexus bacteria experienced an exponential phase at 6 to 12 hours, after which it entered a stationary phase at 14 to 72 hours. B. subtilis bacteria experienced an exponential phase at 20 hours. Until 40 hours, then experiencing a stationary phase at 42 hours to 72 hours, and the growth of A. hydrophila bacteria experiences an exponential phase at 6 hours to 28 hours, the stationary phase at 30 to 28 hours 72. Based on the results of the inhibition zone test, it showed the formation of the inhibition zone only in the positive control treatment, while the supernatant and bacterial cultures of B. flexus and B. subtilis didn’t form an inhibition zone.
    Źródło:
    World Scientific News; 2021, 158; 91-104
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hematological Performance and Survival of Common Carp (Cyprinus carpio Linnaeus, 1758) Strain Majalaya Fingerlings Against Temperature Stress
    Autorzy:
    Yustiati, Ayi
    Saefulhadjar, Sadra Muhammad Haydar Ali
    Suryadi, Ibnu Bangkit Bioshina
    Iskandar, Iskandar
    Powiązania:
    https://bibliotekanauki.pl/articles/1031780.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    C. c. haematopterus
    Common carp Strain Majalaya
    Cyprinus carpio
    maintenance temperature
    number of erythrocytes
    number of leukocytes
    survival rate
    Opis:
    The research was conducted at the Aquaculture Laboratory of the Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor. The purpose of this research is to determine the optimum temperature for the maintenance of Common carp strain Majalaya fingerlings based on resistance to temperature stress that is known through their blood profile and survival rate. Common carp strain Majalaya fingerlings originating from Ciparay, West Java. Total body length is 8-12 cm with a minimum weight of 10 g. The method used is experimental with a completely randomized design (CRD) consisting of three treatments and five replications. The fish are kept at 24 ºC (control without heater), 29 ºC and 34 ºC. Each experimental unit contains 10 fishes. The results obtained by the survival rate (SR) of Common carp strain Majalaya which were maintained at a treatment temperature of 24 ºC by 100%, the 29 ºC by 70%, the 34 ºC by only 42% remained on the 21st day. The number of erythrocytes of common carp strain Majalaya fingerlings before the treatment temperature was 2.77 × 106 cells/mm3. The average number of erythrocytes of the 24ºC treatment from 7th, 14th and 21st day were 2.82, 2.82 and 2.81 × 106 cells/mm3; the 29 ºC were 2.93, 2.68 and 2.42 × 106 cells/mm3; the 34 ºC were 3.08, 2.52 and 1.99 × 106 cells/mm3. While the number of leukocytes of common carp strain Majalaya fingerlings before the treatment temperature was 13.61 × 104 cells/mm3. The average number of leukocytes of the 24 ºC treatment from 7th, 14th and 21st day were 13.73, 13.71 and 13.88 × 104 cells/mm3; the 29 ºC were 18.40, 16.46 and 15.76 × 104 cells/mm3; the 34 ºC were 20.78, 18.46 and 16.81 × 104 cells/mm3.
    Źródło:
    World Scientific News; 2020, 150; 92-104
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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