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    Wyświetlanie 1-6 z 6
    Tytuł:
    Isolation of vanconycin resistant Staphylococcus aureus from the wounds of hospital patients in Uyo, Akwa Ibom State, Nigeria
    Autorzy:
    Anosike, I. K.
    Ebana, R. U. B.
    Edet, U. O.
    Umoh, I. S.
    Powiązania:
    https://bibliotekanauki.pl/articles/1076716.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Methicillin
    Staphylococcus aureus
    Vancomycin
    Wounds
    antibiotic resistance
    Opis:
    Staphylococcus aureus is a very important human pathogen that generates a number of human infections. Isolation of vancomycin-resistant Staphylococcus aureus (VRSA) was carried out from wound samples of patients attending University of Uyo Teaching Hospital, Akwa Ibom state, Nigeria. A total of 45 wound samples from 15 patients were collected aseptically in triplicate, using sterile cotton swabs moistened in sterile normal saline. Microbiological analysis and susceptibility to vancomycin, including minimum inhibitory and minimum bactericidal concentrations (MIC and MBC), were carried out using standard methodologies. A total of 15 isolates were obtained and these showed varying MIC and MBC patterns. Out of the 15 S. aureus isolated, only one isolate had an MIC of approximately 4 µg/ml, while twelve isolates gave MIC values that ranged from 15.62 µg/ml to 250 µg/ml. The remaining 2 isolates gave MIC values that were ≥ 500 µg/ml. These 2 isolates exhibited alpha haemolysis on blood agar, unlike the others that were beta haemolytic. The results of the MBC also showed variations amongst the isolates. A total of 10 isolates gave MBC values that ranged from 62.5 µg/ml to 500 µg/ml, while the remaining 5 isolates gave MBC values that were ≥ 500 µg/ml. The high MIC and MBC values obtained showed that vancomycin-resistant Staphylococcus aureus is increasing at an alarming rate, and this accounts for the gradual decline in the effectiveness associated with the use of vancomycin. Given the widespread prevalence of VRSA, there is a need for newer therapeutics that can reverse this surge.
    Źródło:
    World News of Natural Sciences; 2019, 24; 134-144
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibacterial activity of covered paper after storage
    Autorzy:
    Jarosz, Michał
    Sumińska, Patrycja
    Kowalska, Urszula
    Mizielińska, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1112792.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Staphylococcus aureus
    active coatings
    covered paper
    polylysine
    Opis:
    The paper covered with a hydrophobic Topscreen coating was coated with the second layer. The goal of paper covering was to obtain the antimicrobial properties of the external coating. The samples were stored 2 months at 20 ºC. The influence of storage on the antimicrobial properties of the external coating was analyzed. The results of the study showed that paper covered with Topscreen coating did not have an influence on the growth of Staphylococcus aureus cells. The second (external) layer containing 2% polylysine as an active substance decreased the growth of S. aureus. The 2 months storage of the covered paper did not influence the antimicrobial properties of coating with polylysine against S. aureus. It was demonstrated that paper covered with hydrophobic coating had no influence on the growth of E. coli cells as well. In this case the influence of 2 months storage on the antimicrobial properties of the coating with polylysine was observed. In contrast to the results obtained for the samples that were not store, the decrease of the growth of the bacterial cells after 24 h contact with a hydrophobic coating devoid of an active substance was noticed.
    Źródło:
    World News of Natural Sciences; 2018, 17; 141-146
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibacterial activity of (PVP-ZrO2) nanocomposite against pathogenic bacteria
    Autorzy:
    Abdulrazaq, Raghad Abdullatif
    Al-Ramadhan, Zainab A.
    Khalaf, Hadeel H.
    Powiązania:
    https://bibliotekanauki.pl/articles/1112560.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Anti-Bacterial activity
    Antibiotics
    Klebsiella pneumoniae
    Nanocomposities
    PVP
    Staphylococcus aureus
    ZrO2
    Opis:
    The antibacterial activity of a PVP-ZrO2 nanocomposite was investigated against pathogenic bacteria S. aureus and K. pneumoniae after antibacterial sensitivity was determined and one isolate was chosen that showed more antibiotic resistance. Herein, the Co-culture technique was used to calculate percent reduction of bacteria. The results that were obtained in this method show that ZrO2 nanoparticles have inhibitory effect against pathogenic bacteria gram negative bacteria and gram positive bacteria - with reduction of growth reaching 100% to both S. aureus and K. pnumoniae at 5, 10, 15, 20 and 25% ZrO2, compared with control. The resistance patterns of S. aureus and K.pnuemonia isolates show the Moxifloxacin (MXF) is the best antibiotic for both bacteria - with sensitivity at 100%, while resistance to Ceftriaxone (CRO) is at 90% S. aureus, and at 80% K. pnumoniae. The polymer-nanocomposite was prepared by weight percentage wt. % of (PVP) being dissolved in (10) ml of distilled water, with weight percentages 5%, 10%, 15%, 20% and 25% of ZrO2 nanoparticles added.
    Źródło:
    World News of Natural Sciences; 2018, 18, 2; 187-194
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biosynthesis of cobalt oxide (Co3O4) nanoparticles using plant extract of Camellia sinensis (L.) Kuntze and Apium graveolens L. as the antibacterial application
    Autorzy:
    Urabe, Aliyaa A.
    Aziz, Wisam J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1076785.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Apium graveolens
    Camellia sinensis
    Cobalt oxide nanoparticles
    Pseudomonas aeruginosa
    Staphylococcus aureus
    Opis:
    In this work, we prepared cobalt oxide nanoparticles using Celery stalks and green tea leaves extract. The synthesized cobalt-oxide NPs were characterized using X-Ray diffraction. This showed the highest peak and top control (222) at (38.18 degree) with regard to Camellia sinensis extract and (220) at (30.14 degree) for Apium graveolens extract, Field Emission scanning electron microscopy (EF_SEM) at the range of 21-72 nm, revealed the highly uniform shape of particles, while UV-Visible spectroscopy techniques recorded the highest value of the absorptive at 230 nm and the energy band gap to be 3.55 eV for Camellia sinensis and 225 nm and energy band gap 3.85 eV at Apium graveolens, respectively. Our results indicate that the best achievable result in inhibiting bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa comes by way of using Camellia sinensis extract (27-29 mm).
    Źródło:
    World News of Natural Sciences; 2019, 24; 356-364
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia
    Autorzy:
    Geta, Kindu
    Kebede, Ameha
    Chemedissa, Meseret
    Powiązania:
    https://bibliotekanauki.pl/articles/1076786.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibiotics
    Antimicrobial resistance
    B. cereus
    Debre Markos
    E. coli
    Enterobacter
    Fruit juices
    Klebsiella
    Pathogens
    Ps. aeruginosa
    Salmonella
    Shigella
    Staphylococcus aureus
    Opis:
    The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.
    Źródło:
    World News of Natural Sciences; 2019, 24; 365-371
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microbiological safety of fruit juices consumed in cafes and restaurants of Debre Markos town, north western Ethiopia
    Autorzy:
    Geta, Kindu
    Kebede, Ameha
    Chemedissa, Meseret
    Powiązania:
    https://bibliotekanauki.pl/articles/1076778.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aspergillus niger
    Bacillus cereus
    Debre Markos
    Enterobacter
    Enterococcous
    Escherichia coli
    Fruit juice
    Hygiene
    Klebsiella
    Microbial safety
    Penicillium
    Salmonella
    Serratia
    Shigella
    Staphylococcus aureus
    Streptococcus
    Opis:
    Improperly prepared fresh fruit and vegetable juices are recognized as an emerging cause of food borne illnesses. Therefore, this study was aimed at evaluating the microbiological safety of fresh fruit juices marketed in Debre Markos town and their hygienic conditions of preparations. Thirty-six fruit juices samples were collected from 6 cafés and restaurants of Debre Markos town and analyzed for total aerobic viable bacterial count (TAVBC), total staphylococcal count (TSC), aerobic spore forming bacterial count (ASFBC), total coliform count (TCC), fecal coliform count (FCC), yeast and mould count (YMC). The spread plate method was used for the isolation of microorganisms on appropriate selective media. All isolates were characterized following standard methods. Bacterial and fungal species were isolated following standard methods, while questionnaires were distributed to 30 juice makers to obtain preliminary information on their hygienic and safety practices. Results show that the mean TAVBC, ASFBC, TSC, yeast and mould, TCC and FCC of mango were 2.2±0.48×106, 0.13±0.04×105, 0.004×105, 1.1±0.2×106, 0.15±0.05×105, 5.7±3.73×104 and 0.06±0.04×104, cfu/ml, respectively. Moreover, the mean of TAVBC, ASFBC, TSC, YMC, TCC, and FCC of avocado juice were 3.6±0.6×106, 0.08±0.02×105, 0.27±0.07×105, 1.2±0.4×106, 0.02±0.01×105, 6.46±3.7×104, and 0.2±0.1×104 cfu/ml, respectively. The bacterial isolates were identified as S. aureus, E. coli, Klebsiella spp. B. cereus, Enterobacter spp., Enterococcous spp., Streptococcus spp., and Serratia spp., while the identities of the fungal isolates were Fusarium spp., Mucor spp. and S. cerevisiae. The results also showed that the microbial loads of most of the fruit juices were higher than the specifications set for fruit juices sold in the Gulf region and other parts of the world. Most venders obtained fruit from the open market and all juice makers lacked special training in food hygiene and safety. Therefore, regular training and health education on food hygiene and safety is recommended for juice handlers to improve the quality of fresh fruit juices in the study area.
    Źródło:
    World News of Natural Sciences; 2019, 24; 287-298
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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