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    Wyświetlanie 1-8 z 8
    Tytuł:
    The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
    Autorzy:
    Moskwa, B.
    Bień, J.
    Cabaj, W.
    Korinkova, K.
    Koudela, B.
    Kacprzak, E.
    Stefaniak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144348.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    human disease
    serodiagnosis
    diagnosis
    trichinellosis
    ELISA test
    zoonotic disease
    ELISA procedure
    excretory-secretory antigen
    Trichinella spiralis
    human outbreak
    Opis:
    Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 3; 231-238
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Difficulties associated with the diagnosis of mycosis of the oral cavity and throat in chronic lymphocytic leukemia (CLL)
    Autorzy:
    Kaczmarczyk, D.
    Morawiec-Sztandera, A.
    Niedźwiecka, I.
    Kurnatowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143364.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    diagnosis
    mycosis
    oral cavity
    throat
    chronic lymphocytic leukemia
    fungal infection
    disease risk
    fungi
    cancer formation
    neoplastic process
    treatment
    human disease
    disease symptom
    immunodeficiency
    prevalence
    Opis:
    Cases of fungal infections are being encountered more often in clinical practice. The factors associated with a high risk of mycoses include, among others, corticosteroidotherapy, the administration antibiotics with wide spectrum of antibacterial properties, neutropenia, neoplasms. Fungi may play a role in cancer formation, may act as a complication in the course of treatment, and may mimic a neoplastic process by giving a similar clinical picture. In the case of fungal throat infection, patients complain of increased body temperature, a general feeling of weakness, malaise, headache, spontaneous pain intensifying during swallowing, a feeling of an obstacle in the throat or a cough. A physical examination may reveal congestion of the mucosa followed by a unilateral crater ulceration often covered with fat, as well as a thick coating, which is accompanied by foetor ex ore. The submandibular and neck lymph nodes are often greatly enlarged and painful. These symptoms may resemble those associated with the neoplastic process and changes in the course of systemic diseases (agranulocytosis). A correct diagnosis in these cases is necessary for adequate therapy. Chronic lymphocytic leukemia (CLL) is the most common type of leukemia among adults in Europe and North America. It is estimated that in Poland, CLL affects approximately 1,400 people per year. In this paper, a case of 62-years old patient with CLL with fungal infection of oral cavity and throat is presented.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 3; 155-158
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Parasitic diseases and fungal infections - their increasing importance in medicine
    Autorzy:
    Blaszkowska, J.
    Wojcik, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143277.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    fungal infection
    medicine
    parasite
    parasitology
    mycology
    parasitosis
    mycosis
    diagnosis
    intestinal nematode
    nematode
    pathogenesis
    Opis:
    Basing on 43 lectures and reports from the scope of current parasitological and mycological issues presented during the 50th Jubilee Clinical Day of Medical Parasitology (Lodz, 19–20 May 2011), the increasing importance of parasitic diseases and mycoses in medicine was presented. Difficulties in diagnosis and treatment of both imported parasitoses (malaria, intestinal amoebiosis, mansonelliasis) and native parasitoses (toxoplasmosis, toxocariasis, CNS cysticercosis), as well as parasitic invasions coexisting with HIV infection (microsporidiosis) have been emphasized. The possibility of human parasites transmission by vertical route and transfusion has been discussed. The important issue of diagnostic problems in intestinal parasitoses has been addressed, noting the increasing use of immunoenzymatic methods which frequently give false positive results. It was highlighted that coproscopic study is still the reference method for detecting parasitic intestinal infections. The mechanism of the immune reaction induced by intestinal nematodes resulting in, among others, inhibition of the host innate and acquired response was presented. Mycological topics included characteristics of various clinical forms of mycoses (central nervous system, oral cavity and pharynx, paranasal sinuses, nails and skin), still existing problem of antimicrobial susceptibility of fungal strains, diagnostic and therapeutic difficulties of zoonotic mycoses and the importance of environmental factors in pathogenesis of mycosis.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 4; 205-218
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analiza objawów klinicznych i wybranych wskaźników hematologicznych u hospitalizowanych dzieci z zarażeniem Ascaris lumbricoides z rejonu północno-wschodniej Polski
    Analysis of clinical symptoms and selected hematological indices in hospitalized children with Ascaris lumbricoides infection from the northeastern region of Poland
    Autorzy:
    Wasilewska, J.
    Kaczmarski, M.
    Sawicka-Żukowska, M.
    Tomaszewska, B.
    Majewska, A.
    Plewa, K.
    Ołdak, E.
    Dębkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143294.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    ascariasis
    Polska
    child
    clinical symptom
    hematological index
    human disease
    Ascaris lumbricoides
    blood
    helminth
    parasite
    diagnosis
    Opis:
    Ascariasis is the most common soil-transmitted helminth infection in the world. The objective of this study was to analyze the clinical symptoms and selected hematological indices of ascariasis in hospitalized children from the northeastern region of Poland. Patients in the Pediatric Ward hospitalized in the Regional Hospital in Dąbrowa Białostocka in the period of 2005–2007 were included in this retrospective study. The intestinal stage of ascariasis was diagnosed on the basis of positive coprological survey performed using the decantation technique. A total of 938 patients were included in the study, 1801 stool samples were evaluated, and A. lumbricoides-positive tests were obtained from 252 children. Ascaris-positive young children (3 yrs) accounted for 3.0% of all hospitalized children, Ascarispositive preschool-aged children (4–7 yrs) accounted for 8.1% and school-aged children (8–18 yrs) for 15.8%. Seasonal patterns were observed in the prevalence of A. lumbricoides (maximum in August–December). There was no relationship between BMI z-score, hemoglobin levels and prevalence of infection with Ascaris lumbricoides. Significant predictors of intestinal stage ascariasis in a multivariate logistic regression model were: abdominal pain as a reason for hospital admission (OR–2.19; 95%CI 1.62–2.95; p<0.001) and age from 4 to 7 years (OR–2.0; 95%CI 1.41–2.80; p<0.001). The prevalence rate of ascariasis was not higher in the group of patients with atopic diseases (bronchial asthma, allergic rhinitis, atopic dermatitis) and co-existing ascariasis did not affect the eosinophil counts in the peripheral blood. Ascariasis is still a current pediatric clinical problem characterized by non-specific clinical manifestations, which should be taken into consideration in the differential diagnosis of children’s diseases.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 1; 43-51
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Otitis externa: the analysis of relationship between particular signs-symptoms and species and genera of identified microorganisms
    Autorzy:
    Kurnatowski, P.
    Filipiak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143724.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    external otitis
    otitis
    etiological factor
    disease symptom
    patient
    laryngology
    microorganism
    identification
    pathogen
    bacteria
    fungi
    diagnosis
    Opis:
    Objective. To investigate a relationship between the etiological factor of external otitis and occurrence of particular signs/symptoms. Design. A special questionnaire was designed and completed by all patients covering personal details, medical history, results of otolaryngological examination and bacteriological and mycological investigations. Subjects. 249 patients of the Outpatient Department of Laryngology at the Regional Hospital in Bełchatów with symptoms of external otitis. For analysis of relationships between particular signs/symptoms and species/genera of microorganisms, statistical tests were used: χ2 test, χ2 test with Yate’s modification, C−Pearson index, and Fisher exact test for very small samples. Results. There is a statistical dependence between discharge, hearing loss, swelling of skin, scant, dried discharge with fetid odour and bacteria isolated from the external ear canal. Similar dependence exists between pain, hearing loss, no smelly discharge or wet, black plug of fetid odour and fungi. Also there is a statistical dependence between pruritus, red skin and grey, fetid discharge and mixed flora. Conclusions. Some of symptoms and signs are connected with definite etiological factors which is important not only for correct diagnosis but also for institution of appropriate and effective treatment. On the basis of some characteristic symptoms and signs it is possible to make a tentative diagnosis as to the etiological pathogen responsible for external otitis.
    Źródło:
    Wiadomości Parazytologiczne; 2008, 54, 1; 37-41
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A case of Dipylidium caninum infection in a child from the southeastern Poland
    Autorzy:
    Szwaja, B.
    Romański, L.
    Ząbczyk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143353.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Polska
    child
    human infection
    Dipylidium caninum
    zoonosis
    dog
    disease symptom
    tapeworm
    dipylidiasis
    cat
    diagnosis
    treatment
    epidemiology
    Opis:
    Dipylidium caninum is a common intestinal tapeworm of dogs, cats and foxes. However, it occasionally infects also humans. We present a case of D. caninum infection in a 2-year-old child living in the Subcarpathian province. The infection was asymptomatic in the first months. The symptoms of abdominal pains, sleep disorders, loss of appetite, hyperactivity and occasional slimy stools appeared later. Proglottids on the underwear, in water while bathing and mobile proglottids passed with the stool were also observed. Prior to appropriate diagnosis the child was treated with pyrantelum (Pyrantelum) and albendazolum (Zentel). However, proglottids were found again in the stool after a few days. We examined stool samples and perianal smears collected from the child and his family. The stool samples were tested by coproscopic methods. Direct methods (direct preparation in 0.9% sodium chloride and in Lugol’s solution, Kato thick smear) and concentration methods (decantation with distilled water and Faust’s zinc sulphate centrifugal flotation) were used. In the stool samples taken from the child, we observed D. caninum proglottids demonstrating lateral genital pores and many packets of eggs containing from one to a few, mostly 3 to 4 eggs. In the direct preparations in 0.9% sodium chloride and in Lugol’s solution single packets with D. caninum eggs were detected. In decantation preparations many D. caninum egg packets were observed. It has to be reported that the child’s mother was infected with Giardia intestinalis. Dipylidiasis in humans is a rarely encountered infection in Poland and the diagnosis may be difficult. For these reasons we reported clinical case presentation, diagnostics, treatment and epidemiology of D. caninum infection. We have shown that concentration methods such as decantation might be very helpful in the diagnosis of dipylidiasis.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 3; 175-178
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Internet in medical activity
    Autorzy:
    Kacki, E.
    Stempczynska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143221.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Internet
    medicine
    medical activity
    computer network
    disease prevention
    pest prevention
    diagnosis
    treatment
    patient monitoring
    medical education
    hospital administration
    information system
    Opis:
    The paper presents ample opportunities to use the Internet to effectively support medical activities. The presented considerations include prophylaxis, diagnosis and treatment, monitoring the patient during convalescence, medical education and hospital administration elements. The major importance of multimedia software, virtual reality and artificial intelligence algorithms for the effective operation of the telemedicine systems have been accentuated. In the paper the main tasks of telemedical systems connected with the following domains have been presented: 1) prophylaxis – indication of the risks of improper lifestyle and environmental degradation coupled with recommendations on the ways and means of diseases prevention and methods for self-control of health; 2) diagnostics – offering multimedia algorithms of diagnostics procedures; 3) therapy – providing multimedia algorithms of therapeutic procedures and of multimedia information on medicine administration; 4) care during convalescence – constant remote monitoring of the patient’s condition and assurance of quick contact with doctor; 5) medical education – offering attractive presentation of medical knowledge in the diagram form of diagnostic ortherapeutic algorithms and providing simulators creating correct habits in medical procedures; 6) hospital administration – providing comprehensive information about a hospital or clinic functioning for a potential patient and exchange of information concerning patients between hospitals. The paper presents conclusions about conditions of greatest usefulness of telemedicine systems
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 4; 219-222
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiological and clinical picture of parasitic infections in the group of children and adolescents from north-east region of Poland
    Autorzy:
    Żukiewicz, M.
    Kaczmarski, M.
    Topczewska, M.
    Sidor, K.
    Tomaszewska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143366.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic infection
    child
    adolescent
    Polska
    human disease
    epidemiology
    Ascaris lumbricoides
    Toxocara
    Giardia lamblia
    risk factor
    clinical symptom
    Strongyloides stercoralis
    Enterobius vermicularis
    ELISA test
    diagnosis
    Opis:
    The epidemiological and clinical purview of parasite diseases is constantly evolving, mainly due to the changes in human behavior and our environment. The aim of this study was frequency analysis of certain parasite infections, risk factors and clinical symptoms in children and adolescents of north-eastern Poland. 120 children were tested due to the symptoms resembling parasite infection from December 2008 to May 2009. 90 patients (the tested group) aged from 5 months to 18 years were found to be infected with one or more than one of the following parasites: Ascaris lumbricoides, Giardia lamblia, Toxocara spp., Strongyloides stercoralis or Enterobius vermicularis. 30 children with no infections found were the control group. Parasites were found by means of microscope decantation stool tests, RIDA Quick Giardia stool test and ELISA blood test (Toxocara IgG). The patients’ parents filled out the survey concerning the probability factors of infection and the ailments that were the cause of parasitical diagnosis. High percentage of either isolated or co-invasive parasite infections was ascertained in the tested group – 75%. The prevalence of ascariasis was 55.83%, toxocariasis – 16.67%, giardiasis – 12.5%, strongyloidiasis – 5.83% and of enterobiasis – 3.33%. The statistical significance of higher dirty hands–mouth contact frequency and more frequent presence of domestic animals in the tested group than in the control group was ascertained. A highly important correlation between the infection and the occurrence of symptoms from at least 3 various systems was ascertained. The presence of habits involving dirty hands – mouth contact and having domestic animals are significant risk factors of parasite infections. The presence of symptoms from at least 3 systems should compel to parasite infection diagnosis with proper differential diagnosis.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 3; 179-187
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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