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    Wyświetlanie 1-7 z 7
    Tytuł:
    Pozamózgowe inwazje Acanthamoeba spp. u myszy
    EXTRACEREBRAL INFECTIONS OF ACANTHAMOEBA SPP. IN MICE
    Autorzy:
    Mazur, T.
    Jóźwiak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152213.pdf
    Data publikacji:
    1993
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pasozyty zwierzat
    choroby inwazyjne
    Acanthamoeba
    zwierzeta doswiadczalne
    parazytologia
    myszy
    ameby
    inwazja pasozytnicza
    Opis:
    A variety of Acanthamoeba spp. pathogenic strains were isolated from water pools in the area of Poznań. In many cases amoebae invaded in mice, beside central nervous system (CNS) also lungs, kidney, heart, liver, diafragm and eyeballs, causing significant changes in these organs. The presence of amoebae outside of CNS was found mainiy in the early period of infection. Amoebae isolated Crom different organs of dead animals did not exhibit any specificity in relation to the organs in following infections.
    Źródło:
    Wiadomości Parazytologiczne; 1993, 39, 4; 357-366
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wartość testu barwnego w ocenie żywotności cyst Acanthamoeba sp.
    Efficiency of eosin exclusion in estimation of Acanthamoeba cyst viability
    Autorzy:
    Mazur, T.
    Jóźwiak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152555.pdf
    Data publikacji:
    1989
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pierwotniaki
    ameby
    pasozyty
    Acanthamoeba
    cysty
    zywotnosc
    metody oceny
    testy barwne
    ocena przydatnosci
    Opis:
    The eosin colour test estimating the viability of cysts was compared with the method of in vitro excystation on agar substrate (NNE). Single cysts were studied, isolated from populations stored under sterile conditions in water at 4°C for a period ranging from a few days to 16 years. It has been found that the inoculations of single cysts (805 cysts) on the NNE substrate allow a precise determination of the percentage of living cysts. All the cysts stained with eosin, incubated on such a substrate proved to be dead, while the non-staining cysts ex-cistated with varying percentage. Thus, among the non-staining cysts of Acanthamoeba after 2-4 years of storage at 4°C 89.2% excystated while the remaining ones were dead. After 16 years of storage only 29.8% nostaining cysts excystated. Very similar values (89.6 and 28.5% respectively) were obtained when determining the percentage of living cysts with the colour test. The eosin test has proved to be a good method of the estimation of the viability of cysts of Acanthamoeba.
    Źródło:
    Wiadomości Parazytologiczne; 1989, 35, 1; 11-17
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wykorzystanie obecności celulozy w ścianie komórkowej cyst Acanthamoeba do celów diagnostycznych
    Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes
    Autorzy:
    Derba, M.
    Hadasś, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143498.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    celuloza
    choroby pasozytnicze
    sciany komorkowe
    cysty
    Acanthamoeba
    wykorzystanie
    pierwotniaki
    diagnostyka
    pasozyty
    budowa sciany komorkowej
    Opis:
    Species identification within the genus Acanthamoeba is based predominantly on morphological and biochemical features. It is labor-intensive and requires cloning and axenization. We described a novel immunocytochemical method for the identification of Acanthamoeba spp. based on selective binding of Clostridium cellulovorans cellulase to protozoan cyst wall cellulose. Free-living amoebae isolated from different water sources by filtration and subsequent cultivation on non-nutrient agar were assigned to genera Acanthamoeba, Naegleria or Hartmannella using morphological taxonomic criteria. Tissues samples from experimentally infected mice were fixed in formalin and for sectioning embedded in paraffin or snap frozen. The Cellulose-Binding Domain of C. cellulovorans cellulase (CBD) obtained as a recombinant protein, were coupled to the fluorescent dye using Alexa Fluor®350, 488, 568 – Protein Labelling Kit or labelled with the biotin using EZ-Link Sulfo-NHS-Biotin. All coupling procedures were performed according to the methods provided by manufacturers. For staining with CBD conjugate, slides containing cysts collected from the agar plates or tissue sections were immersed with PBS and incubated with CBD for 30 min at room temperature, washed 3 times with PBS. For staining with CBD-biotin slides containing cysts were incubated with biotinylated CBD for 30 min at room temperature. Subsequent washings in changes of PBS were followed by the incubation with Strept ABComplex/HRP, for 30 min at room temperature, than 3,3 diaminobenzidine tetrahydrochloride was added for 15 min. Slides were rinsed with water, dried and examined in the light microscope. We showed that cellulose could be easily detected by immunofluorescence using conjugated CBD in the inner cyst wall of Acanthamoeba spp. The reference strains of Acanthamoeba spp. and all Acanthamoeba strains isolated from water and from tissues of infected animals gave positive reaction. CBD prepared as a biotynylated protein can be also used for the demonstration of Acanthamoeba cyst in infected tissues and environmental samples.
    Źródło:
    Wiadomości Parazytologiczne; 2009, 55, 1; 47-51
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wykrywanie obecności Acanthamoeba sp. w próbach środowiskowych i materiale pochodzącym od pacjentów przy pomocy techniki PCR
    Autorzy:
    Derda, M.
    Hadaś, E.
    Sułek, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147593.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    proby srodowiskowe
    material kliniczny
    parazytologia lekarska
    Acanthamoeba
    lancuchowa reakcja polimerazy
    metody wykrywania
    ameby
    pasozyty czlowieka
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 4; 747
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pełzaki wolno żyjące jako nosiciele patogenicznych bakterii
    Free-living amoebae as vehicles of pathogenic bacteria
    Autorzy:
    Derda, M.
    Sułek-Stankiewicz, A.
    Hadaś, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144083.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zapalenie rogowki
    Balamuthia mandrillaris
    Sappinia diploidea
    zapalenie opon mozgowych i mozgu
    choroby czlowieka
    Acanthamoeba
    bakterie patogenne
    ameby
    Naegleria
    nosiciele
    ziarniniakowe zapalenie mozgu
    Opis:
    The free-living amoebae are ubiquitous organisms. They are found in humid soil and all water reservoirs, i.e. fresh, sea, freezing and hot water. They mainly feed on bacteria. Pathogenic properties of amoebae and the mechanisms underlying pathological changes induced during human infection have not yet been fully elucidated. They are the causative agents of primary amoebic meningo-encephalitis (PAM), granulomatous amebic encephalitis (GAE), a chronic progressive disease of the central nervous system, amebic keratitis (AK), a chronic eye infection; amebic pneumitis (AP), a chronic lung infection, and skin infection. Only a few isolates are strongly and permanently pathogenic to humans. Some isolates lose their pathogenic properties after one passage. It has been assumed that such "temporary", unstable pathogenic properties of the amoebae may be caused by internal factors carried by them. It is generally known that the free-living amoebae may be naturally infected with pathogenic bacteria, which have the ability to survive for a long time and to proliferate in the amoebae cells. The role of the amoeba in the process of maintaining, propagating and transmitting human pathogens has not been well recognized. It has been suggested that some infections can be acquired by inhaling aerosols containing amoebae cells filled with bacteria. The presence of bacteria inside the free-living amoebae possess a great challenge to organisations responsible for testing and inspecting the quality and cleanliness of surface waters, swimming pools and drinking water intakes.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 1; 1-7
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Extracerebral infections of Acanthamoeba spp. in mice. Pathomorphological changes in tissues of infected animals
    Autorzy:
    Mazur, T.
    Gustowska, L.
    Hadaś, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148734.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    infection
    mice
    animal
    Poznan city
    central nervous system
    parasite
    Acanthamoeba
    extracerebral infection
    mouse
    pathomorphological change
    amoeba
    kidney
    heart
    liver
    lung
    infected animal
    tissue
    Opis:
    A variety of Acanthamoeba spp. pathogenic strains were isolated from water pools in the area of Poznań. In many cases amoebae invaded lugs, kidney heart, liver and diaphragm causing significant changes in these Organs. The presence of amoeba outside of CNS was found mainly in the early periods of infection. Amoeba isolated from different organs of dead animals did not exhibit any specificity in relation to the organs in following infections. Infection with both low-and high-virulent strains resulted in pathomorphological changes of similar character. However, changes induced by low-virulent parasites developed later and were less extensive.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 1; 83-87
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pełzakobójcze ekstrakty roślinne
    Amebicidal plants extracts.
    Autorzy:
    Derda, M.
    Hadaś, E.
    Thiem, B.
    Sułek, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147585.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    olownik latkowaty
    Solidago virgaurea
    Acanthamoeba
    malina moroszka
    nawloc pospolita
    Rubus chamaemorus
    Pueraria lobata
    ekstrakty roslinne
    pasozyty czlowieka
    zwalczanie pasozytow
    nawloc waskolistna
    czynniki chorobotworcze
    parazytologia lekarska
    aktywnosc pasozytobojcza
    ameby
    Solidago graminifolia
    ziarniniakowe zapalenie mozgu
    Opis:
    The free-living amoebae from genus Acanthamoeba are the causative agents of granulomatous amebic encephalitis (GAE), a chronic progressive disease of the central nervous system; amebic keratitis (AK), a chronic eye infection; amebic pneumitis (AP), a chronic lung infection, and skin infection. Chemotherapy of Acanthamoeba infection is problematic. The majority of infections have been fatal. Only a few cases are reported to have been treated successfully with very highly toxic drugs. The therapy might be succeed, if the diagnosis and therapy is made at very early stage of infection. In our experiments we used the following plant extracts: Solidago virgaurea, Solidago graminifolia, Rubus chamaemorus, Pueraria lobata, and natural plants products as ellagic acid and puerarin. Those therapeutic agents and plants extracts have been tested in vitro for amebicidal or amebostatic activity against pathogenic Acanthamoeba spp. Our results showed that methanol extracts obtained from plants are active against axenic pathogenic Acanthamoeba sp. trophozoites in vitro at concentration below 0.1 mg/ml. Further studies are needed to investigate whether these extracts are also effective in vivo in animal model of infection with Acanthamoeba sp.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 4; 715-721
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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