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Wyświetlanie 1-15 z 19
Tytuł:
Klinika i epidemiologia toksokarozy
Autorzy:
Pawłowski, Z.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/2151574.pdf
Data publikacji:
1993
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toksokaroza
choroby inwazyjne
parazytologia
konferencje
leczenie
epidemiologia
Warszawa konferencja
Źródło:
Wiadomości Parazytologiczne; 1993, 39, 1; 118-122
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Konferencja naukowo-szkoleniowa pt.: Bionomia i kontrola pasożytow zewnętrznych
Autorzy:
Złotorzycka, J.
Powiązania:
https://bibliotekanauki.pl/articles/2151575.pdf
Data publikacji:
1993
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
Wroclaw konferencja
pasozyty zewnetrzne
parazytologia
konferencje
bionomia
Źródło:
Wiadomości Parazytologiczne; 1993, 39, 1; 122-124
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane aspekty patologii klinicznej włośnicy
A selected aspects of clinical pathology of human trichinellosis
Autorzy:
Kocięcka, W.
Powiązania:
https://bibliotekanauki.pl/articles/2144341.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
czynniki chorobotworcze
choroby pasozytnicze
patologia kliniczna
choroby czlowieka
parazytologia
Trichinella
konferencje
pasozyty
Warszawa konferencja
Opis:
In this review the pathomechanisms of human trichinellosis with particular relation to intestinal and muscular invasion are presented. The numerous factors initiated by antigen released by Trichinella, which play a role in the development of pathological process (including immunological, pathomorphological, metabolic and bioelectric disturbances) and short clinical characteristics of the disease are also presented. Pathology of late period of trichninellosis sequelae are discussed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 189-198
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Długosz, E.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144424.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Różnicowanie izolatów Malassezia pachydermatis metodą RAPD
RAPD differentiation of yeast like fungi Malassezia pachydermatis
Autorzy:
Leśniak, M.
Dworecka-Kaszak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2144426.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Malassezia
diagnostyka parazytologiczna
grzyby
metoda RAPD
Malassezia pachydermatis
parazytologia
konferencje
roznicowanie
izolaty grzybowe
Warszawa konferencja
Opis:
The aim of the work was analyzing of genomic DNA of Malassezia pachydermatis isolates from clinical cases otitis externa from dogs using RAPD method with arbitrary primers Eric1R, Eric2, BG2 and FM1. Materials and methods. 47 strains of M. pachydermatis isolates from clinical cases otitis externa from dogs were tested. Isolation of genomic DNA was provided according with MasterPureTM Yeast DNA Purification Kit EPICENTRE procedure. The quality of isolated genomic DNA was determined electrophoreticaly. For differentiation the following primers were used: Eric1R, Eric2, BG2 and FM1. Primers Eric 1R and Eric 2 were used together in one reaction or amplificated separately. Obtained products were analyzed electrophoreticaly in 1.5% agarose gel. For determination of phylogenic tree Quantity one VersaDoc (BioRad) and Statgraphics plus 4.1 programs were used. Results. High degree of heterogeneity of DNA among investigated isolates of M. pachydermatis was shown using FM1 primer. Dendrograms were prepared by calculation euclid's distance of different parameters (size and count of RAPD products) by nearest neighbor method. Basing on phylogenic tree four main types (phylogenic groups) of M. pachydermatis isolates were shown. The other five groups non-count was shown also.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 299-304
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologia włośnicy w Polsce dawniej i dziś
Epidemiology of human trichinellosis in Poland - currently and in the past
Autorzy:
Gołąb, E.
Sadkowska-Todys, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144340.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
historia
Polska
pasozyty
nicienie
epidemiologia
Warszawa konferencja
wystepowanie
choroby czlowieka
parazytologia
konferencje
Trichinella
Opis:
Since the XIX century human trichinellosis has remained an unsolved problem of public healthcare in Poland. This paper describes the past situation and analyses current changes in the epidemiological pattern of trichinellosis in Poland. Epidemiological data from the last 60 years, point out that the number of human cases as well as the number of deaths caused by trichinellosis has decreased significantly. Up to 90s the main source of Trichinella infection for people was pork. Among other implemented control measures, the introduction of the artificial digestion method in the early 80s to detect trichinellosis in pigs resulted in a shift in the sources of Trichinella infection in humans - pork was replaced with wild boar meat. In the years 1990-1995 the number of outbreaks due to pork consumption was 3.5-times higher than in the years 2000-2005. In the early nineties pork was the source of infection causing about 71% of all outbreaks; in 2000-2005 that number has fallen to only 12%. On the other hand wild boar meat was responsible for 23% of the outbreaks in 1990-1995 and as many as 88% of all outbreaks in the years 2000-2005. Moreover the number of persons infected in the outbreaks significantly decreased. The study of wild animals demonstrated that wild boars in Poland are infected not only with T. spiralis but also with Trichinella britovi. These results and EU recommendations indicate a requirement of determining the Trichinella species which cause infections in outbreaks. In the 3 trichinellosis outbreaks in 2005 the infected meat products were examined with molecular tools. T. spiralis species larvae were the etiological agents of infection in all these outbreaks. The current epidemiological situation of trichinellosis in Poland indicates a need of increasing the awareness of risks related to wild boar meat consumption among the general public. Introducing the artificial digestion method as an obligatory method for wild boar meat examination is also necessary.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 181-187
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie Giardia intestinalis u psów domowych w Warszawie
Prevalence of Giardia intestinalis in domestic dogs in Warsaw
Autorzy:
Zygner, W.
Jaros, D.
Skowrońska, M.
Bogdanowicz-Kamirska, M.
Wędrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/2144594.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
pasozyty zwierzat
psy
diagnostyka parazytologiczna
choroby pasozytnicze
Giardia intestinalis
parazytologia
konferencje
giardioza
Warszawa
choroby zwierzat
Warszawa konferencja
Opis:
Introduction. Giardia intestinalis is the most common intestinal protozoan parasite, which infects humans, dogs and other mammals throughout the world. So far eight genotypes of the parasite have been described of which four were found in dogs. Assemblages A-I and B infect either dogs or humans. Assemblages C and D occur only in dogs. The aim of the study was to determine the prevalence and genotypes of G. intestinalis in domestic dogs of Warsaw area. Material and methods. From October 2005 to March 2006 fecal samples were collected from 350 dogs and examined using light microscopy and PCR techniques. Results. 5.14% of dogs was found to be positive for G. intestinalis by microscopy and prevalence of 9.14% was found by PCR. The PCR amplicons were sequenced and the DNA sequences were compared with Giardia sequences in GeneBank database. The analysis revealed assemblage A-I in 1.71% of dogs, assemblage C in 1.14% and assemblage D in 6.28% of dogs in Warsaw. According to literature, the genotype A can infect humans however a role of dogs as a reservoir of human giardiosis in Poland is not known.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 311-315
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pasożyty przewodu pokarmowego krolików w badaniach koproskopowych
Gastrointestinal parasites of rabbits in coproscopic investigations
Autorzy:
Nosal, P.
Petryszak, A.
Nowosad, B.
Sobolewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2146335.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
diagnostyka parazytologiczna
przewod pokarmowy
choroby pasozytnicze
ziarniaki
parazytologia
kroliki
konferencje
nicienie
badania koproskopowe
Warszawa konferencja
Opis:
Background. Parasitological screening was carried out in a breeding herd of the New Zealand White rabbit breed in 2004. Material and methods. Mothers, together with their offsprings, were kept in boxes on deep litter, whereas young rabbits were transferred to cages with slatted floor. All the animals were fed a complete balanced pelleted feed with two coccidiostats added alternately (Lerbec, Robenidine), and droplet watering system was applied. No anthelmintic was used in the rabbitry. A total of 170 individual samples of fresh feces from females of breeding stock, and 55 pooled samples in the case of young animals 2-4 months of age, collected on a month intervals, were analyzed according to a modified concentration McMaster's method, using saturated salty water with sugar as a flotation fluid. Results. The presence of several species of coccidia was evaluated, with Eimeria stiedae, E. perforans, E. media and E. magna being found in great numbers. The dynamics of infection showed that both adults and young animals were affected by coccidia mostly in May (mean intensity reached at that time 24000 opg in young rabbits), and then until August the infection lasted on a high level. As regards coccidiosis, parasitism could be considerably cut back in the herd by frequent changing of the straw litter, which should also always be dry. Only Passalurus ambiguus was found from nematodes, and only in the feces of adults, probably due to the modern forage and watering system applied, which broke the other worms' life cycles.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 327-330
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ szczepienia cieląt rekombinowaną proteazą cysteinową Fasciola hepatica na rozwój i inwazyjność miracydiów
Influence of vaccination of calves with recombinant cysteine proteinase of Fasciola hepatica on development and infectivity of miracidia
Autorzy:
Dąbrowska, M.
Kaliniak, M.
Wędrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/2144561.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka parazytologiczna
bydlo
inwazyjnosc
cieleta
Warszawa konferencja
proteazy cysteinowe
choroby inwazyjne
Fasciola hepatica
parazytologia
konferencje
szczepionki przeciwpasozytnicze
choroby zwierzat
fascioloza
Opis:
The aim of the study was to evaluate an influence of vaccination of the final host on F. hepatica development in intermediate hosts. Fluke eggs were isolated from the biliary tracts of calves vaccinated orally with recombinant cysteine proteinase of F. hepatica after the challenge infection and from control calves which received the infection only. To asses the ef fect of the vaccine on egg "hatch rate" the eggs were transferred to the Petri dishes with distilled water and incubated at 25°C for 16-19 days. They were subsequently exposed to light for about 2 h, at a temperature of 27 ± 1°C, to stimulate sprouting of the miracidia and asses the egg hatchability. In order to evaluate infectivity and pathogenicity of the miracidia, single miracidium infections of Lymnea truncatula by F. hepatica were carried out under laboratory conditions using 4-mm-high snails. The prevalence of snail infections with F. hepatica was calculated using the ratio between the number of cercariae-shedding snails in each group and that of surviving snails. It appeared that the eggs isolated from immunized calves demonstrated significantly lower hatchability than the eggs isolated from non-vaccinated control hosts. Also, the proportion of infected snails as well as their mortality were lower after exposition to miracidia originating from vaccinated calves. It is suggested that effectors of the immune response in vaccinated calves inhibited in part biological activity of cysteine proteinases of the fluke which are known to be involved in egg shell formation, penetration of host's tissues and worm feeding.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 305-309
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biologia, zróżnicowanie gatunkowe i rozprzestrzenianie nicieni z rodzaju Trichinella
Biology, species biodiversity and distribution of Trichinella nematodes
Autorzy:
Moskwa, B.
Powiązania:
https://bibliotekanauki.pl/articles/2144337.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty wewnetrzne
choroby czlowieka
biologia populacji
rozprzestrzenianie sie pasozytow
parazytologia
zroznicowanie gatunkowe
Trichinella
konferencje
nicienie
badania filogenetyczne
zakazenia pokarmowe
Warszawa konferencja
Opis:
From the time of the discovery of Trichinella larvae in 1835 until the middle of the next century it was commonly assumed that all trichinellosis was caused by a single species Trichinella spiralis. This species is an intracellular parasite in both a larva and an adult stage. The L1 larvae live in a modified skeletal muscles. The adult worms occupy a membrane-bound portion of columnar epitelium, living as intramulticellular parasite. More than century later T. spiralis have been reported from more than 150 different naturally or experimentally infected hosts and demonstrated worldwide distribution in domestic and/or sylvatic animals. Up to date, Trichinella genus comprised eight species (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T. pseudospiralis, T. papuae and T. zimbabwensisi) and three additional genotypic variants that have not yet to be taxonomically defined (T6, T8, T9). Molecular markers revealed that Trichinella T6 is related to T. nativa, Trichinella T8 related to T. britovi. Two main clades are recognized in the genus Trichinella: the first encapsulated in host muscle tissue and the second-non-encapsulated. In this paper the history of Trichinella spp. discovery, their life cycle, taxonomy and phylogeny have been reviewed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 157-164
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatność ELISA do diagnozowania włośnicy u świń i dzików
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144342.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poszukiwanie antygenów wzbudzajacych odporność przeciwko inwazjom tegoryjcow
Search for protective antigens of hookworms
Autorzy:
Wiśniewski, M.
Długosz, E.
Bąska, P.
Wędrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/2144425.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka parazytologiczna
choroby pasozytnicze
tegoryjce
odpornosc zwierzat
perspektywy
Warszawa konferencja
choroby inwazyjne
objawy chorobowe
parazytologia
konferencje
choroby zwierzat
antygeny
szczepionki
Opis:
Hookworms are very important blood sucking nematode parasites of humans and domestic animals. The host with a heavy infection can lose almost a cup of blood per day. This may contribute to anemia which is associated with many physical and mental developmental insults. The works on obtaining an effective hookworm vaccine have been lasting for about eighty years. Recent identifications of a number of bioactive molecules produced by larval and adult stages of Ancylostomatidae are very helpful for selecting of nematode proteins crucial for host-parasite interactions and promising vaccine antigens. Many of these molecules are involved in host skin penetration by infective larvae, intestinal tissue invasion and digestion of haemoglobin and/or other macromolecular substrates. However, the results of many vaccination trials using recombinant forms of these proteins showed no sufficient protection against experimental hookworm infections.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 271-276
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzęta wolno żyjace i domowe jako stale utrzymujacy się rezerwuar włośnicy w Polsce
Wild and domestic animals as permanent Trichinella reservoir in Poland
Autorzy:
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/2144339.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
Polska
pasozyty
nicienie
Warszawa konferencja
zwierzeta dziko zyjace
zywiciele
Trichinella britovi
wystepowanie
wlosien krety
choroby czlowieka
zwierzeta domowe
parazytologia
Trichinella spiralis
konferencje
Opis:
Backround. Since Owen first described Trichinella as a human patogen in 1835, the number of organisms comprising this genus has grown dramatically. This etiological agent of human trichinellosis shows worldwide distribution in domestic and/or sylvatic animals. Material and method. The aim of the presented paper was to determine the distribution of Trichinella species in wild animals such as red foxes, wolves, wild boars, and domestic pigs in Poland. Muscle samples from diaphragm and forelegs were collected from animals killed by hunters. Muscle larvae were recovered from the muscle after artificial digestion and identified at the species level by RAPD, PCR-RPLF and multiplex PCR. Results. Of 75 nematode isolates from red foxes: (Vulpes vulpes), 50 resulted as T. britovi, 6 T. spiralis, 6 were mixed infections of these two species. Fifteen Trichinella isolates remained unidentified. Of 97 nematode isolates from wild boars (Sus scrofa), 21 resulted as T. britovi, 69 T. spiralis, 2 were mixed infections of these two species. Five Trichinella isolates remained unidentified. Of 6 examined wolves (Canis lupus) killed in the Bieszczady region, 3 animals were positive against T. britovi. Of 6 examined raccon dogs (Nyctereutes procyonoides) from Pomorskie region, 2 animals were positive against T. spiralis. Of 21 nematode isolates from domestic pigs, 1 resulted as T. britovi and 21 as T. spiralis. Up to date, two Trichinella species are detected as the etiological agents of epidemiology among domestic and wildlife animal in Poland: T. britovi is the dominant species in red foxes and T. spiralis is the dominant species in wild boars and domestic pigs.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 175-179
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144343.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 19

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